5 results about "Mucous cell" patented technology

The application provides an antigen epitope peptide and a paralichthys olivaceus mucin Muc13 specific antibody prepared by the antigen epitope peptide, determines a B cell epitope from the paralichthys olivaceus Muc13 protein through antigen epitope screening, and the sequence is PGTTEEPDPTAGSPP. After the epitope is coupled with a KLH carrier protein, the epitope is used for immunizing New Zealand white rabbits, and a rabbit-derived anti-paralichthys olivaceus Muc13 specific antibody is successfully obtained. The antibody can specifically recognize the Muc13 protein in paralichthys olivaceus mucosa tissue and Muc13 positive mucous cells, and provides a key detection means for in-depth exploration of the function mechanism of mucous cells in the fish mucosa immune response process.

The application provides an antigen epitope polypeptide and a paralichthys ovatus mucin Muc5b specific antibody prepared by the antigen epitope polypeptide, a B cell antigen epitope of the paralichthys ovatus Muc5b protein is screened, the amino acid sequence of the B cell antigen epitope is shown as NDKQRSKQCEDYQVV, a New Zealand white rabbit is immunized after the antigen epitope is coupled with a KLH carrier protein, and a rabbit anti-paralichthys ovatus Muc5b specific antibody is prepared. The antibody can specifically recognize the Muc5b protein in paralichthys ovatus mucosa tissue and positive mucous cells, and provides a key detection tool for in-depth exploration of the function mechanism of mucous cells in the paralichthys ovatus mucosa immune response process.

The application provides an antigen epitope polypeptide and a paralichthys ovatus mucin Muc1 specific antibody prepared by the antigen epitope, a B cell epitope from the paralichthys ovatus Muc1 protein is identified through antigen epitope screening, and the amino acid sequence is SEQ ID NO:1. After the epitope is coupled with a KLH carrier protein, a New Zealand white rabbit is immunized, and a rabbit anti-paralichthys ovatus Muc1 polyclonal antibody is successfully prepared. The antibody can specifically recognize the Muc1 protein in paralichthys ovatus mucosa tissue and positive mucous cells, and provides an important detection tool for studying the function mechanism of mucous cells in fish mucosal immunity.

The invention discloses an intervention system for Chinese yam tuber cells based on different soil environments, and particularly relates to the technical field of Chinese yam planting intervention. A structural organic fiber material and an organic precursor material are selected to prepare a physical support carrier; a metabolic active matrix rich in extracellular enzyme systems and biostimulants is obtained; constructing a directional induction intervention layer in the underground tuber expansion area, and arranging a buffer layer and a transition structure for maintaining waterpower continuity above the directional induction intervention layer; new tubers are guided to intrude into the intervention layer, and a direct physical contact interface between plant tissues and matrix hyphae is established; in a tuber expansion period, inducing signal accumulation under micro-stress by utilizing pulse type oscillation regulation of matrix water potential, and directionally pumping precursor substances and signal molecules into cortical cells by utilizing plant transpiration pulling force in a rehydration period; the problems that in Chinese yam planting, due to the fact that microcosmic induction signals and transmission power are lacked, mucus cell differentiation is blocked, and the internal quality is low are effectively solved.

The invention provides a construction method and application of a bifluorescence reporter gene animal model for tracking transdifferentiation from airway cilia cells to mucous cells. A transgenic animal model capable of specifically and permanently marking and tracking airway ciliary cell fate transformation is constructed by utilizing a Cre-LoxP gene recombinase system and combining with double promoters and double fluorescence reporter genes. In the progeny, normal cilia cells only express GFP (green). When the cell starts to express MUC5AC under external stimulation (such as PM2.5 and allergen), the existing Cre recombinase in the cell nucleus can recognize and cut off the loxP-Stop-loxP sequence at the downstream of the MUC5AC gene, so that the expression of the RFP gene is started. Finally, mucus-cilia cells obtained by transdifferentiation of cilia cells can simultaneously express GFP (green fluorescent protein) and RFP (yellow), or only express RFP (red) after completely losing cilia cell characteristics. The irreversible color conversion provides a definite evidence for the'provenance and today 'of the cells.