Identification and application of cotton bHLH transcription factor gene GhFP1 and promoter thereof

A promoter sequence, cotton technology, applied in application, genetic engineering, plant gene improvement and other directions, to achieve the effect of high specific expression activity

Active Publication Date: 2015-06-17
HUAZHONG NORMAL UNIV
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Problems solved by technology

Although there are no reports on the function of HLH / bHLH transcription factors in cotton fiber development, more than 200 HLH / bHLH family transcription factor members were found in the genome sequencing of diploid cotton, implying that HLH / bHLH transcription factors May play an important role in cotton fiber cell development

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  • Identification and application of cotton bHLH transcription factor gene GhFP1 and promoter thereof
  • Identification and application of cotton bHLH transcription factor gene GhFP1 and promoter thereof
  • Identification and application of cotton bHLH transcription factor gene GhFP1 and promoter thereof

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Embodiment Construction

[0031] The present invention is further illustrated by the following figures and implementations, but does not limit the scope of the present invention.

[0032] 1. Isolation and cloning of full-length cDNA of cotton GhFP1 gene

[0033] A new gene of the bHLH family was isolated from the cotton fiber cDNA library (according to Li XB et al., 2002, Molecular characterization of the cotton GhTUB1gene that is preferentially expressed in fiber.Plant Physiol 130:666-674), named GhFP1 . Analysis shows that the full-length sequence of GhFP1 cDNA is 1262bp, as shown in SEQ.ID.No.1, and the open reading frame is 1068bp, encoding a bHLH protein containing 355 amino acids, and the amino acid sequence of the protein is shown in SEQ.ID.No.2, The molecular weight is 39.2 kDa.

[0034] 2. Isolation and cloning of GhFP1 promoter sequence and GUS staining analysis

[0035] A GhFP1 promoter fragment with a length of 1061 bp was isolated and cloned by chromosome walking method, and the sequenc...

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Abstract

The invention relates to a cotton GhFP1 gene which belongs to a member of a bHLH transcription factor gene family, the cDNA (complementary deoxyribonucleic acid) full length is 1262bp, and the cotton GhFP1 gene can code a bHLH protein containing 355 amino acids. GhFP1 is respectively expressed in various tissues of cotton, and is highly expressed in the fiber development premetaphase. The GhFP1 promoter segment with the length of 1061bp is separated and cloned. The analysis on the activity of the promoter by using the GUS report gene indicates that the promoter has higher specific expression activity in cotton fibers. The experiment indicates that the overexpression of GhFP1 in Arabidopsis thaliana results in increase of leaf epidermal hair; and the overexpression of GhFP1 in cotton results in enhancement of expression of related genes of cotton fibrocyte brassinolide and increase of ripe cotton fiber length. The result proves that the GhFP1 protein performs positive regulating actions and promotes elongation of fibrocytes in the cotton fiber elongation process, and thus, possibly has important application value in improving the cotton fiber quality and increasing the cotton yield.

Description

technical field [0001] The invention relates to cotton genes, specifically the expression and functional identification of a new member gene GhFP1 in the cotton basic helix-loop-helix (bHLH) family, and the identification of the fiber-specific expression activity of its promoter, which is further applied to the improvement of cotton fiber quality. Background technique [0002] Cotton is one of the most important economic crops in the world. According to its genome composition, it can be divided into allotetraploid cotton (AADD), and diploid cotton (AA) and (DD). The cotton currently planted is mainly allotetraploid cotton (accounting for more than 95% of the total cotton planting area), and diploid cotton is less planted. Allotetraploid cotton is divided into upland cotton (Gossypium hirsutum) and sea island cotton (G. barbadense). Upland cotton is originally produced in Central America and has wide adaptability. It is currently the most planted cotton species, accounting ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/113C12Q1/68
Inventor 李学宝刘致浩陈云
Owner HUAZHONG NORMAL UNIV
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