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Application of stem cell exosome in preparation of drug for resisting colitis deterioration

A technology of exosomes and stem cells, applied in the field of medicine and biology, to achieve the effect of good application prospect, convenient source and low immunogenicity

Pending Publication Date: 2020-10-13
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the methods used clinically to treat inflammatory bowel disease (IBD) include traditional drugs, immunosuppressants, biological agents, antibiotics and surgical treatment, etc., but these methods still have their limitations, and new treatment methods are urgently needed clinically To intervene, inhibit or delay the occurrence of colorectal cancer caused by malignant transformation of colorectal inflammation

Method used

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  • Application of stem cell exosome in preparation of drug for resisting colitis deterioration
  • Application of stem cell exosome in preparation of drug for resisting colitis deterioration
  • Application of stem cell exosome in preparation of drug for resisting colitis deterioration

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Preparation of exosomes from human umbilical cord mesenchymal stem cells

[0027] 1. Preparation of human umbilical cord mesenchymal stem cells

[0028] Mesenchymal stem cells are obtained from fresh umbilical cords.

[0029] The specific operation is: take the fresh umbilical cord out of the biological safety cabinet, put it into a petri dish containing PBS, and wash away the residual blood as much as possible. The umbilical cord was cut into several sections and two umbilical arteries and one umbilical vein were removed. Put the Wharton's jelly part of the umbilical cord from which the blood vessels were removed into a new cell culture dish, add PBS containing 0.1% penicillin-streptomycin until completely submerged, and wait for 30 minutes. Take another cell culture dish, add a dropper containing 15% FBS α-MEM nutrient solution, and cut the Wharton's jelly part of the umbilical cord into 0.1cm in a humid environment 2 Tissue blocks of left and right size ...

Embodiment 2

[0038] Example 2 Using the Exosomes Prepared in Example 1 to Intervene the Mouse Malignant Transformation Model

[0039] Such as Figure 4 , using AOM / DSS combined to induce the malignant transformation of IBD in mice, and intervening with exosomes derived from human umbilical cord mesenchymal stem cells via tail vein injection. The experiment was divided into normal control group (Normal group), AOM / DSS-induced IBD malignant transformation model group (AOM / DSS group), hucMSC-Ex intervention group (hucMSC-Ex group, that is, exocytosis derived from human umbilical cord mesenchymal stem cells). Body intervention group): feeding 6-week-old, male BALB / C mice.

[0040] Except for the Normal group, the other two groups were intraperitoneally injected with 1 mg / ml AOM solution on the 0th day, and the dose was 10 mg / kg. On the 7th day, the 28th day and the 49th day, each mouse started to drink 2.5% DSS water prepared by autoclaving pure water for 1 week, and each mouse in the hucMSC...

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Abstract

The invention provides application of a stem cell exosome in preparation of a drug for resisting colitis deterioration. A mouse colorectal cancer (CRC) model is induced in an AOM / DSS combined induction manner, the mouse weight, disease activity index (DAI), colorectal tissue pathologic structure and colorectal tissue deterioration degree are detected, and results show that the exosome derived fromhuman umbilical cord mesenchymal stem cells (hucMSCs) can inhibit the colorectal deterioration process. The invention belongs to biotherapy, and the exosome delays or inhibits the colitis deterioration process of a model animal by inhibiting expression of SUMO1 protein, has the characteristics of low immunogenicity, source convenience and the like and has good application prospects in resisting the colitis deterioration process.

Description

technical field [0001] The invention relates to the field of medical biotechnology, in particular to the application of stem cell exosomes in the preparation of anti-colitis exacerbation drugs. Background technique [0002] Colorectal cancer (CRC) is a malignant tumor of the digestive tract with a high incidence in humans and the third most common malignant tumor in the world. Repeated damage, repair and proliferation of chronic inflammation can easily cause gene mutation and cell canceration. Studies have shown that about 25% of cancers are derived from chronic inflammation. For example, inflammatory bowel disease (IBD) is an important pathogenic factor of colorectal cancer, a large Increased risk of colorectal cancer. Thus, effective treatment of colonic inflammation is of great significance for the prevention of colorectal cancer. [0003] Currently there is no therapeutic approach against malignant transformation of colitis, and the main focus is on monitoring and prev...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61P35/00A61P1/00A61P29/00
CPCA61K35/28A61P1/00A61P29/00A61P35/00
Inventor 毛飞王静燕王茹冰严永敏张徐钱晖许文荣
Owner JIANGSU UNIV
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