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Method for detecting expression quantity of tumor-related gene spectrum and application of method

A tumor-related gene and gene expression technology, applied in the field of biological and medical testing, can solve the problems of strong subjectivity and time-consuming pathological diagnosis, and achieve the effect of strong subjectivity and time-consuming

Pending Publication Date: 2022-06-28
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

Pathological morphology is the gold standard for the diagnosis of colorectal cancer. However, in recent years, there is a shortage of pathologists and pathological diagnosis takes a long time and is highly subjective; and pathological changes often lag behind those in molecular biology

Method used

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  • Method for detecting expression quantity of tumor-related gene spectrum and application of method
  • Method for detecting expression quantity of tumor-related gene spectrum and application of method
  • Method for detecting expression quantity of tumor-related gene spectrum and application of method

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Embodiment 1

[0043] In the present invention, primary colorectal cancer is the object of implementation.

[0044] Take RNA Sequencing technology to analyze the differences in mRNA expression between colorectal cancer and normal colorectal tissues; and the relationship between these differences and the identification, classification and prognosis of colorectal cancer as an example. The histological classifications of colon cancer (Colonadenocarcinoma, COAD) and colon cancer (Rectum adenocarcinoma, READ) both include Adenocarcinoma and Mucinous adenocarcinoma. Based on the study of The Cancer Genome Atlas (TCGA) database, the differences in the expression levels of a large number of colorectal cancer-related oncogenes / tumor suppressor genes between normal colorectal tissues and colorectal cancer, the relationship between expression profiles and colorectal cancer tissue types, The relationship between gene expression and prognosis of colon cancer, screen out a group of genes closely related t...

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Abstract

The invention belongs to the technical field of biological and medical examination, and relates to a molecular biological method for detecting the expression quantity of tumor-related gene products, and the method can be used for providing references for identification of colorectal cancer change, colorectal cancer classification / type, prognosis evaluation and the like. The related genes comprise: KRT23, KRT80, CA9, FOXQ1, CDH3, KIAA1199, CLDN2, KLK10, CLDN1, GRIN2D, NKD1, C6ORF223, MMP3, MMP11, TRIM29, TESC, GYLTL1B, NKD2, PRSS22, VWA2, REG1A, DPEP1, FABP6, CEL, CELP, CPNE7, RPL13AP20, TNFRSF6B, CYP19A1, RPLP0P2, GUCA2B, AQP8, CA1, GUCA2A, CA2, CLCA4, H19, FAP, NAT1 and the like. The combined application of the genes (single or any combination) is beneficial to providing references for identification, classification / type, prognosis evaluation and the like of the colorectal cancer; when the genes are jointly applied to identify colorectal cancer and normal colorectal tissues, the sensitivity can reach 97% when the specificity is 100%.

Description

technical field [0001] The invention belongs to the technical field of biological and medical testing, and relates to a method for detecting the expression level of a tumor-related gene spectrum and an application thereof. It specifically relates to a molecular biological method for detecting the expression (protein, RNA) of tumor-related gene profiles (single or in any combination), through the detection of changes in related gene profiles to provide information on the identification of colorectal cancer, colorectal cancer classification / type, and prognosis References for evaluation, etc. The genes involved include: KRT23, KRT80, CA9, FOXQ1, CDH3, KIAA1199, CLDN2, KLK10, CLDN1, GRIN2D, NKD1, C6ORF223, MMP3, MMP11, TRIM29, TESC, GYLTL1B, NKD2, PRSS22, VWA2, REG1A, DPEP1, FABP6, CEL, CELP, CPNE7, RPL13AP20, TNFRSF6B, CYP19A1, RPLP0P2, GUCA2B, AQP8, CA1, GUCA2A, CA2, CLCA4, H19, FAP, NAT1, etc. Background technique [0002] Clinical practice shows that colorectal cancer is a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/112C12Q2600/118C12Q2600/158C12Q2600/136
Inventor 刘春芳马展刘杰吕元
Owner AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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