The invention belongs to the field of
biotechnology and microbial animal
cell lines and particularly relates to a method for cultivating
lymph-node autologous CIK (
cytokine-induced killer) cells and application of the
lymph-node autologous CIK cells. The CIK cells are obtained by in-vitro cultivation of isolated
lymph node tissue obtained in
surgery in patients with
lung cancer and are compared with the CIK cells obtained from cultivation of isolated
peripheral blood, biological characteristics of the CIK cells and
cytotoxicity effect on pulmonary granule
cancer cells are observed, the result shows that the isolated
lymph node issue of the
surgical patients can be completely used for cultivation of CIK, in-vitro tumor-
cytotoxicity cells are powerful in activity, and the activity and
cancer inhibition function are prior to the CIK cells of the
peripheral blood. The CIK cells can be used for preparing pulmonary granule and cancer inhibition products and made into compounds with
chemotherapy drugs as well as used for
adoptive immunity and intervention for postoperative patients with
lung cancers, and occurrence rate for recurrence and
metastasis of the postoperative patients with the
lung cancers is beneficially decreased.