Method for improving germination rate and seedling growth vigor of scutellaria baicalensis seeds in saline-alkali soil environment

Abstract

A method for improving the germination rate and seedling growth vigor of scutellaria baicalensis seeds in a saline-alkali soil environment comprises the following steps: (1) preparing a seed soaking solution, namely preparing a quaternary plant growth regulator combined 6-BA + GA3 + NAA + brassin seed soaking solution; (2) preparing seed dressing mother liquor, namely preparing ternary seed dressing mother liquor of No.6 seed dressing mother liquor, planting elements and pyroligneous liquor; (3) seed soaking: soaking the seeds by using a quaternary seed soaking solution of 6-BA + GA3 + NAA + brassin; (4) airing; (5) seed dressing: carrying out secondary coating by using ternary seed dressing mother liquor of No. 6 seed dressing mother liquor, planting elements and pyroligneous liquor; and (6) sowing: sowing the seeds treated in the step (5) in a matrix containing 0.4% salt stress, and performing conventional culture. Compared with a traditional planting method, the germination rate and seedling growth vigor of the scutellaria baicalensis seeds treated through the method are greatly increased, the germination rate basically reaches 100%, the germination potential is 90%, and the effectiveness and advancement of the method are fully proved.

Description

Technical Field

[0001] The invention belongs to the technical field of agricultural planting, and particularly relates to a method for improving the germination rate of scutellaria baicalensis seeds and the growth potential of seedlings. By performing targeted treatment on scutellaria baicalensis seeds, the seed germination and seedling growth conditions are optimized, and the planting efficiency and quality are improved. Background Art

[0002] As an important Chinese medicinal material with high medicinal value, Scutellaria baicalensis faces many difficulties in the planting process. First, Scutellaria baicalensis seeds themselves have dormancy characteristics, poor seed coat permeability and incomplete embryo development, resulting in a natural germination rate generally lower than 50%; second, even in conventional soil environments, the dense characteristics of its seed coat make the germination rate only 30%-40%, which is difficult to meet the needs of large-scale planting. At present, traditional seed treatment technologies, such as single hormone seed soaking and warm water treatment, can promote seed germination to a certain extent, but the effect is limited. It is impossible to systematically improve the seed germination rate and seedling growth potential, and it is difficult to deal with the germination problems caused by the seeds' own physiological obstacles. Therefore, there is an urgent need for a treatment method that can effectively overcome the germination barriers of Scutellaria baicalensis seeds and significantly improve the germination rate and seedling growth potential. Summary of the Invention

[0003] The present invention aims to address the above-mentioned technical problems and provides a method for improving the germination rate and seedling growth potential of Scutellaria baicalensis seeds in saline-alkali soil environments. Seed treatment is crucial for Scutellaria baicalensis cultivation in saline-alkali soils. The method first prepares a seed soaking solution that activates seed activity and enhances salt-alkali tolerance, along with a seed dressing solution containing germination-promoting and stress-resistant substances. The seeds are then immersed in the soaking solution, controlled for time and temperature to break dormancy. After removal and air-drying, the seeds are thoroughly mixed with the seed dressing solution to form a protective film, thereby improving the germination rate and survival rate of the seeds in saline-alkali environments. The germination rate reaches approximately 100%, and the germination potential reaches 90%.

[0004] To achieve the above object, the technical solution adopted by the present invention is to provide a method for improving the germination rate and seedling growth potential of Scutellaria baicalensis seeds in a saline-alkali soil environment, which comprises the following steps in sequence:

[0005] A method for improving the germination rate and seedling growth potential of Scutellaria baicalensis seeds in a saline-alkali soil environment comprises the following steps in sequence:

[0006] (1) Preparation of seed soaking solution: Prepare a quaternary seed soaking solution of "6-BA+GA3+NAA+brassinolide" by mixing 0.343-0.686 parts of 6-benzylaminopurine mother solution, 0.2-0.5 parts of gibberellin mother solution, 0-0.6 parts of naphthaleneacetic acid mother solution, 0.2-0.4 parts of brassinolide mother solution and 18.293-18.851 parts of deionized water, by volume, to obtain a quaternary seed soaking solution;

[0007] (2) Preparation of seed dressing mother solution: Prepare the three-component seed dressing mother solution of "No. 6 seed dressing mother solution + Yizhisu + wood vinegar" by mixing 0.150-0.183 parts of No. 6 seed dressing mother solution, 0.075-0.2 parts of Yizhisu seed dressing mother solution, and 0.05-0.25 parts of wood vinegar seed dressing mother solution evenly according to volume to obtain the three-component seed dressing mother solution;

[0008] (3) Seed soaking: completely immerse the Scutellaria baicalensis seeds in the soaking solution of step (1) and soak at a constant temperature of 20–25°C for 8–12 h;

[0009] (4) Drying: Allow the soaked seeds to dry naturally in an environment with a temperature of 20–25°C and a relative humidity of 40–50%, turning them over every 1 hour.

[0010] (5) Seed dressing: Use the three-component seed dressing solution of "No. 6 seed dressing solution + Yizhisu + wood vinegar" for secondary coating, fully mix the dried seeds with the seed dressing solution of step (2), and dry them again after the seed surface is evenly coated;

[0011] (6) Sowing: The seeds treated in step (5) were sown in a substrate containing 4‰ salt stress and cultured as usual.

[0012] A method for improving the germination rate and seedling growth potential of Scutellaria baicalensis seeds in a saline-alkali soil environment comprises the following steps in sequence:

[0013] (1) Preparation of seed soaking solution: Prepare a quaternary seed soaking solution of "6-BA+GA3+NAA+brassinolide" by mixing 0.343 parts of 6-benzylaminopurine mother solution, 0.4 parts of gibberellin mother solution, 0.206 parts of naphthaleneacetic acid mother solution, 0.2 parts of brassinolide mother solution and 18.851 parts of deionized water by volume to obtain a quaternary seed soaking solution;

[0014] (2) Preparation of seed dressing solution: Prepare a three-component seed dressing solution of "No. 6 seed dressing solution + Yizhisu + wood vinegar" by mixing 0.183 parts of No. 6 seed dressing solution, 0.075 parts of Yizhisu seed dressing solution, and 0.2 parts of wood vinegar seed dressing solution evenly by volume to obtain the three-component seed dressing solution;

[0015] (3) Seed soaking: completely immerse the Scutellaria baicalensis seeds in the soaking solution of step (1) and soak at a constant temperature of 20–25°C for 8–12 h;

[0016] (4) Drying: Allow the soaked seeds to dry naturally in an environment with a temperature of 20–25°C and a relative humidity of 40–50%, turning them over every 1 hour.

[0017] (5) Seed dressing: Use the three-component seed dressing solution of "No. 6 seed dressing solution + Yizhisu + wood vinegar" for secondary coating, fully mix the dried seeds with the seed dressing solution of step (2), and dry them again after the seed surface is evenly coated;

[0018] (6) Sowing: Sow the seeds treated in step (5) in a medium containing 4‰ salt stress and culture them in a conventional manner.

[0019] After repeated verification of 6 examples and two comparative examples in the laboratory and under simulated saline-alkali soil conditions, the germination rate of Scutellaria baicalensis seeds reached 100% and the germination potential was 90%.

[0020] The No. 6 seed dressing mother solution is a multi-dimensional plant stubble-clearing matrix aqueous solution.

[0021] The constant temperature condition of step (3) is 22°C and the soaking time is 10 h.

[0022] The relative humidity in step (4) is 45%.

[0023] The salt stress in step (6) is caused by the mass volume ratio of 4‰ NaCl and A mixed solution is provided, and NaCl and The mass ratio is 3:1.

[0024] The prepared "6-BA+GA3+NAA+brassinolide" four-component seed soaking solution, wherein 6-BA is 6-benzylaminopurine mother liquor, GA3 is gibberellin mother liquor, NAA is naphthaleneacetic acid mother liquor, and brassinolide mother liquor.

[0025] The beneficial effects of the present invention are:

[0026] 1. Use "6-BA+GA3+NAA+brassinolide" four-element seed soaking solution to soak seeds and "No. 6 seed dressing mother solution+Yizhisu+wood vinegar" three-element seed dressing mother solution for secondary coating. ) design, which significantly improved the germination rate and seedling survival rate of Scutellaria baicalensis seeds in saline-alkali soil environment. The germination rate of Scutellaria baicalensis seeds basically reached 100%, and the germination potential was 90%.

[0027] 2. The dormancy of Scutellaria baicalensis is broken by the synergistic effect of NAA and brassinolide, and the stability of cell membrane under salt stress is improved by secondary coating with wood vinegar, which effectively utilizes saline-alkali land resources and promotes the development of the Scutellaria baicalensis industry in saline-alkali areas.

[0028] 3. After repeated verification of 6 examples and two comparative examples in the laboratory and under simulated saline-alkali soil conditions, the germination rate and seedling indicators were steadily improved.

[0029] 4. The required reagents and equipment are commonly available in the agricultural supply market, can be promoted on a large scale, and are practical. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 Schematic diagram of overall seedling height of Examples 1-6 and Comparative Examples 1-2.

[0031] Figure 2 It is a comparison diagram of Example 3 and Comparative Examples 1-2.

[0032] Figure 3 Schematic diagram for comparison of Examples 2-6.

[0033] Figure 4 Schematic diagram for comparison between Example 1 and Comparative Examples 1-2. DETAILED DESCRIPTION

[0034] The technical solution of the present invention is further described in detail below through specific embodiments.

[0035] The following are Examples 1-6 and 2 comparative examples of the method for improving the germination rate and seedling growth potential of Scutellaria baicalensis seeds in a saline-alkali soil environment according to the present invention, which sequentially comprise the following steps:

[0036] (1) Preparation of seed soaking solution: Using a calibrated pipette, pipette 0.686 ml of 6-BA seed soaking solution, 0.267 ml of GA3 seed soaking solution, 0.514 ml of NAA seed soaking solution, and 0.24 ml of brassinolide seed soaking solution for Example 4 according to Table 1. Pour these solutions into a sterilized plastic container. Then, using an electronic balance with an accuracy of 0.01 g, weigh deionized water based on the amount of water required for 20 ml of seed soaking solution (18.293 g of water required for Example 4), slowly pour the solution into the container, and stir with a sterilized glass rod while adding water. Continue stirring for 3-5 minutes to obtain the seed soaking solution. Label the container with the treatment number.

[0037] (2) Preparation of seed dressing solution: Pipette 0.167ml of No. 6 seed dressing solution, 0.075ml of Yizhisu seed dressing solution, and 0.05ml of wood vinegar seed dressing solution. Transfer the absorbed solution to a glass mixing bottle that has been disinfected with 75% alcohol and dried, mix the solution evenly, and then seal the bottle with plastic wrap and label the treatment number.

[0038] (3) Seed soaking:

[0039] Using an electronic balance with an accuracy of 0.001g, accurately weigh 30g of Scutellaria seeds for each treatment. Place the seeds in a sterilized glass Petri dish and slowly add the soaking solution described in step (1) using a pipette to ensure that the seeds are completely immersed. Place the Petri dish in a constant temperature and humidity incubator set at a constant temperature of 20–25°C and a humidity of 45%. Place a timer in the incubator and gently stir the seeds every 2 hours using alcohol-sterilized tweezers to ensure that the seeds are fully exposed to the soaking solution. Soak for 8–12 hours.

[0040] (4) Drying:

[0041] Remove the soaked seeds from the Petri dish and spread them evenly onto a stainless steel tray that has been sterilized with autoclaved steam and lined with two layers of sterile filter paper. Place the tray in a fume hood with a wind speed set to 0.5 m / s. Allow the tray to air dry at 20–25°C and 40–50% relative humidity. Turn the tray every hour to ensure even drying. Dry for 3–4 hours.

[0042] (5) Seed dressing: Transfer the dried seeds to a plastic seed dressing container that has been sterilized by ultraviolet light for 30 minutes, slowly pour in the seed dressing solution described in step (2), and stir with a sterilized glass rod at a speed of 60-80 rpm for 5-8 minutes to ensure that the seeds fully absorb the seed dressing solution. After the surface of the seeds is evenly coated, spread the seeds on a sterile tray again and put them back into the fume hood to dry. After drying, put the seeds into a sterilized sealed plastic bag, mark the processing number and date, and set aside.

[0043] (6) Sowing: Sow the seeds treated in step (5) in a medium containing 4‰ salt stress and culture them in a conventional manner. Specifically, according to the salt stress concentration requirements, use sodium carbonate ( ) and sodium chloride (NaCl) to prepare a mixed solution. Based on the amount of solution required to fully wet the filter paper in the culture box, accurately calculate the amount of the two salts. Assuming that each culture box needs to be sprayed with 10mL of solution, weigh 0.04g of the salt mixture (10mL x 0.4%). Weigh sodium carbonate and sodium chloride according to a certain ratio (the ratio of sodium carbonate to sodium chloride can be determined according to actual research needs, such as 1:3), dissolve them in deionized water, and stir evenly to prepare a simulated salt stress solution. Transfer the prepared solution to a spray bottle for later use;

[0044] First, prepare the seeds and culture box. Place 100 Scutellaria baicalensis seeds in a sterile culture box. Use a large sheet of germination filter paper and precisely cut it to a uniform length and width, conforming to the box's internal dimensions. Fold the cut filter paper repeatedly lengthwise into a uniform fan shape, ensuring each section is of similar size. Then, lay it evenly on the bottom of the box to provide a good germination substrate.

[0045] Then, evenly spray the simulated salt stress solution into the culture box. Use a handheld sprayer to spray the solution evenly and at a constant angle into each box containing Scutellaria baicalensis seeds until the filter paper is completely moistened and no water accumulates. Carefully control the amount of solution applied to avoid excessive solution, which can cause the seeds to float or rot.

[0046] During the cultivation process, strict daily management is required. Observe the moistness of the filter paper every other day. When the surface of the filter paper appears slightly dry, rehydrate it promptly to maintain the filter paper at a constant moist state, providing a stable moisture environment for seed germination and seedling growth. Furthermore, to ensure uniform light exposure for the seeds within the culture box, rotate the culture box regularly each day so that each seed receives balanced light conditions, thereby promoting uniform seedling growth and reducing growth differences caused by uneven light exposure.

[0047] During the entire operation, all glassware, plastic containers, etc. involved must undergo strict cleaning and disinfection procedures to avoid contamination by impurities and microorganisms.

[0048] Table 1 shows the effects of different plant growth regulator ratios on germination rate and germination potential of Examples 1-6 and Comparative Examples 1-2

[0049] (Experimental environment: using sodium carbonate and sodium chloride to prepare a 0.4% salt solution to simulate salt stress)

[0050] Serial number No. 6 seed dressing solution / μl Yizhisu seed dressing mother solution / μl Wood vinegar seed dressing solution / μl 6-BA seed soaking solution / μl GA3 seed soaking solution / μl NAA seed soaking solution / μl Brassinolide seed soaking solution / μl 20ml of soaking solution needs to add water / ml(g) Germination rate Germination potential Example 1 183 150 200 686 200 0 400 18.714 60% 50% Example 2 150 100 250 343 533 0 280 18.844 85% 80% Example 3 183 75 200 343 400 206 200 18.851 95% 90% Example 4 167 75 50 686 267 514 240 18.293 85% 80% Example 5 158 125 150 686 533 103 360 18.318 70% 60% Example 6 175 200 0 571 533 309 240 18.347 60% 50% Comparative Example 1 0 0 0 0 0 0 0 0 30% 10% Comparative Example 2 0 0 0 0 0 0 0 20 60% 50%

[0051] As can be clearly seen from the data in Table 1, the effects of different plant growth regulator ratios on the germination rate and germination potential of Scutellaria baicalensis seeds are still very significant. Example 3 is the best. According to the experimental conditions of Example 3, the germination rate reached 100% and the germination potential was 90%. All indicators of this treatment were significantly better than those of most other treatments, indicating that this reagent ratio can greatly promote the germination of Scutellaria baicalensis seeds in saline-alkali soil.

[0052] When treated according to the test conditions of Example 2 and Example 4, the germination of Scutellaria baicalensis seeds was also excellent, with a germination rate of 85% and a germination potential of 80%.

[0053] According to the experimental conditions of Example 5, the germination rate of Scutellaria seeds was 70% and the germination potential was 60%. Although the germination rate and germination potential did not reach the optimal level, they still had certain advantages compared with some treatments.

[0054] The germination rate and germination potential of Scutellaria seeds were both low when treated under the experimental conditions of Examples 1 and 6. This indicates that the combination of these two reagents is not conducive to the germination of Scutellaria seeds in saline-alkali soil.

[0055] Generally speaking, when ideal Scutellaria baicalensis seeds are planted in saline-alkali soil, the germination rate is expected to reach more than 80%, and the germination potential is expected to reach more than 60%. This is conducive to the smooth emergence and uniform growth of Scutellaria baicalensis in the saline-alkali soil environment.

[0056] Therefore, according to the data on germination rate and germination potential obtained from Table 1, the reagent ratio scheme of Example 3 performed best in promoting the germination of Scutellaria baicalensis seeds in saline-alkali land, significantly better than other treatments, and better than the performance of the clear water control and the original seed control in a high-salt environment. It is more suitable as a seed treatment scheme for planting Scutellaria baicalensis in saline-alkali land.

[0057] Through experimental comparison, it can be found that different plant growth regulator ratios and treatment methods have a significant effect on seed germination rate and germination potential. Clear water control - the germination rate of the original seed is 60%, and the germination potential is 50%, indicating that under normal conditions without any reagent treatment, Scutellaria seeds have a certain germination ability in saline-alkali land, but compared with the treatments of Example 3, Example 2 and Example 4, the germination potential is weaker. Original seed control - under 4 / 1000 salt treatment, the germination rate and germination potential are both low, indicating that the salt concentration of 4 / 1000 has a great inhibitory effect on the germination of Scutellaria original seed. The experiment proved that the germination rate and seedling growth of Scutellaria seeds treated with the method of the present invention in saline-alkali land were greatly improved compared with the traditional planting method, which fully confirmed the effectiveness and advancement of the method of the present invention.

Claims

1. A method for improving the germination rate and seedling growth potential of Scutellaria baicalensis seeds in a saline-alkali soil environment, characterized in that: The following steps are included in sequence: (1) Preparation of seed soaking solution: Prepare a quaternary seed soaking solution of "6-BA+GA3+NAA+brassinolide" by mixing 0.343-0.686 parts of 6-benzylaminopurine mother solution, 0.2-0.5 parts of gibberellin mother solution, 0-0.6 parts of naphthaleneacetic acid mother solution, 0.2-0.4 parts of brassinolide mother solution and 18.293-18.851 parts of deionized water, by volume, to obtain a quaternary seed soaking solution; (2) Preparation of seed dressing mother solution: Prepare the three-component seed dressing mother solution of "No. 6 seed dressing mother solution + Yizhisu + wood vinegar" by mixing 0.150-0.183 parts of No. 6 seed dressing mother solution, 0.075-0.2 parts of Yizhisu seed dressing mother solution, and 0.05-0.25 parts of wood vinegar seed dressing mother solution evenly by volume to obtain the three-component seed dressing mother solution; (3) Seed soaking: completely immerse the Scutellaria baicalensis seeds in the soaking solution of step (1) and soak at a constant temperature of 20–25°C for 8–12 h; (4) Drying: Allow the soaked seeds to dry naturally in an environment with a temperature of 20–25°C and a relative humidity of 40–50%, turning them over every 1 hour. (5) Seed dressing: Use the three-component seed dressing solution of "No. 6 seed dressing solution + plant-benefiting agent + wood vinegar" for secondary coating, fully mix the dried seeds with the seed dressing solution of step (2), and dry them again after the seed surface is evenly coated; (6) Sowing: The seeds treated in step (5) were sown in a substrate containing 4‰ salt stress and cultured as usual.

2. The method according to claim 1, characterized in that The following steps are included in sequence: (1) Preparation of seed soaking solution: Prepare a quaternary seed soaking solution of "6-BA+GA3+NAA+brassinolide" by mixing 0.343 parts of 6-benzylaminopurine mother solution, 0.4 parts of gibberellin mother solution, 0.206 parts of naphthaleneacetic acid mother solution, 0.2 parts of brassinolide mother solution and 18.851 parts of deionized water by volume to obtain a quaternary seed soaking solution; (2) Preparation of seed dressing solution: Prepare a three-component seed dressing solution of "No. 6 seed dressing solution + Yizhisu + wood vinegar" by mixing 0.183 parts of No. 6 seed dressing solution, 0.075 parts of Yizhisu seed dressing solution, and 0.2 parts of wood vinegar seed dressing solution evenly by volume to obtain the three-component seed dressing solution; (3) Seed soaking: completely immerse the Scutellaria baicalensis seeds in the soaking solution of step (1) and soak at a constant temperature of 20–25°C for 8–12 h; (4) Drying: Allow the soaked seeds to dry naturally in an environment with a temperature of 20–25°C and a relative humidity of 40–50%, turning them over every 1 hour. (5) Seed dressing: Use the three-component seed dressing solution of "No. 6 seed dressing solution + plant-benefiting agent + wood vinegar" for secondary coating, fully mix the dried seeds with the seed dressing solution of step (2), and dry them again after the seed surface is evenly coated; (6) Sowing: The seeds treated in step (5) were sown in a substrate containing 4‰ salt stress and cultured as usual. After repeated verification of 6 examples and two comparative examples in the laboratory and under simulated saline-alkali soil conditions, the germination rate of Scutellaria baicalensis seeds reached 100% and the germination potential was 90%.

3. The method according to claim 1, characterized in that The No. 6 seed dressing mother solution is a multi-dimensional plant stubble-clearing matrix aqueous solution.

4. The method according to claim 1 or 2, characterized in that The constant temperature condition of step (3) is 22°C and the soaking time is 10 h.

5. The method according to any one of claims 1 to 3, characterized in that The relative humidity in step (4) is 45%.

6. The method according to any one of claims 1 to 4, characterized in that The salt stress matrix in step (6) is composed of NaCl and A mixed solution is provided, and NaCl and The mass ratio is 3:1.

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