The invention belongs to the field of 
biotechnology and microbial animal 
cell lines and particularly relates to a method for cultivating 
lymph-node autologous CIK (
cytokine-induced killer) cells and application of the 
lymph-node autologous CIK cells. The CIK cells are obtained by in-vitro cultivation of isolated 
lymph node tissue obtained in 
surgery in patients with 
lung cancer and are compared with the CIK cells obtained from cultivation of isolated 
peripheral blood, biological characteristics of the CIK cells and 
cytotoxicity effect on pulmonary granule 
cancer cells are observed, the result shows that the isolated 
lymph node issue of the 
surgical patients can be completely used for cultivation of CIK, in-vitro tumor-
cytotoxicity cells are powerful in activity, and the activity and 
cancer inhibition function are prior to the CIK cells of the 
peripheral blood. The CIK cells can be used for preparing pulmonary granule and cancer inhibition products and made into compounds with 
chemotherapy drugs as well as used for 
adoptive immunity and intervention for postoperative patients with 
lung cancers, and occurrence rate for recurrence and 
metastasis of the postoperative patients with the 
lung cancers is beneficially decreased.