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Schiff method for dyeing intestinal mucous cell of yellow-feather quail

A technology of mucus cells and quail, which is applied in the biological field, can solve the problems that the technology is not suitable for coloring and coloring effects

Inactive Publication Date: 2017-08-04
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The existing technology is not suitable for coloring or the coloring effect is poor

Method used

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Embodiment Construction

[0021] In order to make the objects and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0022] The embodiment of the present invention provides a Schiff's method for dyeing intestinal mucus cells of yellow-feathered quail, comprising the following steps:

[0023] S1. Weigh 0.5g of basic fuchsin and add it to 100ml of boiling distilled water, shake slightly, continue to cook for 3min, and fully dissolve. Then, when cooling to 50°C, filter with filter paper, add 10ml of 1mol / L HCl to the filtrate, and when cooling to 25°C, add 0.5g of sodium metabisulfite (Na 2 S 2 o 5 ), after fully oscillating, stopper the bottle tightly, and let it stand in a dark place at room temperature for at least 24 hours, until the red color of the solut...

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Abstract

The invention discloses a Schiff method for dyeing an intestinal mucous cell of a yellow-feather quail. The method comprises the steps of completing Schiff dye liquor preparation, selecting 1cm tissue samples of middle segments of the duodenum, the jejunum, the ileum, the cecum and the colorectum of the quail, immobilizing the tissue samples into a Bouin liquid and slicing conventional paraffin for washing; dyeing a Schiff dye liquor for 40min and washing for 10min; redyeing by using hematoxylin for 1min and carrying out differentiation and blueing; and finally carrying out washing, dehydration, transparency and mounting, wherein the dyeing result is that a goblet cell is red. The Schiff method is easy to color and stable in coloring effect.

Description

technical field [0001] The invention relates to the biological field, in particular to a Schiff's method for dyeing intestinal mucus cells of yellow-feathered quail. Background technique [0002] In the digestive tract of animals, more than 20 kinds of gastrointestinal hormone peptides have been discovered and identified. Its basic structure is a straight-chain polypeptide composed of amino acids, and its biological function depends on the type, quantity and sequence of the amino acids contained in it. There are different effects, but the more identical amino acids in the same sequence position, the closer the function is. [0003] Yellow-feathered quail is a recessive yellow-feather type, and the yellow features of the young and adult yellow-feathered quail are clearly distinguishable. The skin is white, and the beak, shanks, toes, etc. are light brown, which gradually fades with age; the adult crane quail is similar in size to the Chinese white-feathered adult quail, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30
CPCG01N1/30G01N2001/302
Inventor 何敏赵飘
Owner SICHUAN AGRI UNIV
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