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GhJMJ12 gene SNP marker related to cotton fiber quality, and application of same

A cotton fiber and quality technology, applied in the field of GhJMJ12 gene SNP markers, can solve the problem of low resolution of genetic maps, achieve the effects of shortening the cultivation period, increasing fiber length, and improving selection efficiency and accuracy

Active Publication Date: 2020-06-02
HENAN AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the limitation of the number of markers and parental groups, the resolution of its genetic map is low, and it cannot be directly applied to molecular marker-assisted selection of fiber quality traits.

Method used

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  • GhJMJ12 gene SNP marker related to cotton fiber quality, and application of same
  • GhJMJ12 gene SNP marker related to cotton fiber quality, and application of same
  • GhJMJ12 gene SNP marker related to cotton fiber quality, and application of same

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Experimental program
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Effect test

Embodiment 1

[0042] 1. Test materials and methods

[0043] 1. Cotton material and field planting

[0044] The 276 materials used in the present invention come from the cotton varieties that have played important roles in different historical stages in the National Cotton Germplasm Medium-term Bank of the Cotton Research Institute of the Chinese Academy of Agricultural Sciences and the long-term breeding basic materials accumulated by our research team. These materials were planted in Anyang City, Henan Province (2016 and 2017), Jingzhou City, Hubei Province (2016 and 2017), Alar City, Xinjiang Province (2016 and 2017), Jiujiang City, Jiangxi Province (2016), Hubei Province Huanggang City, Province (2017). Planting in random blocks, two replicates in total, with a row length of 6 meters and a row width of 0.8 meters, with 20-25 plants in each row.

[0045] 2. Field phenotype investigation and statistical analysis

[0046] During the blooming period, 10 plants with the same growth in the ...

Embodiment 2

[0068] 1. Identification of key genes

[0069] Linkage disequilibrium block analysis showed that there were two linkage blocks in this interval, and further analysis found that the associated SNP site was not located in this block ( image 3 ), indicating that the locus is located in a recombination hotspot and does not form a linkage with the surrounding chromosomal segments.

[0070] Further analysis found that the SNP site is located on the gene GhJMJ12 (gene number Gh_D09G2376). The gene has the nucleotide sequence shown in SEQ ID NO: 1, and analysis of the gene structure shows that the gene has 7 exons and 6 introns. The SNP site is a non-synonymous mutation at the 7215bp position of the gene in the seventh exon, which is mutated from an A base to a C base, causing the amino acid to change from aspartic acid to alanine ( Figure 4 ).

[0071] The two genotypes (AA type and CC type) formed by this SNP locus were significantly associated with the phenotypes of four fiber...

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Abstract

The invention relates to the technical field of quality breeding of cotton fibers and specifically relates to a GhJMJ12 gene SNP marker related to cotton fiber quality and application of the same. TheGhJMJ12 gene SNP marker related to cotton fiber quality is located at the 7215bp locus of a GhJMJ12 gene of a Dt09 chromosome of cotton, the SNP marker is a base A or C, and the GhJMJ12 gene has a nucleotide sequence shown by SEQ ID NO:1. According to the invention, 10660 SNP markers are used for whole genome association study of quality characters of the cotton fibers, and the study indicates one SNP locus on the GhJMJ12 is significantly correlated with fiber quality characters such as the fiber length, the fiber specific strength, the fiber elongation and the fiber uniformity.

Description

technical field [0001] The invention relates to the technical field of cotton fiber quality breeding, in particular to a GhJMJ12 gene SNP marker related to cotton fiber quality and its application. Background technique [0002] Cotton is an important fiber crop and an important source of natural fibers in the textile industry. In the past breeding process, breeders often only focused on cotton yield. With the improvement of people's quality of life, cultivating cotton with high fiber quality has become a new breeding goal. [0003] Cotton fiber quality traits mainly include fiber length (FL), fiber specific strength (FS), fiber elongation (FE), fiber uniformity (FU) and micronaire value (FM). In recent years, with the release of cotton genome data, many SNP molecular markers have emerged, and association analysis has been widely used to analyze the genetic basis of complex traits in cotton. Compared with traditional linkage analysis, association analysis can use natural po...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11A01H1/04
CPCC12Q1/6895A01H1/04C12Q2600/156C12Q2600/13
Inventor 刘伟马宗斌朱伟章志强
Owner HENAN AGRICULTURAL UNIVERSITY
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