Crop drought-resistant agent, method for improving crop drought resistance and / or promoting crop growth and development and application of crop drought-resistant agent

Abstract

The invention relates to a crop drought-resistant agent, a method for improving crop drought resistance and / or promoting crop growth and development and application of the crop drought-resistant agent, and belongs to the technical field of agricultural production. The crop drought-resistant agent is prepared from the following components: arginine, chitosan, salicylic acid, diethyl aminoethyl hexanoate, betaine, brassinolide and water. The crop drought-resistant agent can promote normal growth and development of crops under drought conditions, improve plant biomass accumulation and cell antioxidant enzyme activity and reduce toxicity of ROS to the plants, so that adaptability, tolerance and resistance of the crops to drought stress are improved, and the crop drought-resistant agent has the advantages of being good in drought-resistant effect, high in stability and high in safety.

Description

Technical Field

[0001] The present invention relates to the technical field of agricultural production, and in particular to a crop drought resistance agent and a method for improving crop drought resistance and / or promoting crop growth and development and applications thereof. Background Art

[0002] Drought refers to the phenomenon that when the water consumption of plants is greater than the water absorption, resulting in water deficit in the body. The mechanical damage to plant cells is an important cause of plant death. When plants are subjected to drought stress, the water balance in the body is destroyed, and young and tender tissues take water from old leaves, flowers and fruits, causing the old leaves to wilt and reduce yields; the content of plant hormones also changes accordingly, with an increase in the content of abscisic acid (ABA) and ethylene (ETH), and a decrease in the content of auxin (IAA), gibberellin (GA) and cytokinin (CTK). Studies have shown that under drought stress, the intracellular membrane system is damaged, membrane permeability is lost, inorganic ions and small organic molecules leak out of the tissue, the normal physiological metabolism of cells is destroyed, and protein synthesis is blocked. Drought can also disrupt respiration, produce a large amount of reactive oxygen species (ROS), affect CO2 absorption, weaken the Hill reaction and photosynthetic phosphorylation, and thus hinder photosynthesis.

[0003] Drought stress is one of the important factors affecting agricultural production. In arid and semi-arid areas, the water use efficiency and drought resistance of crops determine the yield and quality of crops. Existing technologies usually use improved field management techniques or the application of chemical agricultural preparations to deal with drought stress. However, chemical agricultural preparations are difficult to degrade, easy to leave residues, and have unstable drought resistance effects, which will also have a certain impact on plant photosynthesis. Excessive application of chemical agricultural preparations has exposed problems such as low effective utilization rate, serious environmental pollution, and high food safety risks, which has had a great negative impact on agricultural production and the ecological environment. Summary of the invention

[0004] The object of the present invention is to provide a crop drought-resistant agent and a method for improving crop drought resistance and / or promoting crop growth and development and application thereof, so as to solve the problems of poor drought-resistant effect and stability, low effective utilization rate, easy residue and high safety risk of agricultural drought-resistant preparations in the prior art.

[0005] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0006] The invention provides a crop drought resistance agent, comprising the following components: arginine, chitosan, salicylic acid, aminoethyl hexanoate, betaine, brassinolide and water;

[0007] The mass volume ratio of arginine to water is 150-300 mg:1 L;

[0008] The mass volume ratio of chitosan to water is 15-45 mg:1 L;

[0009] The mass-volume ratio of the salicylic acid to water is 170 - 330 mg:1 L;

[0010] The mass-volume ratio of the diethyl aminoethyl hexanoate to water is 100 - 200 mg:1 L;

[0011] The mass-volume ratio of the betaine to water is 40 - 120 mg:1 L;

[0012] The mass-volume ratio of the brassinolide to water is 15 - 75 mg:1 L.

[0013] The present invention also provides an application of the described crop drought-resistant agent in improving the drought resistance of crops and / or promoting the growth and development of crops.

[0014] Preferably, the crops include corn, soybean, sorghum, wheat or flax.

[0015] The present invention also provides a preparation method of the described crop drought-resistant agent, which is to mix arginine, chitosan, salicylic acid, diethyl aminoethyl hexanoate, betaine, brassinolide and water to obtain the crop drought-resistant agent.

[0016] The present invention also provides a method for improving the drought resistance of crops and / or promoting the growth and development of crops, which includes the following steps: starting to spray the crop drought-resistant agent when the crops just enter the seedling stage, and the single spraying amount is 75 - 2400 mL / m 2 ;

[0017] The crop drought-resistant agent is the described crop drought-resistant agent.

[0018] Preferably, when the crops just enter the seedling stage, it means that the first crop plant just enters the seedling stage.

[0019] Preferably, the number of spraying times is 8 - 12 times.

[0020] Preferably, the time interval between sprayings is 2 - 5 d.

[0021] Preferably, the crops include corn, soybean, sorghum, wheat or flax.

[0022] The present invention also provides an application of the described method in improving the drought resistance of crops and / or promoting the growth and development of crops.

[0023] The present invention has the following technical effects and advantages:

[0024] In the crop drought-resistant agent of the present invention, arginine, as an amino acid osmotic adjustment substance, can reduce the osmotic potential of plant cells under drought conditions, enabling the cells to maintain a certain amount of water to sustain normal physiological functions, and can participate in the nitrogen metabolism, nitrogen cycle, and redox reactions of the plant to scavenge ROS; chitosan can regulate the opening and closing of stomata to reduce water loss, enhance the activities of SOD and POD in cells, reduce the degree of membrane lipid peroxidation, and effectively scavenge ROS generated by the plant under drought stress; salicylic acid can regulate the closing of stomata to enhance the water retention ability of the plant, induce the plant to accumulate osmotic adjustment substances such as proline and soluble sugars, and increase the activity of antioxidant enzymes in the crop; DA-6 can promote plant cell division and elongation growth, enhance the photosynthesis of the crop, can also regulate the hormone balance in the crop, increase the activities of various antioxidant enzymes, and participate in the metabolic process to promote the growth and development of the crop and enhance the drought resistance of the crop; DA-6 and salicylic acid also have a synergistic effect in crop drought resistance, can jointly induce the plant to accumulate osmotic adjustment substances such as proline and soluble sugars, improve the photosynthesis efficiency, and strengthen the antioxidant defense system; betaine is a quaternary ammonium base osmotic adjustment substance, which can prompt the cell to absorb and retain water from the external environment, maintain the normal turgor pressure of the cell, and can stabilize the structure and function of the enzyme system and biological membrane system, preventing the spatial structure change of functional proteins and membrane lipid peroxidation caused by drought stress; brassinolide can promote the growth and development of crop roots, improve the root activity and absorption ability, reduce the water loss caused by transpiration, thereby enhancing the drought resistance of the crop;

[0025] The crop drought-resistant agent of the present invention can promote the normal growth and development of crops under drought conditions, increase the accumulation of plant biomass and the activity of cell antioxidant enzymes, reduce the toxicity of ROS to the plant, thereby improving the adaptability, tolerance, and resistance of the crop to drought stress, and has the characteristics of good drought resistance effect, strong stability, and high safety. Description of the Drawings

[0026] Figure 1 It is the determination result of the morphological indexes of corn in different treatment groups under drought conditions. The left figure is the determination result of the plant height of corn in different treatment groups, and the right figure is the determination result of the dry weight of corn in different treatment groups;

[0027] Figure 2 It is the determination result of the morphological indexes of soybean in different treatment groups under drought conditions. The left figure is the determination result of the plant height of soybean in different treatment groups, and the right figure is the determination result of the dry weight of soybean in different treatment groups;

[0028] Figure 3 It is the determination result of the morphological indexes of sorghum in different treatment groups under drought conditions. The left figure is the determination result of the plant height of sorghum in different treatment groups, and the right figure is the determination result of the dry weight of sorghum in different treatment groups;

[0029] Figure 4Measurement results of morphological indexes of wheat in different treatment groups under drought conditions. The left figure shows the plant height measurement results of wheat in different treatment groups, and the right figure shows the dry weight measurement results of wheat in different treatment groups;

[0030] Figure 5 Measurement results of morphological indexes of flax in different treatment groups under drought conditions. The left figure shows the plant height measurement results of flax in different treatment groups, and the right figure shows the dry weight measurement results of flax in different treatment groups. Specific implementation mode

[0031] The present invention provides a crop drought-resistant agent, which comprises the following components: arginine, chitosan, salicylic acid, diethyl aminoethyl hexanoate, betaine, brassinolide and water;

[0032] The mass-volume ratio of arginine to water is 150-300 mg:1 L, preferably 202 mg:1 L;

[0033] The mass-volume ratio of chitosan to water is 15-45 mg:1 L, preferably 30 mg:1 L;

[0034] The mass-volume ratio of salicylic acid to water is 170-330 mg:1 L, preferably 290 mg:1 L;

[0035] The mass-volume ratio of diethyl aminoethyl hexanoate to water is 100-200 mg:1 L, preferably 168 mg:1 L;

[0036] The mass-volume ratio of betaine to water is 40-120 mg:1 L, preferably 75 mg:1 L;

[0037] The mass-volume ratio of brassinolide to water is 15-75 mg:1 L, preferably 35 mg:1 L.

[0038] The present invention also provides the application of the crop drought-resistant agent in improving the drought resistance of crops and / or promoting the growth and development of crops.

[0039] In the present invention, the crops include corn, soybean, sorghum, wheat or flax.

[0040] The present invention also provides a preparation method of the crop drought-resistant agent, which mixes arginine, chitosan, salicylic acid, diethyl aminoethyl hexanoate, betaine, brassinolide and water to obtain the crop drought-resistant agent.

[0041] The present invention also provides a method for improving the drought resistance of crops and / or promoting the growth and development of crops, which comprises the following steps: spraying the crop drought-resistant agent when the crops just enter the seedling stage, and the single spraying amount is 75-2400 mL / m 2 ;

[0042] The crop drought-resistant agent is the crop drought-resistant agent described above.

[0043] In the present invention, the single spraying amount of the crop drought-resistant agent sprayed on corn is 75-160 mL / m 2 , preferably 120 mL / m 2 ;

[0044] The single spraying amount of the crop drought-resistant agent sprayed on soybean is 120-300 mL / m 2 , preferably 240 mL / m 2 ;

[0045] The single spraying amount of the crop drought-resistant agent sprayed on sorghum is 160-400 mL / m 2 , preferably 320 mL / m 2 ;

[0046] The single spraying amount of the crop drought-resistant agent sprayed on wheat is 800-2400 mL / m 2 , preferably 1800 mL / m 2 ;

[0047] The single spraying amount of the crop drought-resistant agent sprayed on flax is 1600-2200 mL / m 2 , preferably 2000 mL / m 2 .

[0048] In the present invention, the planting density of corn is 5-10 plants / m 2 , preferably 8 plants / m 2 ;

[0049] The planting density of soybean is 15-20 plants / m 2 , preferably 16 plants / m 2 ;

[0050] The planting density of sorghum is 9-15 plants / m 2 , preferably 10 plants / m 2 ;

[0051] The planting density of wheat is 100-200 plants / m 2 , preferably 150 plants / m 2 ;

[0052] The planting density of flax is 1200-1500 plants / m 2 , preferably 1400 plants / m 2 .

[0053] In the present invention, the first crop plant just entering the seedling stage is when the crop just enters the seedling stage.

[0054] In the present invention, the number of spraying times is 8-12 times, preferably 10 times.

[0055] In the present invention, the spraying time interval is 2 - 5 days, preferably 3 days.

[0056] In the present invention, the crops include corn, soybean, sorghum, wheat or flax.

[0057] The present invention also provides the application of the described method in improving the drought resistance of crops and / or promoting the growth and development of crops.

[0058] The technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0059] Among the tested crops of the present invention, the corn is Hanyu 5, purchased from the Crop Science Research Institute of Shanxi Academy of Agricultural Sciences; the soybean is Jihuang 13, purchased from Hebei Hengyan Seed Industry Co., Ltd.; the sorghum is Hongyingzi, purchased from the Dryland Research Institute of Guizhou Academy of Agricultural Sciences; the wheat is Jimai 22, purchased from the Crop Research Institute of Shandong Academy of Agricultural Sciences; the flax is Longya 8, purchased from the Crop Research Institute of Gansu Academy of Agricultural Sciences;

[0060] Among the reagents of the present invention, Hoagland's nutrient solution is purchased from Beijing Coolaber Technology Co., Ltd.; the plant superoxide dismutase (SOD) kit, plant peroxidase (POD) detection kit, and plant malondialdehyde (MDA) detection kit are purchased from Shanghai Enzyme-linked Biotechnology Co., Ltd.

[0061] Example 1: Preparation of Crop Drought Resistance Agent

[0062] 202 mg of arginine, 45 mg of chitosan, 170 mg of salicylic acid, 168 mg of diethyl aminoethyl hexanoate, 120 mg of betaine, 75 mg of brassinolide and 1 L of water are mixed evenly to obtain the crop drought resistance agent.

[0063] Example 2: Preparation of Crop Drought Resistance Agent

[0064] 300 mg of arginine, 30 mg of chitosan, 290 mg of salicylic acid, 200 mg of diethyl aminoethyl hexanoate, 40 mg of betaine, 35 mg of brassinolide and 1 L of water are mixed evenly to obtain the crop drought resistance agent.

[0065] Example 3: Preparation of Crop Drought Resistance Agent

[0066] 150 mg of arginine, 15 mg of chitosan, 330 mg of salicylic acid, 100 mg of diethyl aminoethyl hexanoate, 75 mg of betaine, 15 mg of brassinolide and 1 L of water are mixed evenly to obtain the crop drought resistance agent.

[0067] Experimental Example 1: Verification of Drought Resistance of Corn under Drought Conditions

[0068] Select 300 seeds of Han Yu No. 5 corn. After germination, transfer them to Hoagland's nutrient solution for cultivation to obtain corn seedlings. When the corn seedlings just enter the seedling stage, select 100 corn seedlings with consistent growth and divide them into two groups, namely the treatment group and the control group, with 50 corn seedlings in each group. Then transfer all of them to Hoagland's nutrient solution containing 15% PEG-6000 for cultivation, and the cultivation density is 8 plants / m 2 , and at the same time, spray the crop drought-resistant agent prepared in Example 1 on the corn seedlings in the treatment group, and spray sterile water on the corn seedlings in the control group. Then spray once every 3 days, with a single spraying amount of 120 mL / m 2 , and spray a total of 8 times. After the treatment, transplant the corn seedlings in each group to the soil and irrigate them with Hoagland's nutrient solution containing 15% PEG-6000. After cultivating for 60 days, measure the plant height and dry weight of the corn plants in each group. Use the plant superoxide dismutase (SOD) kit, plant peroxidase (POD) detection kit, and plant malondialdehyde (MDA) detection kit to measure the SOD, POD activities and MDA content of the corn leaves respectively. The results are shown in Table 1 and Figure 1 as follows.

[0069] Table 1 Antioxidant indexes of corn leaves

[0070] Group SOD Activity (U / gFW) POD Activity (U / gFW) MDA Content (μmol / gFW) Treatment Group 98.13±3.16 68.41±2.75 3.05±0.88 Control Group 76.44±8.25 64.00±3.21 5.93±1.12

[0071] The results show that the average plant height and average dry weight of the corn in the treatment group are 80.55 cm and 2.32 g respectively, which are increased by 12.53% and 25.41% respectively compared with the corn in the control group. The SOD activity of the corn in the treatment group is significantly increased compared with that in the control group, and the MDA content is significantly decreased. It shows that the crop drought-resistant agent of the present invention can significantly promote the growth and biomass accumulation of corn plants under drought conditions, improve the activity of cellular antioxidant enzymes and reduce the toxicity of ROS to plants, thereby enhancing the resistance of corn plants to drought stress.

[0072] Experimental Example 2: Verification of drought resistance of soybeans under drought conditions

[0073] Select 250 seeds of Jihuang 13 soybeans. After germination, transfer them to Hoagland's nutrient solution for cultivation to obtain soybean seedlings. When the soybean seedlings just enter the seedling stage, select 100 soybean seedlings with consistent growth and divide them into two groups, namely the treatment group and the control group, with 50 soybean seedlings in each group. Then transfer all of them to Hoagland's nutrient solution containing 20% PEG-6000 for cultivation, and the cultivation density is 16 plants / m 2 , and at the same time, spray the crop drought-resistant agent prepared in Example 2 on the soybean seedlings in the treatment group, and spray sterile water on the soybean seedlings in the control group. Then spray once every 5 days, with a single spraying amount of 240 mL / m 2, sprayed a total of 8 times. After the treatment, the soybean seedlings in each group were transplanted into the soil and watered with Hoagland's nutrient solution containing 20% PEG-6000. After culturing for 45 days, according to the method described in Experimental Example 1, the plant height, dry weight, SOD, POD activities and MDA content of the soybean plants in each group were measured. The results are shown in Table 2 and Figure 2 as follows.

[0074] Table 2 Antioxidant indexes of soybean leaves

[0075] Group SOD Activity (U / gFW) POD Activity (U / gFW) MDA Content (μmol / gFW) Treatment Group 61.79±1.56 78.26±1.30 21.62±0.85 Control Group 52.15±1.87 57.33±1.45 33.07±0.42

[0076] The results showed that the average plant height and average dry weight of the soybean plants in the treatment group were 65.23 cm and 0.61 g respectively, which were increased by 8.52% and 32.61% compared with those of the control group; the SOD and POD activities of the soybean plants in the treatment group were significantly higher than those of the control group, and the MDA content was significantly lower. It shows that the crop drought-resistant agent of the present invention can significantly improve the biomass accumulation and cell antioxidant enzyme activity of soybean plants under drought conditions, reduce the toxicity of ROS to plants, and thus enhance the tolerance of soybean plants to drought environment.

[0077] Experimental Example 3: Verification of drought resistance of sorghum under drought conditions

[0078] Select 200 Hongyingzi sorghum seeds. After germination, transfer them to Hoagland's nutrient solution for cultivation to obtain sorghum seedlings; when the sorghum seedlings just enter the seedling stage, select 80 sorghum seedlings with consistent growth and divide them into two groups, namely the treatment group and the control group, with 40 sorghum seedlings in each group, and transfer all of them to Hoagland's nutrient solution containing 15% PEG-6000 for cultivation. The cultivation density is 10 plants / m 2 , and at the same time spray the crop drought-resistant agent prepared in Example 3 on the sorghum seedlings in the treatment group, spray sterile water on the sorghum seedlings in the control group, and then spray once every 3 days, with a single spraying amount of 320 mL / m 2 , sprayed a total of 10 times. After the treatment, the sorghum seedlings in each group were transplanted into the soil and watered with Hoagland's nutrient solution containing 15% PEG-6000. After culturing for 50 days, according to the method described in Experimental Example 1, the plant height and dry weight of the sorghum plants in each group were measured. The results are as Figure 3 follows.

[0079] The results showed that the average plant height and average dry weight of the sorghum plants in the treatment group were 68.34 cm and 0.41 g respectively, which were increased by 10.39% and 13.89% compared with those of the control group. It shows that the crop drought-resistant agent of the present invention can promote the growth and biomass accumulation of sorghum plants under drought conditions and enhance the adaptability of sorghum plants to drought environment.

[0080] Experimental Example 4: Verification of drought resistance of wheat under drought conditions

[0081] Select 600 seeds of Jimai 22 wheat. After germination, transfer them to Hoagland's nutrient solution for cultivation to obtain wheat seedlings. When the wheat seedlings just enter the seedling stage, select 400 wheat seedlings with consistent growth and divide them into two groups, namely the treatment group and the control group, with 200 wheat seedlings in each group. Then transfer all of them to Hoagland's nutrient solution containing 20% PEG-6000 for cultivation, and the cultivation density is 150 plants / m 2 , and at the same time, spray the crop drought-resistant agent prepared in Example 1 on the wheat seedlings in the treatment group, and spray sterile water on the wheat seedlings in the control group. Then spray once every 4 days, and the single spraying amount is 1800 mL / m 2 , and spray a total of 12 times. After the treatment is completed, transplant the wheat seedlings in each group to the soil and irrigate them with Hoagland's nutrient solution containing 20% PEG-6000. After culturing for 65 days, measure the plant height, dry weight, SOD, POD activities and MDA content of the wheat plants in each group according to the method described in Experimental Example 1. The results are shown in Table 3 and Figure 4 as follows.

[0082] Table 3 Antioxidant indexes of wheat leaves

[0083]

[0084]

[0085] The results show that the average plant height and average dry weight of the wheat in the treatment group are 43.40 cm and 0.37 g respectively, which are increased by 16.20% and 15.63% respectively compared with the wheat in the control group; the SOD and POD activities of the wheat in the treatment group are significantly increased compared with the wheat in the control group, and the MDA content is significantly decreased. It shows that the crop drought-resistant agent of the present invention can promote the growth of wheat plants under drought conditions, significantly improve the biomass accumulation and the activity of cellular antioxidant enzymes, reduce the toxicity of ROS to the plants, and thus improve the resistance of wheat plants to drought stress.

[0086] Experimental Example 5: Verification of the drought resistance of flax under drought conditions

[0087] Select 4000 seeds of Longya 8 flax. After germination, transfer them to Hoagland's nutrient solution for cultivation to obtain flax seedlings. When the flax seedlings just enter the seedling stage, select 2800 flax seedlings with consistent growth and divide them into two groups, namely the treatment group and the control group, with 1400 flax seedlings in each group. Then transfer all of them to Hoagland's nutrient solution containing 25% PEG-6000 for cultivation, and the cultivation density is 1400 plants / m 2 , and at the same time, spray the crop drought-resistant agent prepared in Example 3 on the flax seedlings in the treatment group, and spray sterile water on the flax seedlings in the control group. Then spray once every 5 days, and the single spraying amount is 2000 mL / m2 , a total of 8 sprays were carried out. After the treatment was completed, the flax seedlings in each group were transplanted into the soil and irrigated with Hoagland's nutrient solution containing 25% PEG-6000. After culturing for 45 days, according to the method described in Experimental Example 1, the plant height and dry weight of the flax plants in each group were measured. The results are shown in Table 4 and Figure 5 as follows.

[0088] Table 4 Antioxidant indexes of flax leaves

[0089] Group SOD Activity (U / gFW) POD Activity (U / gFW) MDA Content (μmol / gFW) Treatment Group 134.95±2.10 83.64±2.13 2.09±1.15 Control Group 98.27±2.35 77.05±1.92 3.36±1.22

[0090] The results showed that the average plant height and average dry weight of the treated flax were 34.63 cm and 0.38 g respectively, which were increased by 16.44% and 11.76% compared with the control group of flax; the SOD activity of the treated flax was significantly increased compared with that of the control group of flax, and the MDA content was significantly decreased. It shows that the crop drought-resistant agent of the present invention can promote the growth and biomass accumulation of flax plants under drought conditions, improve the activity of cell antioxidant enzymes, reduce the toxicity of ROS to plants, and thus enhance the tolerance and survival ability of flax plants in drought environments.

[0091] As can be seen from the above examples, the present invention provides a crop drought-resistant agent and a method for improving crop drought resistance and / or promoting crop growth and development and its application. The crop drought-resistant agent of the present invention can promote the normal growth and development of crops under drought conditions, improve the biomass accumulation of plants and the activity of cell antioxidant enzymes, reduce the toxicity of ROS to plants, and thus improve the adaptability, tolerance and resistance of crops to drought stress.

[0092] The above is only the preferred embodiment of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and modifications can be made, and these improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. A crop drought-resistant agent, characterized in that, It comprises the following components: arginine, chitosan, salicylic acid, diethyl aminoethyl hexanoate, betaine, brassinolide and water; The mass-volume ratio of the arginine to the water is 150-300 mg:1 L; The mass-volume ratio of the chitosan to the water is 15-45 mg:1 L; The mass-volume ratio of the salicylic acid to the water is 170-330 mg:1 L; The mass-volume ratio of the diethyl aminoethyl hexanoate to the water is 100-200 mg:1 L; The mass-volume ratio of the betaine to the water is 40-120 mg:1 L; The mass-volume ratio of the brassinolide to the water is 15-75 mg:1 L.

2. Use of the crop drought-resistant agent according to claim 1 in improving the drought resistance of crops and / or promoting the growth and development of crops.

3. The application according to claim 2, characterized in that The crops include maize, soybean, sorghum, wheat or flax.

4. The preparation method of the crop drought-resistant agent according to claim 1, characterized in that, Arginine, chitosan, salicylic acid, diethyl aminoethyl hexanoate, betaine, brassinolide and water are mixed to obtain the crop drought-resistant agent.

5. A method for improving the drought resistance of crops and / or promoting the growth and development of crops, characterized in that, It includes the following steps: When the crop just enters the seedling stage, start spraying the crop drought-resistant agent, and the single spraying amount is 75-2400 mL / m 2 ; The crop drought-resistant agent is the crop drought-resistant agent according to claim 1.

6. The method according to claim 5, wherein When the crop just enters the seedling stage, it means the first crop plant just enters the seedling stage.

7. The method according to claim 6, wherein The number of spraying times is 8-12 times.

8. The method according to claim 7, characterized in that The time interval between sprayings is 2-5 d.

9. The method according to claim 8, wherein The crops include maize, soybean, sorghum, wheat or flax.

10. Use of the method according to any one of claims 5-9 in improving the drought resistance of crops and / or promoting the growth and development of crops.

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