5 results about "Colorectal tissue" patented technology

The present application relates to a kind of anaphalis contorta exosome extraction method and its application.The exosome is obtained by ultra-high speed centrifugation method from fresh anaphalis contorta, particle size is 160-200 nm, Zeta potential is-21.63±1.34 mV, with double membrane structure, mainly containing protein (such as resveratrol O-methyltransferase etc.) and phospholipid, polysaccharide content is only 12.5% of anaphalis contorta fresh medicine, and does not contain traditional active ingredient anaphalis contorta glycoside.Experiments show that anaphalis contorta exosome can be targeted to enrich in colorectal tissue, significantly improve AOM / DSS induced colorectal cancer model mouse tumor microenvironment, reduce tumor number, regulate T cell and macrophage ratio, and relieve pathological damage, better than anaphalis contorta fresh medicine.The mechanism is related to protein component, not polysaccharide or small molecule component.The anaphalis contorta exosome provided in the present application has simple preparation process, high safety, and has significant anti-colorectal cancer application potential.

This invention discloses a method for culturing organoids from normal colorectal tissue and colorectal cancer, comprising: taking tissue samples from normal colorectal tissue and colorectal cancer, and washing them; cleaning the tissue samples, cutting them into small pieces, and placing them in a buffer solution; removing the supernatant after the tissue samples have settled; adding a dissociation reagent, using a homogenizer to dissociate the tissue, and centrifuging to remove the supernatant; adding a washing buffer, refluxing to release crypts, and obtaining a tissue fragment suspension; filtering to obtain a crypt suspension, centrifuging to remove the supernatant; resuspending the precipitate with a washing buffer to obtain a culture suspension; mixing the matrix gel with the culture suspension to obtain a mixture, and counting crypts; seeding the mixture onto a multi-well plate, incubating, adding organoid culture medium, and culturing to obtain the organoids. This method improves crypt integrity and yield by determining the size of tissue fragments and using a homogenizer in the tissue dissociation process, resulting in a high success rate of organoid culture and good passage stability.

The invention discloses a nano-drug system for targeted therapy of colorectal cancer with fusobacterium nucleatum infiltration and a preparation method of the nano-drug system. The invention belongs to the technical field of biology, and particularly relates to a nano-drug system for targeted therapy of colorectal cancer with fusobacterium nucleatum infiltration and a preparation method of the nano-drug system. An active ingredient of the medicine with the tumor cell activity inhibiting effect or the anti-tumor activity contains NM-coated PLGA-MTI-OXA, the NM-coated PLGA-MTI-OXA is composed of neutrophile granulocyte membrane vesicles and PLAG-MTI-OXA, the PLAG-MTI-OXA is nanoparticles obtained by loading chemotherapeutic drugs MTI and OXA on polylactic acid-glycolic acid copolymer, and the neutrophile granulocyte membrane vesicles are derived from bone marrow neutrophile granulocytes. The NM-coated PLGA-MTI-OXA developed by the invention can effectively target colorectal tissues infiltrated by fusobacterium nucleatum, successfully realizes antibacterial and anti-tumor effects, and has application value.

The invention relates to the technical field of biomedical engineering, and discloses EO-CRC-sourced NCIO and a culture method and application thereof.The method comprises the steps that normal colorectal tissue of a premature colorectal cancer patient with the onset age smaller than 50 years old is obtained; digesting the tissue blocks for 20-60 minutes at 2-6 DEG C by using EDTA (Ethylene Diamine Tetraacetic Acid) digestive juice; stopping the digestion reaction, filtering, and centrifugally collecting a digestion product; mixing the collected digestion product with a basic culture medium and matrigel in proportion, and performing three-dimensional embedding; and after gelling, adding a normal intestinal organ-like culture medium for culturing. According to the method, culture conditions are optimized according to biological characteristics of normal tissues of a patient suffering from premature colorectal cancer, and cell damage is avoided by adopting EDTA low-temperature digestion. The cultured organoid can be stably passed for more than 15 generations, and has important application value in the fields of premature colorectal cancer disease model construction, pathogenesis research, drug screening and the like.

The utility model discloses a colorectum fixing and pulling-out auxiliary device which comprises two sets of limiting and fixing plates, the limiting and fixing plates are in an arc shape, a semicircular notch is formed in one side of each limiting and fixing plate, the two sets of limiting and fixing plates are spliced to limit the colorectum through the notches, containing grooves are formed in the two ends of each limiting and fixing plate, and the limiting and fixing plates are arranged in the containing grooves. Dirty liquid generated by an operation can slide into the containing grooves in the two ends through the surface of the arc-shaped limiting fixing plate to be collected, the dirty liquid is discharged through the drain outlets in the bottoms of the containing grooves, and limiting mechanisms are arranged at the two ends of the limiting fixing plate. According to the colorectum fixing and pulling-out auxiliary device, the two sets of limiting and fixing plates are matched with the notches to limit and fix the colorectum, medical staff can carry out resection operation on the colorectum of the limiting part, dirty liquid generated in the resection process can slide towards the two ends along the surfaces of the limiting and fixing plates and is collected in the containing grooves, and therefore the medical staff can carry out resection operation on the colorectum. And infection caused by backflow of dirty liquid into the abdominal cavity can be effectively avoided.