MiRNA marker in serum exosome related to early diagnosis of lung adenocarcinoma and application

A technology of exosomes and markers, applied in the fields of biotechnology and diagnostics, to achieve the effect of convenient diagnosis and improved clinical treatment effect

Inactive Publication Date: 2017-05-31
FIRST AFFILIATED HOSPITAL OF DALIAN MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these serological markers are broad-spectrum cancer diagnostic molecular markers, and their role in the specific diagnosis and early diagnosis of lung cancer has great limitations.

Method used

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  • MiRNA marker in serum exosome related to early diagnosis of lung adenocarcinoma and application
  • MiRNA marker in serum exosome related to early diagnosis of lung adenocarcinoma and application
  • MiRNA marker in serum exosome related to early diagnosis of lung adenocarcinoma and application

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Embodiment 1

[0026] Serum exosome extraction:

[0027] 5ml of cubital venous blood was collected with a coagulation-promoting tube, centrifuged at 3000rpm for 10min, and the supernatant serum was absorbed. The serum was centrifuged at 4°C, 20000g for 30min, and the cell debris was discarded. The obtained serum crude extract was filtered through a 0.22um filter membrane, added with PBS, and centrifuged at 4°C and 150000g for 90min. After centrifugation, discard the PBS, add PBS to suspend and wash the precipitate, and centrifuge at 4°C and 150,000g for 60min. Serum exosomes were obtained, and 500ul Trizol was added to extract RNA.

[0028] Sample total RNA extraction:

[0029](1) Before the experiment, wipe the experimental bench with absolute ethanol to reduce the dust on the experimental bench and disinfect it; (2) Take out the sample and thaw it; (3) After centrifuging the lysate containing Trizol LS at 12000rmp for 10min at 4°C, absorb the supernatant To an RNase-free centrifuge tub...

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Abstract

The invention discloses a miRNA marker in a serum exosome related to early diagnosis of lung adenocarcinoma and application. The marker is selected from hsa-miR-4516, hsa-miR-342-3p, hsa-miR-199a-3p, hsa-miR-150-5p, hsa-miR-140-3p, hsa-miR-18a-5p, hsa-miR-222-3p, hsa-miR-27a-3p, hsa-miR-29a-3p, hsa-miR-221-3p, hsa-miR-500a-3p, hsa-miR-6126 and hsa-miR-6715a-3p. The marker can effectively separate early lung adenocarcinoma cases from healthy people. The marker and probes thereof can be used for preparing a lung adenocarcinoma early diagnosis kit. The marker has an assisting effect on early diagnosis of lung adenocarcinoma.

Description

technical field [0001] The invention belongs to the field of biotechnology and diagnostics, in particular to a serum exosome miRNA marker for early diagnosis of lung adenocarcinoma and its application. Background technique [0002] Lung cancer diagnosis lacks early diagnostic methods and specific biomarkers [0003] Lung cancer is the second most common cancer worldwide (prostate cancer with the highest incidence in men; breast cancer with the highest incidence in women). More than 1.2 million people die from lung cancer every year in the world, accounting for about 28% of the global death toll. Although some molecular targeted therapies currently used in the diagnosis and treatment of lung cancer are quite effective, the five-year survival rate of patients is still not optimistic. The reason is that there is a lack of early diagnostic methods and diagnostic molecular markers, and most lung cancer patients are already at an advanced stage when they are diagnosed. [0004]...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6886C12Q2600/158C12Q2600/178
Inventor 刘晶孙瑜王亮冯敏李琳马静云
Owner FIRST AFFILIATED HOSPITAL OF DALIAN MEDICAL UNIV
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