15 results about "Fiber elongation" patented technology

The invention discloses an application of a cotton transcription factor GhMYB44A05 in improvement of cotton fiber quality. It is verified for the first time that the transcription factor GhMYB44A05 (GhiA05G026680) is an important gene in the cotton fiber development process, and the transcription factor is dominantly expressed in the cotton fiber elongation and thickening periods and the ovule development later period, so that the quality, such as the length and the strength, of the cotton fiber is affected. Subcellular localization analysis shows that the protein is localized in a cell nucleus and has general characteristics of transcription factors. Transgenic experiments prove that overexpression of the GhMYB44A05 can significantly increase the length and strength of the fibers, and the cultivation and development of high-fiber-quality cotton varieties are promoted.

The invention discloses application of a GhSP1L4D gene in regulation and control of cotton fiber elongation, and belongs to the technical field of gene engineering. The invention discovers that the GhSP1L4D gene presents specific high expression in the fiber development process for the first time, and especially the expression quantity is significant 20 days after flowering (20DPA). Furthermore, experiments prove that the GhSP1L4D gene can influence the helicity of cotton fibers, the thickness of cell walls of the cotton fibers and the content of cellulose and pectin in the cotton fibers so as to regulate and control the length of the cotton fibers, specifically, the expression of the GhSP1L4D gene is inhibited through virus induced gene silencing (VIGS) in the cotton; as a result, the fiber length is shortened, the cell length is shortened, the spiral number of the fiber is increased, the content of cellulose and pectin in the cotton fiber is increased, and new gene resources and theoretical support are provided for quality improvement of the cotton fiber.

The invention relates to the technical field of gene engineering, in particular to application of a cotton GhGELP68D gene in regulation and control of cotton fiber elongation. According to the invention, the GhGELP68D protein belonging to a GDSL family is identified in upland cotton, and the nucleotide sequence of the coding gene GhGELP68D is as shown in SEQ ID NO. 1. A cotton transgenic overexpression plant is obtained through a cotton tissue culture technology, and compared with a wild type cotton fiber, overexpression of GhGELP68D causes length elongation of the cotton fiber; a silent vector is constructed through a VIGS technology, cotton is transformed, and the result shows that compared with an empty vector, the cotton fiber length of a GhGELP68D silent plant is obviously shortened. A key gene resource is provided for cotton fiber quality improvement, remarkable economic benefits are expected to be brought in the field of plant production, and the application prospect is extremely wide.

The invention belongs to the technical field of plant expression vectors and application thereof, and discloses a method for improving fiber length by changing extracellular body acidification degree, which comprises the following steps: fusing a specific promoter with a PM < H + >-ATP enzyme gene to construct a plant expression vector for specifically expressing PM < H + >-ATP enzyme, and integrating the plant expression vector into a cotton genome to obtain the fiber length. According to the method, the pH value of fiber cytoplasm exosome is moderately reduced in the fiber elongation process, the length of the cotton fiber can be remarkably increased, and high-quality fiber is provided for the cotton industry and the textile industry. According to the method for improving the length of the cotton fiber, AHA related genes are specifically expressed in fiber cells of cotton, then the acidification degree of cell walls in the fiber elongation process is regulated and controlled, the cell walls are moderately acidified and relaxed, development of the cotton fiber is controlled, and the purpose of increasing the length of the cotton fiber is achieved. Test results prove that the length of the cotton fibers improved by the method for improving the characters of the cotton fibers is obviously increased.

This invention discloses a WD40-like family protein gene, GoWD40L, for improving cotton fiber quality and its applications, belonging to the field of biotechnology applications. This WD40-like family protein gene, GoWD40L, exists in two haplotypes: haplotype 1 has a CDS sequence of SEQ ID NO.1; and haplotype 2 has a CDS sequence of SEQ ID NO.4. When the haplotype 2 gene sequence or its encoded protein is present in cotton plants, the allele or protein can promote an increase in cotton fiber length, fiber strength, fiber uniformity, and fiber elongation, and a decrease in micronaire value. This indicates that haplotype 2 of this gene plays an important role and has promising applications in improving cotton fiber quality traits and in breeding high-quality new cotton varieties.

The invention provides a method for synergistically regulating cotton fiber elongation and verticillium wilt resistance based on an upland cotton GhMP gene and a transgenic cotton plant, and relates to the technical field of genetic engineering. The gene encodes a membrane protein, and overexpression and RNA interference transgenic line experiments prove that the expression level of GhMP is positively correlated with fiber development and disease resistance: the fiber length can be significantly increased and the verticillium wilt resistance can be improved by expression increase, and the fiber shortening and the resistance weakening can be caused by expression reduction. A further research shows that the GhMP protein is positioned on a cell membrane, can interact with GhPIP2.7 and GhTIP1.1 aquaporin, and jointly participates in hydrogen peroxide transmembrane transport to form a molecular compound for regulating and controlling fiber elongation and preventing diseases. The invention provides a key target gene with both quality improvement and disease resistance enhancement, provides a new strategy and gene resources for cotton multi-trait molecular breeding, and has important application value and popularization prospect.

The invention provides an application of a GhMAKR1 gene of upland cotton in regulation and control of cotton fiber elongation and plant morphology, 419 parts and 1081 parts of upland cotton varieties are re-sequenced and subjected to whole genome association analysis with fiber length traits, and it is found that the GhMAKR1 gene located in a D11 chromosome has significantly associated SNP and SV with the cotton fiber length. Gene overexpression and gene silencing means are used for verification and show that high expression of GhMAKR1 in a fiber development stage can significantly inhibit fiber elongation, and silencing causes fiber length increase. Meanwhile, research finds that the GhMAKR1 is used as an unknown functional protein, and overexpression of the GhMAKR1 shows a series of unique phenotypic characteristics, including dwarfing which is mainly shown in stem and internode shortening. In addition, overexpression plants also produce short and small flowers, the vigor of the flowers is reduced, and cotton bolls and seeds become small. The GhMAKR1 and the GhBIN2 protein can form an interaction compound to jointly inhibit the fiber cell elongation process.

The invention relates to a fibrilia composite modification method with functions of improving fiber elongation at break and endowing ammonia gas with intelligent visual detection. The fibrilia composite modification method comprises the following steps: step 1, soaking fibrilia in a high-concentration zinc chloride solution (the mass fraction of zinc chloride is 63-65%) for modification; step 2, extracting purple cabbage with water to prepare a purple cabbage extracting solution, then putting the fibrilia modified by zinc chloride into the purple cabbage extracting solution, finishing for a period of time at room temperature, taking out and drying to obtain the composite modified fibrilia. According to the modification method, the elongation at break of the jute fibers can be effectively improved, and the jute fibers are endowed with an ammonia gas visual intelligent color-changing indication function.

The invention discloses a flame-retardant polyolefin fiber and a preparation method thereof, and relates to the technical field of fiber preparation, and the flame-retardant polyolefin fiber comprises the following raw materials: polypropylene, modified epoxy fatty acid methyl ester, modified calcium carbonate, modified melamine cyanurate, a light stabilizer 944, an ultraviolet light absorber, an antioxidant and a compatilizer. According to the invention, the modified melamine cyanurate is added, so that the flame retardant property of the fiber can be remarkably improved, no molten drops exist during combustion of the fiber, the fuming amount is greatly reduced, meanwhile, the dispersion uniformity is good, weak spots cannot be formed in the fiber due to agglomeration, the thermal oxidation stability and aging resistance of the fiber are improved, and the fiber is not easy to decompose and lose efficacy in a high-temperature environment; while efficient flame retardance is realized, negative effects on mechanical properties such as elongation at break, toughness and the like of the fiber are reduced to the greatest extent, and flame retardance and practicability are both considered.

This invention provides a method based on upland cotton GhMP This research relates to the field of genetic engineering technology and describes a method for the coordinated regulation of cotton fiber elongation and Verticillium wilt resistance using genes, as well as transgenic cotton plants. The gene encodes a membrane protein, and experiments using overexpression and RNA interference transgenic lines have demonstrated its effectiveness. GhMP The expression level of this compound is positively correlated with fiber development and disease resistance: increased expression significantly increases fiber length and improves resistance to Verticillium wilt, while decreased expression leads to fiber shortening and weakened resistance. Further studies have shown that... GhMP Proteins are located on the cell membrane and can interact with... GhPIP2.7 and GhTIP1.1 Aquaporins interact and jointly participate in the transmembrane transport of hydrogen peroxide, forming a molecular complex that regulates fiber elongation and disease resistance. This invention provides a key target gene that combines quality improvement and disease resistance enhancement, offering a new strategy and gene resource for multi-trait molecular breeding of cotton, and has significant application value and promising prospects.

The invention provides an application of a cotton enoyl coenzyme A reductase gene GhECRA10 in improvement of cotton fiber quality. The cotton enoyl coenzyme A reductase gene GhECRA10 is predominantly expressed in the early stage of cotton fiber elongation, but the expression quantity is gradually reduced along with fiber development in the later stage. The gene is knocked out by using a gene editing system to create a mutant for functional verification, and the result shows that the mutated GhECRA10 gene can significantly increase the fiber length and strength, significantly reduce the micronaire value (A-grade high-quality fiber), enlarge seeds and increase the hundred-grain weight of the seeds, thereby providing a new way and means for improving the quality and yield of cotton and having broad application prospects. And the method has extremely high application value and potential.

The present invention discloses a bio-based polymer melt-blended functional fiber and a preparation method thereof, wherein a liquid crystal elastomer is added to the melt spinning process of the bio-based polymer polylactic acid fiber for blending and melt spinning to improve the physical and mechanical properties of the fiber and form a functional fiber. Specifically, polylactic acid chips are mixed with a liquid crystal elastomer, and then the liquid crystal-polylactic acid blended fiber is prepared according to a melt spinning process. By regulating specific process parameters, liquid crystal-polylactic acid fibers can be successfully spun, and the prepared liquid crystal-polylactic acid blended fibers have excellent physical and mechanical properties, with an average fiber strength of up to 2.54 cN / dtex and an average elongation of 110.24%; at the same time, they also have the special properties of liquid crystal elastomers. The present invention can also directly produce liquid-dyed fibers with excellent performance, with an average fiber elongation of 196.02% and obvious temperature response characteristics. The method of the present invention is simple, can be mass-produced, and is a degradable and environmentally friendly fiber with extremely high application prospects.

The invention belongs to the technical field of application of plant growth regulators, and particularly relates to application of 4-HPEA in regulation and control of cotton fiber length. According to the application disclosed by the invention, wide-target metabolome and targeted metabolite detection is carried out on GhADH1 transgenic cotton fiber tissues, the fact that accumulation of 4-HPEA (4-hydroxyphenethyl alcohol) in fibers is positively regulated by GhADH1 is found, and the fact that 4-HPEA is a potential catalytic product of GhADH1 is preliminarily determined. In-vitro enzyme activity and ovule in-vitro culture experiments are further combined to prove that the 4-HPEA is a direct catalytic product of GhADH1, and the 4-HPEA can obviously promote the elongation of fibers under a proper concentration condition. As a novel growth regulator for promoting fiber elongation, the 4-HPEA can provide a new regulation direction and an improved idea for molecular breeding of long-fiber cotton.

The invention discloses application of GhKCR1 protein in regulation and control of cotton fiber quality, and relates to the technical field of cotton fiber length regulation and control. A knockout mutant CR-GhKCR1 of the GhKCR1 gene of the upland cotton is constructed by utilizing a CRISPR / Cas9 technology, the strain is compared with a wild type, the cotton of the CR-GhKCR1 strain is found to have remarkable advantages in the aspects of fiber length, cotton boll size, quantity and the like, the GhKCR1 gene is speculated to possibly participate in the regulation and control process of fiber elongation, and the GhKCR1 gene can be used for preparing the GhKCR1 gene of the upland cotton. The knockout can significantly improve the yield and quality of cotton fibers, which provides a theoretical basis for the cultivation of long-fiber cotton and is hopeful to cultivate high-yield cotton.

The invention provides application of an upland cotton GhFAM32Al gene in regulation and control of cotton fiber elongation, and relates to the technical field of genetic engineering. According to the invention, a recombinant inbred line group is constructed among parents with significant difference in fiber length and QTL positioning is carried out, so that the GhFAM32Al gene located in the D02 chromosome has significant influence on the cotton fiber length. Gene overexpression and CRISPR / Cas9 knockout means are used for verification and show that high expression of GhFAM32Al in the fiber development stage can remarkably promote fiber elongation, and fiber length reduction is caused by knockout. Further research finds that GhFAM32Al is a nuclear localized natural disordered protein, can form an interaction compound with GhMYBd protein, and synergistically participates in the regulation and control of the fiber cell elongation process. The invention provides a key gene resource and a molecular regulation strategy for high-quality breeding of cotton.