ANXA3 gene or ANXA3 protein as biomarker of lung adenocarcinoma

A lung adenocarcinoma and genetic technology, applied in the determination/testing of microorganisms, DNA/RNA fragments, biological testing, etc., can solve the problem that the five-year survival rate of patients is less than 15%

Inactive Publication Date: 2019-01-18
FIRST AFFILIATED HOSPITAL OF KUNMING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the treatment of molecular targeted drugs has achieved gratifying results in recent years, there are still 70% of Xuanwei lung adenocarcinoma patients who are already in the advanced stage of cancer when they are diagnosed with lung cancer and have distant metastasis. Five of these patients The annual survival rate is less than 15%, which brings huge economic burden to society and family

Method used

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  • ANXA3 gene or ANXA3 protein as biomarker of lung adenocarcinoma
  • ANXA3 gene or ANXA3 protein as biomarker of lung adenocarcinoma
  • ANXA3 gene or ANXA3 protein as biomarker of lung adenocarcinoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Expression of ANXA3 gene in lung adenocarcinoma

[0037] 1 Gene chip detection of gene expression

[0038] 1.1 Materials

[0039] Surgically resected cancer tissues and paracancerous tissues of 29 patients with primary lung adenocarcinoma were used as experimental samples. All patients with lung adenocarcinoma lived in Xuanwei area for more than ten years, and all cancer tissues were pathologically confirmed as lung adenocarcinoma. All patients with primary lung adenocarcinoma had not received radiotherapy and chemotherapy before operation, and the clinical data of all cases were complete.

[0040] 1.2 Extraction of tissue RNA

[0041]The RNA in each tissue was extracted by TRIZOL method, and the concentration and purity of the extracted RNA were determined by Nanodrop2000, and the OD260 / 280 was required to be between 1.8 and 2.2. Store at -80°C for later use.

[0042] 1.3 Fluorescence labeling of samples

[0043] a. Synthesis of First strand cDNA by reverse trans...

Embodiment 2

[0063] Expression of ANXA3 Protein in Lung Adenocarcinoma

[0064] 1Clinical specimen collection and lung adenocarcinoma tissue information

[0065] The collection of specimens and the preparation of case data are the same as in the first part. In this part, 21 pairs of primary lung adenocarcinoma tissue and paracancerous specimens were detected.

[0066] 2 Tissue protein extraction and concentration measurement

[0067] The RIPA lysis method was used to extract the total protein in the tissue, and the BCA protein concentration assay kit was used to determine the protein concentration, using the Biyuntian kit, catalog number: P0012S.

[0068] 3 protein denaturation

[0069] Each protein sample was adjusted according to the measured sample concentration, and an appropriate amount of 5×loadingbuffer was added to each tube, boiled in a metal bath for 5 minutes, and stored at -20°C for each use.

[0070] 4Western Blot experimental steps

[0071] Prepare SDS-PAGE gel according ...

Embodiment 3

[0076] Immunohistochemical expression of ANXA3 protein in lung adenocarcinoma

[0077] 1. Research materials

[0078] A total of 69 specimens of primary lung adenocarcinoma in the Xuanwei area were collected from the First Affiliated Hospital of Kunming Medical University from 2016 to 2017. A total of 72 cases of cancer specimens. A total of 141 cases of lung adenocarcinoma were collected. Before surgery, the patients had no history of radiotherapy and chemotherapy. The collection of all cases included the tumor part and the paracancerous area, and relatively complete clinical data were obtained. In addition, human spleen tissue was selected as the positive control photo of the ANXA3 antibody.

[0079] Among the 69 cases of primary lung adenocarcinoma in Xuanwei area, there were 43 males and 26 females, including 39 smokers and 30 non-smokers. The age ranges from 32 to 77 years old, the average age and median age are 60 and 52 years old respectively. There were 23 cases wi...

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Abstract

The invention relates to the technical field of lung cancer gene detection, in particular to an ANXA3 gene or an ANXA3 protein as a biomarker of lung adenocarcinoma. Research findings, ANXA3 gene wasabnormally expressed in lung adenocarcinoma in both Xuanwei and non-Xuanwei areas. The expression of ANXA3 gene was lower or higher than normal level. ANXA3 gene or protein could be used as biomarkerof lung adenocarcinoma to diagnose lung adenocarcinoma, judge lymph node metastasis, assist TNM staging and prognosis. The invention provides an ANXA3 gene and an expression product thereof in preparation for prejudging whether lymph node metastasis of lung adenocarcinoma, The present invention provides a new diagnostic method for clinically diagnosing metastasis of lung adenocarcinoma and provides a new candidate drug for treating metastasis of lung adenocarcinoma. The present invention relates to a method for treating metastasis of lung adenocarcinoma and a pharmaceutical composition for treating lung adenocarcinoma.

Description

technical field [0001] The invention relates to the technical field of lung cancer diagnosis, in particular to ANXA3 gene or ANXA3 protein as a lung adenocarcinoma biomarker. Background technique [0002] Annexin A3, one of the 12 members of the annexin membrane protein family, is a calcium-dependent phospholipid-binding protein. The expression of ANXA3 protein is specific, and ANXA3 protein has multiple functions, involved in anti-inflammation, anti-fibrinolysis, vesicle transport, cell proliferation, differentiation, apoptosis, cell growth and angiogenesis. Recent research results show that ANXA3 plays an important role in liver regeneration and angiogenesis in rats, the expression of ANXA3 is abnormal in lung cancer and prostate cancer, and the expression level of ANXA3 is closely related to tumor prognosis. [0003] The incidence of lung cancer ranks first among all kinds of solid tumors in the world, and ranks first in cancer-related deaths, especially primary lung ade...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886G01N33/68G01N33/574C12N15/11
CPCC12Q1/6886G01N33/57407G01N33/57423G01N33/68G01N2333/4718
Inventor 赵滢张艳亮马丽菊苏艳丹段勇
Owner FIRST AFFILIATED HOSPITAL OF KUNMING MEDICAL UNIV
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