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Zmnf-ya8 gene snp marker related to maize salt tolerance and its application

A technology of salt tolerance and corn, applied in the direction of DNA/RNA fragments, recombinant DNA technology, microbial measurement/inspection, etc., can solve the problems of complex response mechanism to salt stress and limited excavation work, so as to shorten the cultivation period and save time , low-cost effect

Active Publication Date: 2022-05-27
THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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  • Abstract
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AI Technical Summary

Problems solved by technology

The mechanism of response to salt stress in maize is complex, and the mining of key genes with high value for breeding practice is still very limited

Method used

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  • Zmnf-ya8 gene snp marker related to maize salt tolerance and its application
  • Zmnf-ya8 gene snp marker related to maize salt tolerance and its application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1. Test materials and methods

[0031] 1. Corn material and planting

[0032] The 216 maize materials used in the present invention were from a natural variation population of maize containing a total of 540 materials (Liue et al., 2017). The materials were grown in the cultivation room of the Institute of Biotechnology, Chinese Academy of Agricultural Sciences. 8.0cm×8.0cm culture boxes were used for planting, and each culture box was filled with the same amount of nutrient soil, and 10-15 plants were planted in each box, and each material was repeated three times.

[0033] 2. Salt treatment at seedling stage and investigation of survival rate

[0034] Three weeks after sowing, when the seedlings grow to the stage of two leaves and one heart, they are irrigated with 200 mM NaCl, 80 mL per box, once a week for a total of 4 times, and the survival rate of the seedlings is counted in the fifth week.

[0035] 3. Association analysis between survival rate traits and ZmNF...

Embodiment 2

[0039] Example 2 Amplification and sequencing of target DNA

[0040] (1) Design of primers

[0041] The DNA sequence of SEQ ID NO: 1 on chromosome 7 was downloaded from the maizeGDB website (https: / / www.maizegdb.org / gbrowse).

[0042] The DNA sequences of the designed primers are as follows:

[0043] Forward primer F: 5'-CCATGCACCATCTTTTAGGC-3' (SEQ ID NO: 2) Reverse primer R: 5'-GTGTCGGAACGGAACCACT-3' (SEQ ID NO: 3)

[0044] (2) PCR amplification

[0045] Add 1uL of DNA template, 3uL of double-distilled water, 5uL of 2X Taq PCR mix, 0.5uL of forward primer F and 0.5uL of reverse primer R to the 10ul reaction system. PCR reaction conditions were pre-denaturation at 95°C for 5 min, denaturation at 95°C for 30 s, annealing at 60°C for 30 s, 34 cycles, and a final extension at 72°C for 10 min.

[0046] (3) DNA sequence determination

[0047] The PCR products were sent to Huada Bioengineering Co., Ltd. for forward and reverse sequencing, and the following base sequences were ...

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Abstract

The invention discloses a ZmNF-YA8 gene SNP marker related to corn salt tolerance and application thereof. The SNP site is located at 5183bp downstream of the 3'UTR of the ZmNF‑YA8 gene, and there are two allelic variations: CC and TT genotypes; compared with CC genotypes, maize inbred lines containing TT genotypes are resistant to The salinity is significantly enhanced. By selecting favorable allelic variation of the marker locus to select maize materials with high salt tolerance, the breeding years of salt-tolerant maize materials can be shortened.

Description

technical field [0001] The invention belongs to the technical field of biological breeding, and in particular relates to a ZmNF-YA8 gene SNP marker related to maize salt tolerance and its application. Background technique [0002] The trend of soil salinization continues to expand around the world, which severely limits the growth of crops and has become an important factor restricting agricultural production. The increasing degree of salinization of cultivated land has seriously affected the yield and quality of maize. In order to improve the biological yield of maize in salinized land, it is necessary to carry out salt-tolerant breeding of maize. [0003] Research and production practice show that salt tolerance in maize is a complex quantitative trait, and there are significant genotypic differences in salt tolerance among different maize inbred lines. The mechanism of response to salt stress in maize is complex, and the mining of key genes with high utility value in bre...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156C12Q2600/13
Inventor 徐妙云王磊朱明靳礼安邢利娟
Owner THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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