Breeding method of maize parthenogenesis inducer and its application in maize inbred line breeding

A parthenogenesis and induction line technology, applied in the field of crop breeding, can solve the problems of the low induction rate of Stock6, restricting the large-scale and high-efficiency application of the technology, and the defects of reproductive performance, so as to improve the breeding efficiency, reduce the breeding period, shorten the Effects of Breeding Cycles

Inactive Publication Date: 2012-05-09
GUANGXI ZHUANG AUTONOMOUS REGION CORN RES INST
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Problems solved by technology

[0014] The large-scale and high-efficiency application of this technology is restricted

Method used

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  • Breeding method of maize parthenogenesis inducer and its application in maize inbred line breeding
  • Breeding method of maize parthenogenesis inducer and its application in maize inbred line breeding
  • Breeding method of maize parthenogenesis inducer and its application in maize inbred line breeding

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Embodiment 1

[0033] Embodiment 1, a kind of breeding method of maize parthenogenetic induction line

[0034] Step 1: In the spring of 2008, use the maize material DMFC04.662 with excellent disease-resistant traits such as resistance to large and small leaf spot and head smut to construct the basic population of maize parthenogenic induction lines (DMFC04.662 × Nongda Gaoyu No. 1 ). The parents of the basic group DMFC04.662 and Nongda Gaoyu No. 1 were donated by China Agricultural University (DMFC04.662 and Nongda Gaoyu No. 1 can be purchased by contacting China Agricultural University).

[0035] Step 2: The main agronomic traits were selected by the pedigree method on the backcross or self-crossed segregation progenies (backcross first generation, self-cross first generation to sixth generation) of the basic population F1 (DMFC04.662×Nongda Gaoyu No. 1). The main agronomic traits include: plant ABP1 purple marker, plant height, number of male flower branches, pollen characteristics, polle...

Embodiment 2

[0044] Embodiment 2, the method for fast, efficient and large-scale selection and breeding of high-purity corn inbred lines using corn parthenogenetic induction lines, comprising the following steps:

[0045] 1. Induce haploid. Using the parthenogenetically induced line Guiyu No. 1 as the male parent to induce cross-pollination of a large number of corn breeding germplasm resources that meet the breeding objectives, harvest the ears on the female plants of the current season, and use the color markings on the ears for identification and classification , showing that the aleurone layer at the top of the endosperm has purple markings, but the grains without purple markings at the top of the germ are pseudo-haploid grains.

[0046] 2. Chromosome doubling of haploid seedlings. The pseudo-haploid grains obtained in step 1 are finely sown, and the haploid seedlings are confirmed by using the agronomic traits such as leaf sheath color, seedling or plant growth and growth from the se...

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Abstract

The invention discloses a breeding method of a maize parthenogenesis inducer and its application in maize inbred line breeding. Employment of a maize parthenogenesis inducer can induce a female parent material to perform parthenogenesis and generate a female parent haploid, thus realizing a method able to rapidly breed a highly homozygous maize inbred line in a large-scale through simple process and create a lot of new maize breeding resources. And the application of a maize parthenogenesis inducer consists of: using a high frequency parthenogenesis inducer Guiyou No.1 as a male parent to hybridize with an excellent maize breeding germplasm resource, making use of an obvious seed purple marker to pick out a haploid grain, and utilizing the purple marker of a plant to distinguish a non-haploid plant, subjecting a haploid plant to chromosome doubling so as to become a normal diploid, then conducting inbreeding so as to generate a new highly homozygous maize inbred line, which can be applied in maize hybrid combination preparation for breeding new maize varieties. Compared with a traditional pedigree breeding method, the method provided in the invention can shorten the breeding time of an inbred line by 3-4 years, thus shortening the breeding period of new maize varieties.

Description

technical field [0001] The invention relates to the breeding of corn parthenogenetic induction lines and the application in breeding corn inbred lines, belonging to the technical field of crop breeding. Background technique [0002] Corn is the largest food crop in my country. It has the characteristics of high biological yield per unit area and grain yield, huge potential for yield increase and genetic improvement, wide adaptability, rich nutrition, and wide application in animal husbandry, industry and other industries. important role in food security. [0003] In the historical process of world grain production development, as the land resources available for crop production are dwindling, increasing per unit yield has become the primary way to increase total grain production. The experience of genetic improvement of maize shows that genetic improvement is the most influential factor on the increase of maize yield per unit area, and its effect is about 35% to 40%. After ...

Claims

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Application Information

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IPC IPC(8): A01H1/02
Inventor 江禹奉程伟东周锦国覃兰秋谭贤杰谢和霞
Owner GUANGXI ZHUANG AUTONOMOUS REGION CORN RES INST
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