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Identification and application of a cotton bhlh transcription factor gene ghfp1 and its promoter

A promoter and cotton technology, applied in the fields of application, genetic engineering, plant gene improvement, etc., to achieve the effect of high specific expression activity

Active Publication Date: 2018-04-27
HUAZHONG NORMAL UNIV
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Problems solved by technology

Although there are no reports on the function of HLH / bHLH transcription factors in cotton fiber development, more than 200 HLH / bHLH family transcription factor members were found in the genome sequencing of diploid cotton, implying that HLH / bHLH transcription factors May play an important role in cotton fiber cell development

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  • Identification and application of a cotton bhlh transcription factor gene ghfp1 and its promoter
  • Identification and application of a cotton bhlh transcription factor gene ghfp1 and its promoter
  • Identification and application of a cotton bhlh transcription factor gene ghfp1 and its promoter

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Embodiment Construction

[0031] The present invention is further illustrated by the following figures and implementations, but does not limit the scope of the present invention.

[0032] 1. Isolation and cloning of full-length cDNA of cotton GhFP1 gene

[0033] A new gene of the bHLH family was isolated from the cotton fiber cDNA library (according to Li XB et al., 2002, Molecular characterization of the cotton GhTUB1 gene that is preferentially expressed in fiber. Plant Physiol 130: 666-674), named GhFP1. Analysis shows that the full-length sequence of GhFP1 cDNA is 1262bp, as shown in SEQ.ID.No.1, and the open reading frame is 1068bp, encoding a bHLH protein containing 355 amino acids, and the amino acid sequence of the protein is shown in SEQ.ID.No.2, The molecular weight is 39.2 kDa.

[0034] 2. Isolation and cloning of GhFP1 promoter sequence and GUS staining analysis

[0035] A GhFP1 promoter fragment with a length of 1061 bp was isolated and cloned by chromosome walking method, and the sequen...

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Abstract

The cotton GhFP1 gene is a member of the bHLH transcription factor gene family, and its cDNA is 1262bp in full length, encoding a bHLH protein containing 355 amino acids. GhFP1 was expressed in all tissues of cotton, and it was highly expressed in the pre-middle stage of fiber development. The GhFP1 promoter fragment with a length of 1061bp was isolated and cloned. The promoter activity was analyzed by GUS reporter gene, which showed that the promoter had high specific expression activity in cotton fiber. Experiments have shown that overexpression of GhFP1 in Arabidopsis leads to growth of leaf epidermis; overexpression of GhFP1 in cotton leads to up-regulation of brassinolide-related genes in cotton fiber cells and increased length of mature cotton fibers. The above results prove that GhFP1 protein plays a positive regulatory role in the process of cotton fiber elongation and promotes fiber cell elongation, which may have important application value for improving cotton fiber quality and increasing cotton yield.

Description

technical field [0001] The invention relates to cotton genes, specifically the expression and functional identification of a new member gene GhFP1 in the cotton basic helix-loop-helix (bHLH) family, and the identification of the fiber-specific expression activity of its promoter, which is further applied to the improvement of cotton fiber quality. Background technique [0002] Cotton is one of the most important economic crops in the world. According to its genome composition, it can be divided into allotetraploid cotton (AADD), and diploid cotton (AA) and (DD). The cotton currently planted is mainly allotetraploid cotton (accounting for more than 95% of the total cotton planting area), and diploid cotton is less planted. Allotetraploid cotton is divided into upland cotton (Gossypium hirsutum) and sea island cotton (G. barbadense). Upland cotton is originally produced in Central America and has wide adaptability. It is currently the most planted cotton species, accounting ...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/113C12Q1/6895
Inventor 李学宝刘致浩陈云
Owner HUAZHONG NORMAL UNIV
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