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Parthenogenic activation of human oocytes for the production of human embryonic stem cells

A technology of oocytes and parthenogenesis, applied in the field of embryonic stem cells, can solve human concerns and other problems

Inactive Publication Date: 2009-09-16
INT STEM CELL CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, ethical concerns regarding the origin of hES and hEG cells, and the concern that using NT for research could lead to human production using NT, have generated considerable public discussion and debate.

Method used

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  • Parthenogenic activation of human oocytes for the production of human embryonic stem cells
  • Parthenogenic activation of human oocytes for the production of human embryonic stem cells
  • Parthenogenic activation of human oocytes for the production of human embryonic stem cells

Examples

Experimental program
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Embodiment 1

[0158] Generation of human parthenogenetic embryogenic stem cells

[0159] [0151] Materials and Methods

[0160] [0152] The donor voluntarily donated oocytes, cumulous cells, and blood (for DNA analysis) without paying money. Donors signed full informed consent documents and were informed that all donated material was used for research and not for reproductive purposes. Prior to ovarian stimulation, according to FDA Qualified Determination Guidelines for Human Cells, Tissues, and Cell- and Tissue-Based Products (Food and Drug Administration. (Draft) Guidance for Industry: Eligibility Determination for Donors of Human Cells, Tissues, and Cellular and Tissue Based Products (HCT / Ps), May 2004) and the Russian Ministry of Public Health No. N67 (02.26.03), oocyte donors are examined for medical fitness. It includes X-rays, blood and urinalysis, and liver function tests. Donors are also screened for syphilis, HIV, HBV, and HCV.

[0161] [0153] Oocytes are obtained using standa...

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Abstract

Methods of producing human stem cells by parthenogenetically activating human oocytes by manipulation of oxygen tension, including manipulation of Ca2+ under high 02 tension using anionophore and contacting oocytes with serine threonine kinase inhibitors (DMAP) under low 02 tension, isolating inner cell masses (ICMs) from the activated oocytes, and culturing the cells of the isolated ICMs under high 02 tension. Moreover, methods are described for the production of stems cells from activated oocytes in the absence of non-human animal products, including the use of human feeder cells / products for culturing ICM / stem cells. Stem cells produced by the disclosed methods are also described.

Description

technical field [0001] [0001] The present invention relates generally to embryonic stem cells and, more particularly, to methods of obtaining human embryonic stem cells using parthenogenically activated oocytes. Background technique [0002] [0002] Human embryonic stem cells (ES) are pluripotent cells capable of differentiating into a large number of cell types. When embryonic stem cells are injected into immunodeficient mice, the embryonic stem cells form differentiated tumors (teratomas). However, embryonic stem cells induced to form embryonic bodies (EBs) in vitro provide a source of embryonic stem cell lines that, under certain growth conditions, can differentiate into a variety of cell types of several tissues feature. For example, ES cells differentiate into neurons in the presence of nerve growth factor and retinoic acid. [0003] [0003] Human ES cells and their differentiated progeny are an important source of normal human cells for therapeutic transplantation and...

Claims

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Application Information

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IPC IPC(8): C12N5/00
Inventor E·S·雷瓦佐娃M·V·普吕兹科娃L·N·库兹米乔夫J·D·贾纳斯
Owner INT STEM CELL CORP
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