Fructus terminaliae billericae extract with anti-cancer effect, and preparation method of effective part of fructus terminaliae billericae extract

The technology of extracts and parts is applied in the field of preparation of anticancer extracts of Myrobalan japonica and effective parts thereof, and can solve the problems of low content of active components in extraction process, incomplete extraction and enrichment process, unclear research on anticancer activity and the like, Achieve the effect of reducing production cost and toxicity, low price and simplifying process flow

Active Publication Date: 2019-03-12
BEIJING UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention relates to the preparation method of the anticancer extract of Myrobalan and its active part, including the extraction of the part containing the active ingredient, and the process research of enrichment by using a macroporous adsorption resin, and also includes the identification of the active ingredient in the active ingredient, It solves the long-term lack of clear anticancer activity research, incomplete extraction and enrichment process, and low content of active ingredients in the existing extraction process.

Method used

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  • Fructus terminaliae billericae extract with anti-cancer effect, and preparation method of effective part of fructus terminaliae billericae extract
  • Fructus terminaliae billericae extract with anti-cancer effect, and preparation method of effective part of fructus terminaliae billericae extract
  • Fructus terminaliae billericae extract with anti-cancer effect, and preparation method of effective part of fructus terminaliae billericae extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Soak the HPD826 resin material in 95% ethanol solvent for 24 hours, elute dynamically with 5BV95% ethanol solvent, and then wash with water until odorless; soak in 5% HCl solution for 3 hours, elute with 3BV, and wash with water until medium Nature; after soaking in 5% NaOH solution for 3 hours, 3BV elutes, wash with water until neutral; then wash with 95% ethanol solvent until the eluent is mixed with water (1:5) without white turbidity; then wash with water until odorless ,spare;

[0030] (2) Take the medicinal material of myrobalan, crush it through a 5-mesh sieve, use 10 times the amount of 50% aqueous ethanol solvent to extract 3 times, each time for 10 hours, filter the extract, and store it at -0.06~-0.09MPa, 45~50℃ , recovered under reduced pressure, concentrated to obtain an extract with anticancer active ingredients, the content of active ingredients in the extract with anticancer active ingredients is 19.76%;

[0031] (3) Dilute the above-mentioned extra...

Embodiment 2

[0034] (1) Soak the HPD400 resin material in 90% ethanol solvent for 12 hours, then dynamically elute with 10BV90% ethanol solvent, and then wash with water until odorless; soak in 5% HCl solution for 3 hours, elute with 3BV, and wash with water until medium Nature; after soaking in 5% KOH solution for 3 hours, 3BV elutes, wash with water until neutral; then wash with 90% ethanol solvent until the eluent is mixed with water (1:5) without white turbidity; then wash with water until odorless ,spare;

[0035] (2) Take the medicinal material of myrobalan, crush it through a 10-mesh sieve, use 8 times the amount of 70% aqueous ethanol solvent to extract once, each time for 8 hours, filter the extract, and store it at -0.06~-0.09MPa, 45~50℃ , recovered under reduced pressure, concentrated to obtain an extract with anticancer active ingredients, the content of active ingredients in the extract with anticancer active ingredients is 19.26%;

[0036] (3) Dilute the above-mentioned extr...

Embodiment 3

[0039] (1) Soak the AB-8 resin material in 100% ethanol solvent for 48 hours, elute dynamically with 4BV100% ethanol solvent, and then wash with water until odorless; soak in 5% sulfuric acid solution for 3 hours, elute with 3BV, and wash with water to neutral; after soaking in 5% NaOH solution for 3 hours, 3BV was eluted, washed with water until neutral; then washed with 100% ethanol solvent until the eluent was mixed with water (1:5) without white turbidity; then washed with water until tasteless, spare;

[0040] (2) Take the medicinal material of myrobalan, crush it through a 7-mesh sieve, use 12 times the amount of 50% aqueous ethanol solvent to extract twice, each time for 12 hours, filter the extract, and store it at -0.06~-0.09MPa, 45~50℃ , recovered under reduced pressure, and concentrated to obtain an extract with anticancer active ingredients, and the content of the active ingredients in the extract with anticancer active ingredients is 19.31%;

[0041] (3) Dilute t...

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Abstract

The invention relates an anticancer active part of fructus terminaliae billericae, and a preparation method of the anticancer active part. The invention comprises extraction of the part containing active components, study of a process for enriching by adopting macroporous absorption resin, identification of the active components in the active part, and use of the active part in in-vivo or in-vitroinhibition of liver cancer HepG2, lung cancer A549, lung adenocarcinoma NCI-H1703, gastric cancer BGC823, osteosarcoma cell MG-63, colorectal cancer HCT116, breast cancer MCF-7, neuroblastoma cells shsy5y, kidney cancer ACHN, normal liver cells L02, human breast ductal carcinoma cells ZR75-1, human colorectal adenocarcinoma cells Colo-205, human breast ductal carcinoma cells BT-474, human breastcancer cells T-47D, human cervical cancer cell line HeLa, liver cancer cells H22, and the like. The enrichment method applying the macroporous absorption resin to the anti-cancer active part of the fructus terminaliae billericae is simple in process, safe, non-toxic and low in production cost, and can be used for industrial production, thus having a great economic benefit and higher generalizationperformance.

Description

technical field [0001] The present invention relates to the anticancer extract of chebula and the preparation method of its effective part, and also relates to the pharmaceutical use of the active part. Background technique [0002] Myrobalan was first recorded in "Xinxiu Materia Medica", which is a plant of the Gentleman family Pilele Terminalia billerica ( Gaertn. ) dry ripe fruit of Roxb. Also known as Junbaonai, Galazhuma, Zuobeixiang, Mohe, Ruka, Cimusi, Baana, Nahe, Manjid, Duli, Palula, etc., it is a commonly used Tibetan medicine and Mongolian medicine. And dimension medicine. The fruit of Myrobalan is bitter, astringent, and flat in nature. It has the effects of clearing away heat and detoxification, astringent and nourishing blood, and harmonizing various medicines. It is used to treat various fevers, dysentery, yellow water disease, liver and gallbladder disease, and weakness after illness. Saprogenizes muscle, heals wounds, and makes skin moist and shiny. I...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P35/00A61K131/00
CPCA61P35/00A61K36/185A61K2236/333A61K2236/39A61K2236/53A61K2236/51A61K2236/55
Inventor 张兰珍梁文仪陈文静梁林金叶婷
Owner BEIJING UNIV OF CHINESE MEDICINE
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