LncRNA biomarkers for diagnosing human lung adenocarcinoma and human colorectal cancer

A biomarker, colorectal cancer technology, applied in the field of LncRNA biomarkers for the diagnosis of human lung adenocarcinoma and human colorectal cancer

Inactive Publication Date: 2014-11-05
SHANGHAI INST OF MICROSYSTEM & INFORMATION TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the biological function of LncRNA in lung adenocarcinoma and colorectal cancer and the relationship between the expression of LncRNA and lung adenocarcinoma and colorectal cancer are still unknown.

Method used

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  • LncRNA biomarkers for diagnosing human lung adenocarcinoma and human colorectal cancer
  • LncRNA biomarkers for diagnosing human lung adenocarcinoma and human colorectal cancer
  • LncRNA biomarkers for diagnosing human lung adenocarcinoma and human colorectal cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1 Clinical sample collection:

[0041] A total of 113 pairs of frozen fresh tissue specimens of early lung adenocarcinoma and their paired normal lung tissues were collected from Zhongshan Hospital Affiliated to Fudan University during 2011-2014. All patients gave informed consent to participate in the scientific research.

[0042] 2 LncRNA quantitative PCR experiment:

[0043] 2.1 RNA extraction and quantitative PCR

[0044] Total RNA was extracted from frozen tissues using Trizol, and RNA was quantified using a microvolume spectrophotometer.

[0045] 2.2 Internal reference genes and primers

[0046] Internal reference gene selection GAPDH, GAPDH and a set of primer sequences of LncRNA biomarkers provided by the present invention are shown in Table 1:

[0047] Table 1 Primer sequences used in quantitative PCR

[0048]

[0049]

[0050] 2.3 Reverse transcription reaction

[0051] Use the SuperScriptⅢFirst Strand Synthesis System kit for reverse transcription...

Embodiment 2

[0076] 1 Clinical sample collection:

[0077] 18 pairs of fresh frozen tissue specimens of colorectal cancer and their paired normal colorectal tissues were collected from Ruijin Hospital Affiliated to Shanghai Jiaotong University in 2013. All patients gave informed consent to participate in the scientific research.

[0078] 2 LncRNA quantitative PCR experiment:

[0079] 2.1 RNA extraction and quantitative PCR

[0080] Total RNA was extracted from frozen tissues using Trizol, and RNA was quantified using a microvolume spectrophotometer.

[0081] 2.2 Internal reference genes and primers

[0082] GAPDH was selected as the internal reference gene, and the primer sequences of the internal reference gene and each LncRNA biomarker are shown in Table 1 in Example 1.

[0083] 2.3 Reverse transcription reaction, real-time quantitative PCR reaction

[0084] The specific methods, steps and reaction system of the reverse transcription reaction and the real-time quantitative PCR reactio...

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Abstract

The invention relates to the technical field of biology, and in particular relates to a group of LncRNA biomarkers for diagnosing human lung adenocarcinoma and human colorectal cancer, related kits and application thereof. The group of LncRNA biomarkers for diagnosing human lung adenocarcinoma and / or human colorectal cancer comprises uc001gzl.3, ENST00000434223, uc004bbl.1, ENST00000540136 and NR_034174. The LncRNA biomarkers for diagnosing the human lung adenocarcinoma and / or human colorectal cancer can be used for distinguishing human early lung adenocarcinoma and human colorectal cancer, the AUC of the biomarkers for distinguishing early lung adenocarcinoma from normally paired lung tissues can reach 0.978, and the sensitivity and the specificity are respectively 92% and 98%. The AUC of the biomarkers for diagnosing a colorectal cancer sample can reach 0.963, and the sensitivity and the specificity are respectively 94.4% and 88.9%.

Description

technical field [0001] The present invention relates to the field of biotechnology, in particular to a group of LncRNA biomarkers for diagnosis of human lung adenocarcinoma and human colorectal cancer, related kits and their uses. Background technique [0002] Non-small cell lung cancer is the leading cause of cancer-related death worldwide. It mainly includes four histological subtypes: adenocarcinoma, squamous cell carcinoma, large cell carcinoma, and others (neuroendocrine carcinoma, benign tumor, etc.). The five-year survival rate of non-small cell lung cancer is less than 15%, and early diagnosis can effectively reduce its mortality. Early detection of lung adenocarcinoma is especially important because it is the most common type of non-small cell lung cancer. Understanding the genetic and molecular dysregulation mechanisms of lung adenocarcinoma is the key to early diagnosis of lung adenocarcinoma. [0003] Colorectal cancer is one of the common malignant tumors of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6886C12Q2600/178
Inventor 毛红菊王萍卢韶华白亚楠张宏莲金庆辉赵建龙
Owner SHANGHAI INST OF MICROSYSTEM & INFORMATION TECH CHINESE ACAD OF SCI
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