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Composition related to lung adenocarcinoma metastasis and application thereof

A technology of lung adenocarcinoma and composition, which is applied in the composition and its application fields related to the metastasis of lung adenocarcinoma, and can solve the problems affecting the expression of target genes

Inactive Publication Date: 2015-07-22
BEIJING MEDINTELL BIOMED CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, miRNAs sometimes lead to histidine modification and DNA methylation in the promoter region, thereby affecting the expression of target genes.

Method used

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  • Composition related to lung adenocarcinoma metastasis and application thereof
  • Composition related to lung adenocarcinoma metastasis and application thereof
  • Composition related to lung adenocarcinoma metastasis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] The collection of embodiment 1 sample

[0066] The tumor tissues of 10 cases of lung adenocarcinoma were all obtained from surgically resected specimens of Beijing Friendship Hospital from January 2009 to December 2009. All specimens were placed in a liquid nitrogen tank within 10 minutes of isolation, and then transferred to a -80°C refrigerator Storage, after 3 years of follow-up, no metastasis occurred in 3 patients, and metastasis occurred in 7 patients.

Embodiment 2

[0067] Example 2 total RNA extraction

[0068] 1 extraction method

[0069] 1) Take 80 mg tissue block, add 800 μl Lysis / Binding buffer, and use a homogenizer to homogenize the tissue block. During the homogenization process, the samples should be placed on ice to keep the cold state.

[0070] 2) Add 1 / 10 volume of Homogenate Additive to the above homogenized tissue samples, and place on ice for 10 minutes.

[0071] 3) Add an equal volume of water-saturated phenol to the Lysis / Binding buffer, shake for 45 seconds, and centrifuge at 10,000×g for 5 minutes at room temperature.

[0072]4) Carefully remove the supernatant into a new test tube, add 1.25 times the volume of absolute ethanol, mix well, transfer to a purification column, centrifuge at 10,000×g for 15 s, and discard the liquid in the collection tube. Since the maximum volume of the column is only 700 μl, repeat this step until all the supernatant is filtered.

[0073] 5) Add 700 μl of miRNA eluent 1 to the spin col...

Embodiment 3

[0082] Example 3 Sequencing and Data Analysis

[0083] The sequencing company carried out the establishment of the sequencing library and on-machine sequencing, and the sequencer used was the HiSeq2000 sequencer of Illumina Company.

[0084] According to the data analysis results provided by the company: the metastatic group (7 cases) and the non-metastatic group (3 cases) were statistically analyzed, the P value was less than 0.05, and the difference between the metastatic group and the non-metastatic group was at least more than 2 times of differential expression miRNA, mir-193a, mir-3942, mir-767, mir-193b, mir-550a-1 with significantly up-regulated expression in the artificial selection and filtering of differentially expressed miRNAs entered our research scope.

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Abstract

The invention relates to a composition related to lung adenocarcinoma metastasis and application thereof and particularly relates to novel application of the composition composed of one or more of the mir-193a, mir-3942, mir-767, mir-193b and mir-550a-1 and / or matures miRNA for diagnosing the lung adenocarcinoma metastasis. By performing sequencing analysis on the lung adenocarcinoma metastatic group and the non-metastatic group, a reserved drone gene is chosen to perform molecular biology verification. The result shows that the miRNA provided by the invention is closely related to the lung adenocarcinoma metastasis. The composition can be used for clinical diagnosis and preventive detection and has good practical application value.

Description

technical field [0001] The present invention relates to the field of molecular biology, in particular to a composition related to metastasis of lung adenocarcinoma and its application, more specifically to the composition of mir-193a, mir-3942, mir-767, mir-193b, mir-550a-1 A new application of one or several and / or a composition composed of mature miRNA in the diagnosis and treatment of metastasis of lung adenocarcinoma. Background technique [0002] Lung adenocarcinoma (lung adenocarcinoma) belongs to non-small cell lung cancer, which is prone to occur in women and non-smokers. Tumors expand more slowly (doubling time about 120 days) in the lungs than in the periphery. There are no symptoms in the early stage, and it is usually diagnosed at an advanced stage. Non-small cell lung cancer accounts for 75%-80% of the total number of lung cancers. In my country, the death due to lung cancer accounts for about 23% of all tumor-related deaths, and about 90% of malignant tumor ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61K31/7088A61P35/00C12Q1/68
Inventor 杨承刚董东宋宏涛
Owner BEIJING MEDINTELL BIOMED CO LTD
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