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Application of chemokine CXCL14 in predicting prognosis of colon cancer

A technology for colon cancer and colorectal cancer, which is applied in the preparation of test samples, measurement devices, and color/spectral characteristics measurement, etc. levels, improving treatment outcomes, and improving quality of life

Pending Publication Date: 2019-11-22
成都和同易创生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the surgical operation, the judgment of tumor tissue is an extremely critical factor. Sometimes it is impossible to judge whether the tumor has invaded healthy tissue with the naked eye, so the resection is not clean and causes recurrence, and sometimes blindly enlarged resection leads to resection. Unnecessary organs, causing a decrease in the patient's quality of life
Currently commonly used tumor markers for colorectal cancer include CEA, CA19-9, CA72-4, and CA242, but the false positive rate of these markers in serum is still high, which is still not as good as the gold standard pathological section come exactly

Method used

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  • Application of chemokine CXCL14 in predicting prognosis of colon cancer
  • Application of chemokine CXCL14 in predicting prognosis of colon cancer
  • Application of chemokine CXCL14 in predicting prognosis of colon cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] A method for using a diagnostic kit for detecting the chemokine CXCL14 for predicting the prognosis of colorectal cancer, comprising the following steps:

[0047] (1) The clinical tissue was embedded in paraffin and then serially sectioned, with a thickness of 2 μm;

[0048](2) Place the cut slices in a constant temperature oven at 55°C for 2h;

[0049] (3) dewaxing is carried out to the above-mentioned dried section;

[0050] (4) soaking the dewaxed tissue sections with PBS solution;

[0051] (5) Add 50ul of endogenous peroxidase blocking agent to each section and incubate at room temperature for 20 minutes;

[0052] (6) Rinse or soak in PBS for 3×3min;

[0053] (7) Add 50ul of non-immune animal serum to each tissue section to block non-specific binding;

[0054] (8) Dry the serum, add the diluted primary antibody rabbit-derived anti-CXCL14 polyclonal antibody to each slice, and keep it at 4°C overnight;

[0055] (9) Rinse or soak in PBS for 3×3min;

[0056] (10)...

Embodiment 2

[0064] The preparation of tissue microarray (TMA) includes the following steps:

[0065] (1) Make a mold wax block with a size of 45mm × 20mm, punch holes with a spacing of 0.1mm and a diameter of 0.6mm on the mold wax block, and each hole is accurately positioned with coordinates.

[0066] (2) According to the observation of HE staining, the sample paraffin block was selected and marked on the HE section and the corresponding paraffin tissue block, respectively, and the site of the sampling tissue was punctured with a stainless steel needle with an inner diameter of 0.6 mm, with a depth of 2 to 3 mm, and it was fixed on the Inside the hole in the mold wax block.

[0067] (3) The mold wax block was sliced ​​with a thickness of 4 μm, and it was applied to a solid phase carrier such as a glass slide or nylon membrane to make a tissue microarray, and then immunohistochemical staining could be performed.

Embodiment 3

[0069] The correlation between the expression of chemokine CXCL14 protein and the metastasis and survival rate of colorectal cancer

[0070] 1. Tissue Microarray

[0071] The preparation of tissue microarray was carried out with reference to Example 2.

[0072] Tissue microarrays (TMA) of 50 colorectal cancer tissues and 40 paracancerous tissues were prepared for immunohistochemical analysis of CXCL14 to determine the correlation of CXCL14 protein expression level with prognosis.

[0073] Another tissue microarray (TMA) of 50 primary colorectal cancer lesions and their corresponding lymph node metastases was prepared for immunohistochemical analysis of CXCL14 to compare the differences in CXCL14 protein expression between primary and metastatic lesions, and then determine Correlation of CXCL14 protein expression level with tumor metastasis.

[0074] 2. Immunohistochemistry

[0075] Referring to the method of Example 1, step (1) was replaced with the above-mentioned tissue m...

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Abstract

The invention discloses the application of chemokine CXCL14 in predicting the prognosis of colon cancer, and the application of a reagent for detecting the chemokine CXCL14 in the preparation of a tool for predicting the prognosis of colorectal cancer is included. The invention particularly discloses a method for quantitatively detecting the expression of CXCL14 protein in colorectal tissues through an immunological method to predict the prognosis of the colon cancer. The method has a significant predictive value for the prognosis of the total survival time of a patient with an III-IV colorectal cancer, therefore, the poor treatment prognosis can be improved in a targeted manner, the medical level is improved, and the quality of life is improved.

Description

technical field [0001] The present invention relates to the field of prediction of cancer prognosis, in particular, discloses the application of chemokine CXCL14 in predicting the prognosis of colon cancer. Background technique [0002] Colorectal cancer is a relatively common type of malignant tumor, not only has a high incidence rate, but also has a relatively high mortality rate. At present, its morbidity and mortality rates are among the highest in the world. In 2018 alone, the number of new cases reached nearly 2 million, and the number of deaths was nearly 900,000. Judging from the current clinical treatment situation, the incidence of colorectal cancer in cities is generally 2-3. The proportion of young people under 40 years old with colorectal cancer accounts for about 20% of the total number of colorectal cancers, and further rising trend. [0003] Although the level of diagnostic technology and treatment of colorectal cancer is constantly improving, its postopera...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N1/36G01N1/30G01N21/31
CPCG01N33/57446G01N1/36G01N1/30G01N21/31G01N2001/302G01N2800/065
Inventor 黄灿华黄琛张涛
Owner 成都和同易创生物科技有限公司
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