12 results about "Fibre cell" patented technology

A method for edge passivation of crystalline silicon-based shingle cells, in which multiple crystalline silicon-based shingle cells are stacked to form a shingle cell group with cut edges thereof co-planar to form a to-be-passivated surface, and then the shingle cell group is placed in a fixing tooling to form a passivation unit. The passivation unit is conveyed by a conveying mechanism to an evaporation and annealing mechanism. A first source material is evaporated at 20-300° C. under vacuum in the evaporation and annealing mechanism to form a first gaseous source material, which is deposited on the to-be-passivated surface to form a first passivation layer. The shingle cell group with the first passivation layer is annealed at 100-350° C. A passivation device for implementing such method is further provided.

A method of producing T cells using a cell expansion system includes expanding T cells using a small bioreactor of the cell expansion system, the small bioreactor having a surface area of about 2000 cm2 and an in-capillary volume of about 58 ml. The method further includes flowing the T cells into the mini bioreactor of the cell expansion system. The rate at which the T cells flow into the miniature bioreactor may be greater than or equal to about 0.007 [mu] L / min / fiber to less than or equal to about 0.0281 [mu] L / min / fiber. The cell expansion system may also include a set of conduits in fluid communication with the small bioreactor, and the volume ratio of the set of conduits to the bioreactor may be about 2.96.

A method for producing T-cells using a cell expansion system includes expanding T-cells using a small bioreactor of the cell expansion system, the small bioreactor having a surface area of about 2,000 cm2 and an intracapillary volume of about 58 milliliters. The method further includes causing T-cells to flow into a small bioreactor of the cell expansion system. A rate at which the T-cells flow into the small bioreactor may be greater than or equal to about 0.007 μL / min / fiber to less than or equal to about 0.0281 μL / min / fiber. The cell expansion system may further include a tubing set that is in fluid communication with the small bioreactor and a volume ratio of the tubing set to the bioreactor may be about 2.96.

The invention belongs to the technical field of gene engineering, and particularly relates to application of PagGT43B protein in regulating aspen to adapt to nitrogen deficiency. The amino acid sequence of the PagGT43B protein is as shown in SEQ ID NO. 1, and the nucleotide sequence of the coding gene of the PagGT43B protein is as shown in SEQ ID NO. 2. Tests prove that under the low-nitrogen condition, overexpression of the PagGT43B gene can promote biosynthesis of xylan and increase the thickness of fiber cell walls, so that the adaptability of the poplar to the low-nitrogen environment is improved.

This disclosure describes methods and apparatuses for creating or using a fibrous cell substrate that is formed in a mold using temperature and wettability gradients and that upon which cells can be grown. The disclosed method can include utilizing a temperature gradient and a wettability gradient within a mold to form a porous cell substrate. In particular, the temperature and wettability gradients control nucleation and growth of crystals within the cell substrate solution to form parallel layers of cell substrate. The crystals can be sublimated by freeze drying and the porous cell substrate is seeded. More specifically, the disclosed method includes using pressure and an increased cell mixture viscosity to seed cells deep within the cell substrate. This disclosure also describes an apparatus for forming the structured porous cell substrate.

The application belongs to the technical field of genetic engineering, and particularly relates to application of a PagMYB057 protein in regulating nitrogen deficiency adaptation of poplar. The amino acid sequence of the PagMYB057 protein is shown as SEQ ID NO. 1, and the nucleotide sequence of the coding gene is shown as SEQ ID NO. 2. The application proves through experiments that under low nitrogen conditions, PagMYB057 Gene overexpression can promote xylan biosynthesis, increase fiber cell wall thickness, and thus improve the adaptability of silver gland poplar to a low nitrogen environment.

The invention provides a cold-chain compression-resistant corrugated board which sequentially comprises a surface paper layer, a corrugated core layer and an inner paper layer, and the corrugated core layer is composed of a plurality of V-shaped corrugated units periodically arranged at the oblique angle of 70 + / -5 degrees; nano-crystalline cellulose with the particle size of 50-80 nm and modified soybean protein glue are added into paper pulp of the V-shaped ridge units, and the nano-crystalline cellulose penetrates through fiber cell walls to form a three-dimensional enhanced network; a triangular arch support with a vertex angle theta of 75 + / -5 degrees is arranged on the inner side of each V-shaped ridge unit, corrugated interfaces are formed on the two side surfaces, close to two side ridges of the V-shaped ridge units, of the triangular arch supports, the amplitude A of the corrugated interfaces is 0.2-0.5 mm, the corrugated interfaces and the V-shaped ridge units are bonded in a staggered mode through degradable hot melt adhesive, and the bonding strength is larger than or equal to 2.0 N / mm; the outer surface of the inner paper layer is coated with a water-based polyurethane moisture-proof coating with the moisture penetration rate being less than or equal to 5g / m.24h; a cold chain functional layer is adhered and fixed on one side, close to the corrugated core layer, of the surface paper layer; and the cold chain functional layer comprises a paper-based thermal insulation layer and a foamed starch filling layer. The compressive strength, the buffering performance and the cold chain adaptability are improved.

The present invention provides a turbot gill cell line, which has a deposit number of CCTCCNO: C2022230. The turbot gill tissue cell line SMG provided by the present invention can be continuously passaged. Experimental results show that it can be passaged up to 65 generations and remains stable, and can provide a large number of stable turbot gill cells for the study of immune function genes. The provided cell line has excellent properties and is a fibroblast. The cells are spindle-shaped or irregularly triangular, with several irregular protrusions extending outward from the cell body, and the cells are closely arranged. It is passaged once every 3-4 days, has high recovery efficiency, and can be used for plasmid transfection, siRNA interference and other molecular biology experiments.

The application discloses a fibroblast transfection method and system based on automatic parameter optimization, integrates an automatic liquid treatment workstation and matched hardware to construct an automatic device, the matched hardware comprises an electrotransfection module, a culture box and a cell counter, key parameter groups of transfection are screened to form a characteristic vector, and a value range and a constraint condition are set; subsequently, cells are pretreated and data is collected, standard data is obtained through missing value filling and outlier elimination; then, a random forest model is used to screen core optimization parameters, and a particle swarm optimization algorithm is used to obtain an optimal parameter combination; finally, the optimal parameters are analyzed into hardware instructions for execution. The system comprises a server, the automatic liquid treatment workstation and the matched hardware. The method realizes automatic transfection and intelligent parameter optimization of a whole process, and improves transfection efficiency and stability.

The invention belongs to the technical field of gene engineering, and particularly relates to application of PagMYB057 protein in regulating aspen to adapt to nitrogen deficiency. The amino acid sequence of the PagMYB057 protein is as shown in SEQ ID NO. 1, and the nucleotide sequence of the coding gene of the PagMYB057 protein is as shown in SEQ ID NO. 2. Tests prove that under the low-nitrogen condition, overexpression of the PagMYB057 gene can promote biosynthesis of xylan and increase the thickness of fiber cell walls, so that the adaptability of the poplar to the low-nitrogen environment is improved.

The invention relates to a method for preparing iPSC and differentiating the iPSC into iNKT cells. The method comprises the following steps: (1) introducing a reprogramming induction factor into the iNKT cells, and then reprogramming; (2) culturing the reprogrammed cells to obtain iPSCs, in which the culturing process comprises: (a) culturing the reprogrammed cells in a culture medium comprising basic fibroblast growth factor (bFGF), and (b) culturing the reprogrammed cells in a culture medium comprising an antioxidant, a glycogen synthase kinase 3 (GSK-3) inhibitor, a ROCK inhibitor, and a histone deacetylase (HDAC) inhibitor; and (3) differentiating the iPSCs into the iNKT cells.