Special-effect foliar fertilizer for improving cold resistance of durian seedlings as well as preparation method and application of special-effect foliar fertilizer

Abstract

The invention discloses a special-effect foliar fertilizer for improving cold resistance of durian seedlings as well as a preparation method and application of the special-effect foliar fertilizer, and belongs to the technical field of durian planting. The special-effect foliar fertilizer prepared by the invention is prepared from the following raw materials in parts by weight: 2 to 5 parts of fulvic acid, 10 to 20 parts of antifreeze agent, 0.5 to 2 parts of pine needle extract, 3 to 8 parts of alginate oligosaccharide, 0.3 to 0.8 part of 14-hydroxybrassinosterol, 0.2 to 0.5 part of 0.01 to 0.1 percent v / v polysorbate-20, 2 to 4 parts of chitosan, 1 to 3 parts of trehalose, 0.1 to 1 part of brassinolide, 0.1 to 0.3 part of salicylic acid and 6 to 10 parts of glycerol. The special-effect foliar fertilizer is simple to prepare, safe overwintering of durian seedlings at the low temperature of 4-15 DEG C can be effectively guaranteed, and the problems of dead seedlings and runt seedlings caused by low temperature are remarkably solved. The durian seedling culture medium is safe and environment-friendly in components, is beneficial to reduction of environmental pollution and pesticide residues, provides rich nutrition for breeding and growth of durian seedlings, and has remarkable application potential for promoting development of the durian industry.

Description

Technical Field

[0001] The invention relates to the technical field of durian planting, and in particular to a special-effect foliar fertilizer capable of improving the cold resistance of durian seedlings, and a preparation method and application thereof. Background Art

[0002] Durian (Durio zibethinus) is a tropical fruit with high nutritional and economic value. However, durian has high requirements for its growth environment. In particular, low temperatures significantly inhibit its physiological activities, leading to reduced fruit quality, yield, and even plant death. Therefore, improving durian's cold tolerance has become a key issue in the durian cultivation industry.

[0003] At present, traditional cold-proof measures such as covering method and fumigation method cost up to more than 30,000 yuan per mu, and have limited effect. Although prior art CN117204436A discloses tea tree antifreeze agent, its main component abscisic acid can suppress the flower bud differentiation of durian. The corn antifreeze agent reported by CN109717027A and the green pepper antifreeze agent reported by CN117378625A etc. are all for specific plants, but different plants, their cold resistance is mostly different, therefore for different plants, need to find specific antifreeze formula, thereby effectively guarantee that plant safely survives the winter under certain low temperature conditions. Yet, at present specifically for improving the cold resistance of durian seedlings not yet found. Therefore, in the urgent need to develop a kind of special foliage fertilizer for improving the cold resistance of durian seedlings. Summary of the Invention

[0004] The main purpose of the present invention is to provide a special-effect foliar fertilizer for improving the cold resistance of durian seedlings, as well as a preparation method and application thereof. The special-effect foliar fertilizer is simple to prepare, can effectively ensure that durian can safely survive the winter under low temperatures of 4-15°C, thereby effectively solving the problems of low yield and poor quality of durian caused by low temperatures. The ingredients are safe and environmentally friendly, and have a positive effect on reducing environmental pollution and reducing pesticide residues.

[0005] In order to achieve the above technical objectives, the technical solution adopted by the present invention is:

[0006] A special-effect foliar fertilizer for improving the cold resistance of durian seedlings and a preparation method thereof. The preparation method of the special-effect foliar fertilizer comprises the following steps: mixing liquid A and liquid B in a ratio of 1:1, adding deionized water to make up to 100 parts, ultrasonically dispersing for 30-60 minutes, and mixing evenly to obtain the special-effect foliar fertilizer, wherein:

[0007] Liquid A is composed of the following raw materials in parts by weight: 2-5 parts of fulvic acid, 10-20 parts of antifreeze;

[0008] Liquid B is composed of the following raw materials in parts by weight: 0.5-2 parts of pine needle extract, 3-8 parts of brown algae oligosaccharide, 0.3-0.8 parts of 14-hydroxybrassinosteroid, and 0.2-0.5 parts of 0.05% v / v polysorbate.

[0009] Furthermore, the preparation method of the antifreeze agent is:

[0010] 3 parts of chitosan, 2 parts of trehalose, 0.5 parts of brassinolide, 0.2 parts of salicylic acid, and 8 parts of glycerol were weighed in sequence, and deionized water was added to make up to 100 parts. The mixture was stirred evenly to obtain an antifreeze agent.

[0011] Furthermore, the preparation method of the liquid A is as follows:

[0012] Dissolve fulvic acid and antifreeze in deionized water and stir evenly to obtain solution A.

[0013] Furthermore, the preparation method of the B solution is:

[0014] Pine needle extract, brown algae oligosaccharide, 14-hydroxybrassinosteroid and 0.05% v / v polysorbate 20 were dissolved in ethanol aqueous solution and stirred at high speed to obtain solution B.

[0015] Another object of the present invention is to provide an application of a special foliage fertilizer for improving the cold resistance of durian seedlings:

[0016] (1) Dilute 200 times with deionized water before use;

[0017] (2) Spray on the durian tree and leaf surface during the winter cooling period and spring durian budding period, with a spraying rate of 50±5mL / plant;

[0018] (3) Spraying interval is 7±1 days, for 6 consecutive times.

[0019] Furthermore, the ambient humidity during spraying needs to be ≥60%, and the front and back sides of the leaves must be evenly moistened to the critical flow state of the droplets.

[0020] The fulvic acid and 14-hydroxybrassinosteroid in the special-effect foliar fertilizer of the present invention improve the overall growth potential and stress resistance of durian by promoting root development and nutrient absorption; 0.05% v / v polysorbate-20, as a surfactant, not only improves the stability and bioavailability of the special-effect foliar fertilizer, but also ensures the uniform distribution and efficient absorption of each component in the plant body; pine needle extract regulates osmotic pressure, reduces oxidative damage, and improves plant cold resistance; brown algae oligosaccharides can activate antifreeze genes, stabilize cell membranes, and improve plants' low-temperature tolerance; glycerol, as a natural osmotic regulator, further enhances the cold-resistant effect of the special-effect foliar fertilizer.

[0021] Beneficial effects:

[0022] The special-effect foliar fertilizer of the present invention is easy to prepare and can effectively ensure the safe wintering of durian seedlings at low temperatures of 4-15°C. It can significantly improve the cold resistance of durian and reduce the damage to durian plants caused by low temperatures, thereby improving the yield and quality of durian seedlings. Its ingredients are safe and environmentally friendly, helping to reduce environmental pollution and pesticide residues. It also provides rich nutrition for the breeding and growth of durian seedlings, and has significant application potential in promoting the development of the durian industry. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 These are photos comparing the durian phenotypes of the control group and those after treatment with special cold-resistant foliar fertilizer. DETAILED DESCRIPTION

[0024] In order to enable those skilled in the art to better understand the technical solutions of the present invention, the present application will be further described in detail below with reference to the embodiments.

[0025] Example 1

[0026] 1. A special foliar fertilizer for improving the cold resistance of durian seedlings and its preparation method:

[0027] Preparation of Solution A: Dissolve 2 parts of fulvic acid and 10 parts of antifreeze in 40 parts of deionized water and stir well to obtain Solution A.

[0028] Preparation of Solution B: Dissolve 0.5 parts of pine needle extract, 8 parts of brown algae oligosaccharide, 0.8 parts of 14-hydroxybrassinosteroid and 0.5 parts of 0.05% v / v polysorbate 20 in 35 parts of ethanol aqueous solution and stir at high speed for 25 minutes to obtain Solution B.

[0029] Preparation of special-effect foliar fertilizer: Mix liquid A and liquid B in a ratio of 1:1, add deionized water to make up to 100 parts, ultrasonically disperse for 50 minutes, mix evenly, and obtain special-effect foliar fertilizer.

[0030] 2. Application of a special foliar fertilizer in improving the cold resistance of durian seedlings:

[0031] During the winter temperature drop period and spring durian budding, the special effect foliage fertilizer of preparation is diluted with 200 times of deionized water to the tree body and the blade surface of two durian variety nursery stocks of the golden pillow and Mao Shan Wang that normal growth condition is not consistent, seedling age, growth state are close, spraying process, when spraying, form drop with blade surface but do not flow for degree, about 50ml / strain / time, within every 7 days, spray once, spray 6 times continuously, each process 10 strains, 3 repetitions.Control group adopts equal amount of deionized water (50ml / strain / time) to spray, and treatment frequency, environmental condition and observation index are consistent with the special effect foliage fertilizer treatment group.Found that, the durian seedling freezing injury rate, the leaf bud abscission rate of spraying deionized water are all higher, and the durian seedling freezing injury rate, the leaf bud abscission rate after spraying the special effect foliage fertilizer are all than the significant reduction of the durian seedling of spraying deionized water.Result is shown in table 1 below.

[0032] Example 2

[0033] 1. A special foliar fertilizer for improving the cold resistance of durian seedlings and its preparation method:

[0034] Preparation of Solution A: Dissolve 3 parts of fulvic acid and 15 parts of antifreeze in 50 parts of deionized water and stir well to obtain Solution A.

[0035] Preparation of Solution B: Dissolve 1 part of pine needle extract, 5 parts of brown algae oligosaccharide, 0.5 parts of 14-hydroxybrassinosteroid and 0.3 parts of 0.05% v / v polysorbate 20 in 30 parts of ethanol aqueous solution and stir at high speed for 25 minutes to obtain Solution B.

[0036] Preparation of special-effect foliar fertilizer: Mix liquid A and liquid B in a ratio of 1:1, add deionized water to make up to 100 parts, ultrasonically disperse for 50 minutes, mix evenly, and obtain special-effect foliar fertilizer.

[0037] 2. Application of a special foliar fertilizer in improving the cold resistance of durian seedlings:

[0038] During the winter temperature drop period and spring durian budding, the special effect foliage fertilizer of preparation is diluted with 200 times of deionized water to the tree body and the blade surface of two durian variety nursery stocks of the golden pillow and Mao Shan Wang that normal growth condition is not consistent, seedling age, growth state are close, spraying process, when spraying, form drop with blade surface but do not flow for degree, about 50ml / strain / time, within every 7 days, spray once, spray 6 times continuously, each process 10 strains, 3 repetitions.Control group adopts equal amount of deionized water (50ml / strain / time) to spray, and treatment frequency, environmental condition and observation index are consistent with the special effect foliage fertilizer treatment group.Found that, the durian seedling freezing injury rate, the leaf bud abscission rate of spraying deionized water are all higher, and the durian seedling freezing injury rate, the leaf bud abscission rate after spraying the special effect foliage fertilizer are all than the significant reduction of the durian seedling of spraying deionized water.Result is shown in table 1 below.

[0039] Example 3

[0040] 1. A special foliar fertilizer for improving the cold resistance of durian seedlings and its preparation method:

[0041] Preparation of Solution A: Dissolve 5 parts of fulvic acid and 20 parts of antifreeze in 60 parts of deionized water and stir well to obtain Solution A.

[0042] Preparation of Solution B: Dissolve 2 parts of pine needle extract, 3 parts of brown algae oligosaccharide, 0.3 parts of 14-hydroxybrassinosteroid and 0.2 parts of 0.05% v / v polysorbate 20 in 25 parts of ethanol-water solution and stir at high speed for 25 minutes to obtain Solution B.

[0043] Preparation of special-effect foliar fertilizer: Mix liquid A and liquid B in a ratio of 1:1, add deionized water to make up to 100 parts, ultrasonically disperse for 50 minutes, mix evenly, and obtain special-effect foliar fertilizer.

[0044] 2. Application of a special foliar fertilizer in improving the cold resistance of durian seedlings:

[0045] During the winter temperature drop period and spring durian budding, the special effect foliage fertilizer of preparation is diluted with 200 times of deionized water to the tree body and the blade surface of two durian variety nursery stocks of the golden pillow and Mao Shan Wang that normal growth condition is not consistent, seedling age, growth state are close, spraying process, when spraying, form drop with blade surface but do not flow for degree, about 50ml / strain / time, within every 7 days, spray once, spray 6 times continuously, each process 10 strains, 3 repetitions.Control group adopts equal amount of deionized water (50ml / strain / time) to spray, and treatment frequency, environmental condition and observation index are consistent with the special effect foliage fertilizer treatment group.Found that, the durian seedling freezing injury rate, the leaf bud abscission rate of spraying deionized water are all higher, and the durian seedling freezing injury rate, the leaf bud abscission rate after spraying the special effect foliage fertilizer are all than the significant reduction of the durian seedling of spraying deionized water.Result is shown in table 1 below.

[0046] In summary, the cold resistance of durian trees was significantly improved after spraying the special-effect foliar fertilizer on durian seedlings, among which the cold resistance effect of Example 2 was the best, which proved that the present invention was highly feasible and could be widely applied to the production practice of durian seedlings.

[0047] Table 1 Comparative statistics of durian frost damage rate and leaf bud abscission rate

[0048]

Claims

1. A special foliage fertilizer for improving the cold resistance of durian seedlings and a preparation method thereof, characterized in that, The preparation method of the special-effect foliar fertilizer is as follows: liquid A and liquid B are mixed in a ratio of 1:1, deionized water is added to make up to 100 parts, ultrasonically dispersed for 30-60 minutes, and mixed evenly to obtain the special-effect foliar fertilizer, wherein: Liquid A is composed of the following raw materials in parts by weight: 2-5 parts of fulvic acid, 10-20 parts of antifreeze; Liquid B is composed of the following raw materials in parts by weight: 0.5-2 parts of pine needle extract, 3-8 parts of brown algae oligosaccharide, 0.3-0.8 parts of 14-hydroxybrassinosteroid, and 0.2-0.5 parts of 0.05% v / v polysorbate-20.

2. a kind of special effect foliage fertilizer that improves the cold resistance of durian seedlings and preparation method thereof according to claim 1, is characterized in that, The preparation method of the antifreeze agent is: 3 parts of chitosan, 2 parts of trehalose, 0.5 parts of brassinolide, 0.2 parts of salicylic acid, and 8 parts of glycerol were weighed in sequence, and deionized water was added to make up to 100 parts. The mixture was stirred evenly to obtain an antifreeze agent.

3. a kind of special effect foliage fertilizer that improves the cold resistance of durian seedlings and preparation method thereof according to claim 1, is characterized in that, The preparation method of the liquid A is as follows: Dissolve fulvic acid and antifreeze in deionized water and stir evenly to obtain solution A.

4. a kind of special effect foliage fertilizer that improves the cold resistance of durian seedlings and preparation method thereof according to claim 1, is characterized in that, The preparation method of the B solution is: Pine needle extract, brown algae oligosaccharide, 14-hydroxybrassinosteroid and 0.05% v / v polysorbate 20 were dissolved in ethanol aqueous solution and stirred at high speed to obtain solution B.

5. an application of special effect foliage fertilizer in improving the cold resistance of durian seedlings according to any one of claims 1-4, characterized in that: (1) Dilute 200 times with deionized water before use; (2) Spray on the durian tree and leaf surface during the winter cooling period and spring durian budding period, with a spraying rate of 50±5mL / plant; (3) Spraying interval is 7±1 days, for 6 consecutive times.

6. according to the application of a kind of special effect foliage fertilizer in improving durian seedling cold resistance of claim 5, it is characterized in that, When spraying, the ambient humidity needs to be ≥60%, and the front and back of the leaves must be evenly moistened to the critical flow state of droplets.

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