Serum exosome hsa-miR-17-5p related to diagnosis and treatment of cancers and application thereof

Abstract

The invention discloses a serum exosome hsa-miR-17-5p related to the diagnosis and treatment of cancers and application thereof. The invention first protects the application of a substance for assaying the hsa-miR-17-5p in the preparation of a product; and the function of the products is (c1) or (c2) as follows: (c1) identifying or helping to identify liver cancer cells; (c2) diagnosing or helpingto diagnose liver cancer. The invention further protects a primer group, which is prepared from a reverse transcription primer, a sense primer and an antisense primer; the reverse transcription primer is a single-chain DNA molecule shown as sequence 2 in a sequence table; the sense primer is a single-chain DNA molecule shown as sequence 3 in the sequence table; and the antisense primer is a single-chain DNA molecule shown as sequence 4 in the sequence table. The serum exosome hsa-miR-17-5p can serve as a biomarker for liver cancer, and has high diagnostic value, a rapid and accurate diagnostic method is provided for a clinic, and the diagnosis of liver cancer becomes more convenient and easier to implement.

Description

technical field [0001] The invention belongs to the field of biotechnology and diagnosis, and specifically relates to serum exosome hsa-miR-17-5p related to the diagnosis and treatment of liver cancer and its application. Background technique [0002] Liver cancer is a malignant tumor with the fourth highest incidence rate and the second highest mortality rate in the world. Because of the relationship between diet, alcoholic liver disease, especially liver cirrhosis, and hepatitis B, liver cancer has a high incidence and high risk in China. Many liver cancers are usually found in clinically advanced stages, making the 5-year survival rate of liver cancer in my country less than 10%. Therefore, the early diagnosis of liver cirrhosis and liver cancer is very important. At present, the main blood marker used for the diagnosis of liver cancer is alpha-fetoprotein (AFP), but its accuracy is not good, and its sensitivity and specificity are not enough to meet the early detection...

AI Technical Summary

Problems solved by technology

At present, the main blood marker used for the diagnosis of liver cancer is alpha-fetoprotein (AFP), but its accuracy is not good, and its sensitivity and specificity are not enough to meet the early detection of liver cancer

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