7 results about "Fibre cell" patented technology

The invention belongs to the technical field of gene engineering, and particularly relates to application of PagGT43B protein in regulating aspen to adapt to nitrogen deficiency. The amino acid sequence of the PagGT43B protein is as shown in SEQ ID NO. 1, and the nucleotide sequence of the coding gene of the PagGT43B protein is as shown in SEQ ID NO. 2. Tests prove that under the low-nitrogen condition, overexpression of the PagGT43B gene can promote biosynthesis of xylan and increase the thickness of fiber cell walls, so that the adaptability of the poplar to the low-nitrogen environment is improved.

The application belongs to the technical field of genetic engineering, and particularly relates to application of a PagMYB057 protein in regulating nitrogen deficiency adaptation of poplar. The amino acid sequence of the PagMYB057 protein is shown as SEQ ID NO. 1, and the nucleotide sequence of the coding gene is shown as SEQ ID NO. 2. The application proves through experiments that under low nitrogen conditions, PagMYB057 Gene overexpression can promote xylan biosynthesis, increase fiber cell wall thickness, and thus improve the adaptability of silver gland poplar to a low nitrogen environment.

The invention provides a cold-chain compression-resistant corrugated board which sequentially comprises a surface paper layer, a corrugated core layer and an inner paper layer, and the corrugated core layer is composed of a plurality of V-shaped corrugated units periodically arranged at the oblique angle of 70 + / -5 degrees; nano-crystalline cellulose with the particle size of 50-80 nm and modified soybean protein glue are added into paper pulp of the V-shaped ridge units, and the nano-crystalline cellulose penetrates through fiber cell walls to form a three-dimensional enhanced network; a triangular arch support with a vertex angle theta of 75 + / -5 degrees is arranged on the inner side of each V-shaped ridge unit, corrugated interfaces are formed on the two side surfaces, close to two side ridges of the V-shaped ridge units, of the triangular arch supports, the amplitude A of the corrugated interfaces is 0.2-0.5 mm, the corrugated interfaces and the V-shaped ridge units are bonded in a staggered mode through degradable hot melt adhesive, and the bonding strength is larger than or equal to 2.0 N / mm; the outer surface of the inner paper layer is coated with a water-based polyurethane moisture-proof coating with the moisture penetration rate being less than or equal to 5g / m.24h; a cold chain functional layer is adhered and fixed on one side, close to the corrugated core layer, of the surface paper layer; and the cold chain functional layer comprises a paper-based thermal insulation layer and a foamed starch filling layer. The compressive strength, the buffering performance and the cold chain adaptability are improved.

The application discloses a fibroblast transfection method and system based on automatic parameter optimization, integrates an automatic liquid treatment workstation and matched hardware to construct an automatic device, the matched hardware comprises an electrotransfection module, a culture box and a cell counter, key parameter groups of transfection are screened to form a characteristic vector, and a value range and a constraint condition are set; subsequently, cells are pretreated and data is collected, standard data is obtained through missing value filling and outlier elimination; then, a random forest model is used to screen core optimization parameters, and a particle swarm optimization algorithm is used to obtain an optimal parameter combination; finally, the optimal parameters are analyzed into hardware instructions for execution. The system comprises a server, the automatic liquid treatment workstation and the matched hardware. The method realizes automatic transfection and intelligent parameter optimization of a whole process, and improves transfection efficiency and stability.

The invention belongs to the technical field of gene engineering, and particularly relates to application of PagMYB057 protein in regulating aspen to adapt to nitrogen deficiency. The amino acid sequence of the PagMYB057 protein is as shown in SEQ ID NO. 1, and the nucleotide sequence of the coding gene of the PagMYB057 protein is as shown in SEQ ID NO. 2. Tests prove that under the low-nitrogen condition, overexpression of the PagMYB057 gene can promote biosynthesis of xylan and increase the thickness of fiber cell walls, so that the adaptability of the poplar to the low-nitrogen environment is improved.

The invention relates to a method for preparing iPSC and differentiating the iPSC into iNKT cells. The method comprises the following steps: (1) introducing a reprogramming induction factor into the iNKT cells, and then reprogramming; (2) culturing the reprogrammed cells to obtain iPSCs, in which the culturing process comprises: (a) culturing the reprogrammed cells in a culture medium comprising basic fibroblast growth factor (bFGF), and (b) culturing the reprogrammed cells in a culture medium comprising an antioxidant, a glycogen synthase kinase 3 (GSK-3) inhibitor, a ROCK inhibitor, and a histone deacetylase (HDAC) inhibitor; and (3) differentiating the iPSCs into the iNKT cells.