28 results about "Dextran sulfate" patented technology

The invention discloses a preserving fluid for stably preserving sample DNA at normal temperature and a preparation method thereof, and belongs to the technical field of biological sample preservation. The preserving fluid comprises a lysis system, a nucleic acid protection system and a buffering and stabilizing system, the cracking system comprises a composite surfactant and an enzymolysis auxiliary agent; the composite surface active agent is prepared from polyether polyol fatty acid ester and cocamidopropyl hydroxy sulfobetaine; the enzymolysis auxiliary agent comprises lysozyme Lyso-V and protease K; the nucleic acid protection system comprises a nitrogen heterocyclic polyamine-carboxylic acid derivative and dextran sulfate; the nitrogen heterocyclic polyamine-carboxylic acid derivative comprises 1, 4, 7, 10-tetraazacyclododecane-N, N ', N' ', N ''tetraacetic acid, 1, 4, 7-triazacyclononane-N, N', N''-triacetic acid, disodium ethylene diamine tetraacetate-nitrogen heterocyclic derivative, and diethylenetriamine pentaacetic acid-piperazine derivative, and the nitrogen heterocyclic polyamine-carboxylic acid derivative comprises 1, 4, 7, 10-tetraazacyclododecane-N, N ', N '', N'' tetraacetic acid, 1, 4, 7-triazacyclononane-N, N', N ''-triacetic acid. The buffering and stabilizing system comprises an amphoteric buffering agent and a polymer stabilizer; the amphoteric buffering agent comprises 2-(N-morpholino) ethanesulfonic acid and N-tri (hydroxymethyl) methylglycine.

This invention relates to an absorbable, slow-release regulator for off-season antler growth in sika deer and red deer, and its preparation method, belonging to the field of off-season antler growth regulators. The regulator comprises 330 mg–360 mg medroxyprogesterone acetate (MPA), 50 mg–55 mg dextran sulfate (DS), and 200 mg–220 mg sodium hyaluronate (HA), diluted with water for injection to a final volume of 1.0 mL. The molecular weight of medroxyprogesterone acetate (MPA) is 386.52 kDa; the molecular weight of dextran sulfate (DS) is 36–50 kDa; and the molecular weight of sodium hyaluronate (HA) is 20–400 kDa. This invention utilizes exogenous progesterone to induce off-season antler growth in male deer through hormonal regulation. The use of absorbable materials is environmentally friendly and harmless to the body, allowing for natural absorption and normal excretion through the body's metabolic processes. A single injection is sufficient to provide efficacy for over 35 days. Using progesterone to regulate off-season antler growth is low-cost and has a high success rate.

This invention belongs to the field of vertebrate technology, specifically relating to a method for constructing a complex inflammatory animal model in middle-aged and elderly individuals and its related applications. The method includes: selecting adult animals for adaptive feeding, daily subcutaneous injection of D-galactose to establish a susceptibility to aging and oxidative stress, and administering sodium dextran sulfate (DSS) solution within a specific time window to induce intestinal inflammation, forming a complex inflammatory state through gut-hepatic axis interactions. The constructed animal model simultaneously exhibits intestinal inflammatory damage, abnormal hepatic oxidative stress, and aging-related characteristics, realistically simulating the pathological process of gut-hepatic axis dysfunction in middle-aged and elderly individuals. This model is suitable for screening and evaluating drugs, medical nutrition products, and functional foods that improve gut-hepatic axis dysfunction, providing a reliable technical platform for research on related disease mechanisms and the development of interventional substances.

The invention discloses a drug delivery system containing loaded adriamycin as well as a preparation method and application of the drug delivery system. The drug delivery system comprises poly (beta-amino ester) coupled with adriamycin, wherein the surface of the poly (beta-amino ester) is coated with dextran sulfate, and the poly (beta-amino ester) takes a fourth-generation lysine dendritic molecule Lys-G4 as a core. The drug delivery system has a pH-dependent drug release characteristic, has remarkable toxicity to C6 and U87MG glioma cells, has the capabilities of inducing apoptosis, inhibiting migration and targeting subcellular localization, and can effectively penetrate through a blood-brain barrier model and 3D tumor spheres. The delivery system is compounded with PLA-PEG-PLA thermosensitive hydrogel, and the thermosensitive hydrogel with a drug storage function is successfully constructed. Good tumor inhibition effect and biocompatibility are shown in nude mouse subcutaneous and in-situ glioma models. The invention not only provides a new strategy for overcoming the blood brain barrier, but also can effectively reduce the systemic toxicity, and opens up a new research thought and method for glioma treatment.

The invention belongs to the technical field of biology, and particularly relates to a bacteriostatic agent and a preparation method and application thereof. Comprising the following steps that antibacterial peptide modified silver nanoparticles are prepared, specifically, antibacterial peptide and silver nitrate grow in situ under the action of a reducing agent, and the antibacterial peptide modified silver nanoparticles are obtained; preparing a bacteriostatic agent: dissolving the antibacterial peptide modified silver nanoparticles by using a cross-linked substance of chitosan and dextran sulfate to obtain the bacteriostatic agent; the charge of the cross-linked substance of the chitosan and the dextran sulfate is 0. Through mutual attraction of positive charges of the antibacterial peptide and negative charges of the silver nanoparticles, the silver nanoparticles wrapped by the antibacterial peptide are formed, the self-assembly or irregular agglomeration behavior of the antibacterial peptide is effectively controlled, a nanometer network formed by crosslinking chitosan with dextran sulfate can stabilize the nanometer form of the silver nanoparticles, and the antibacterial activity of the antibacterial peptide is improved. The core-shell structure formed by the silver nanoparticles wrapped by the antibacterial peptide can be stabilized, the silver nanoparticles wrapped by the antibacterial peptide can also be dispersed, and mutual agglomeration of the core-shell structure is prevented.

This invention provides a soil conditioner prepared from Phyllanthus emblica pomace and its preparation method, relating to the field of plant by-product processing technology. The soil conditioner comprises Phyllanthus emblica pomace, animal manure, biochar, modified starch, modified pectin, sodium dextran sulfate, a compound microbial agent, and Sphingomonas sphingosine monocytogenes. The Phyllanthus emblica pomace is first soaked in sodium chloride solution, then fermented and filtered with Sphingomonas sphingosine monocytogenes. The filtered residue is then sterilized, mixed with animal manure, and composted. The filtrate is mixed with other substances and then combined with the composted fertilizer. This invention overcomes the shortcomings of existing technologies, effectively regulates alkaline soil, and to a certain extent, can remediate heavy metals in the soil and promote crop growth.

This invention discloses a preservation solution for preserving sample DNA at room temperature and its preparation method, belonging to the field of biological sample preservation technology. The preservation solution comprises: a lysis system, a nucleic acid protection system, and a buffering and stabilizing system; the lysis system comprises a complex surfactant and an enzymatic hydrolysis aid; the complex surfactant comprises: polyether polyol fatty acid ester and cocamidopropyl hydroxysulfonate betaine; the enzymatic hydrolysis aid comprises lysozyme Lyso-V and proteinase K; the nucleic acid protection system comprises: nitrogen-heterocyclic polyamine-carboxylic acid derivatives and dextran sulfate; the nitrogen-heterocyclic polyamine-carboxylic acid derivatives comprise: 1,4,7,10-tetraazacyclododecane-N,N',N'',N'''tetraacetic acid, 1,4,7-triazacyclononane-N,N',N''-triacetic acid, disodium ethylenediaminetetraacetate-nitrocyclic derivative, and diethylenetriaminepentaacetic acid-piperazine derivative; the buffering and stabilizing system comprises: an amphoteric buffer and a polymeric stabilizer; the amphoteric buffer comprises: 2-(N-morpholino)ethanesulfonic acid and N-tris(hydroxymethyl)methylglycine.

The application relates to the technical field of pharmaceutical preparations, in particular to a florfenicol preparation with high water solubility and bacteriostatic effect and a preparation method thereof.The raw materials of the florfenicol preparation with high water solubility and bacteriostatic effect include, by mass fraction, florfenicol 20-30 parts, beta-cyclodextrin 30-50 parts, solid polyethylene glycol 30-50 parts, sodium dodecyl sulfate 2-5 parts, dopamine aqueous solution 100-200 parts and dextran sulfate aqueous solution 1-5 parts; wherein the concentration of the dopamine aqueous solution is 1-3 g / L, and the concentration of the dextran sulfate aqueous solution is 0.1-0.5 mol / L.The application has a wide application range, is commonly used for granulation of heat-sensitive materials and drugs which are easily decomposed in water, and is convenient for subsequent re-compression tabletting or encapsulation; and compared with a wet granulation method, the application has the advantages of simple process, low energy consumption, small filler consumption and the like.

This invention, entitled "A Composition Containing Pentadecanoic Acid, Parabacterium Species Outer Membrane Vesicles Containing the Composition, and Their Applications," belongs to the field of probiotics and microbial preparations technology. The technical problem this invention aims to solve is that beneficial live bacteria have weak colonization ability in the gut, are easily affected by the gastrointestinal environment, exhibit unstable individual responses, and struggle to simultaneously address immune regulation and barrier repair. This invention provides a composition containing pentadecanoic acid, Parabacterium Species-derived outer membrane vesicles, and their applications. The outer membrane vesicles are derived from *Parabacterium difficile* and are rich in pentadecanoic acid, palmitic acid, and octadecanoic acid. The composition and outer membrane vesicles prepared by this invention can significantly inhibit macrophage M1 polarization and reduce pro-inflammatory cytokines. TNF-α , IL-6 and IL-1 β It can lower the expression level of [something], reduce intracellular reactive oxygen species levels, improve mitochondrial function, significantly alleviate symptoms of colitis induced by sodium dextran sulfate, regulate the intestinal flora structure, and increase the abundance of beneficial bacteria.

The invention provides application of barley pest larva defatted protein powder in preparation of drugs for treating inflammatory bowel diseases. The invention belongs to the technical field of medicine, and by establishing a dextran sodium sulfate induced mouse IBD model and lavaging barley pest larva defatted protein powder, it is found that the traditional Chinese medicine composition can relieve IBD mouse colitis symptoms, restore colon length, relieve mucosal lesion, retain goblet cell and crypt structures, reduce inflammatory cell infiltration and down-regulate inflammatory cytokine expression; the compound is expected to play an important role in preparing medicines for treating inflammatory enteritis.

The invention relates to a preservation method and a preservation solution of exosomes, and belongs to the technical field of biology, the preservation solution comprises trehalose, cane sugar, sodium hyaluronate, polyethylene glycol, dextran sulfate, an HEPES buffer solution and mannitol, the preservation method comprises the following steps: mixing purified exosomes with the exosome protection solution to obtain a mixture, and adding the mixture into the exosome protection solution to obtain the exosome preservation solution. Sub-packaging the mixture, and directly cryopreserving the sub-packaged sample at the temperature of-80 DEG C or lower; in a word, through a rigid network formed by the sodium hyaluronate and the polyethylene glycol, the aggregation and fusion of the exosome in the freezing and thawing process are effectively inhibited. The embodiment shows that after three times of freeze-thaw cycles, the particle size change rate of the exosome is less than 6%, and the particle recovery rate is close to 90%.

The present application relates to the technical field of veterinary medicine, and discloses a kind of absorbable sustained-release agent for inhibiting the fighting behavior of male deer during estrus, comprising the following steps: stirring micronized medroxyprogesterone acetate with dextran sulfate and water for injection to obtain a homogeneous suspension paste; adding sodium hyaluronate dry powder without adding solvent to knead, and through competitive water absorption, dextran sulfate is dehydrated to form a physical coating layer on the surface of drug particles, obtaining a non-saturated hard rubber mass; after cooling, the remaining water for injection is pre-cooled and forced to pass through a microporous screen to achieve reciprocating convection extrusion, and then the product is obtained by swelling after standing.The present application utilizes the double retardation effect of interface physical coating layer and three-dimensional network of gel to inhibit the initial drug burst of long-acting preparation, prevent the gravity settlement of drug particles, achieve the long-term stable release of medroxyprogesterone acetate in animal body, and further reduce the fighting behavior of male deer during estrus.

This invention provides the application of *Pleurotus oosporum* polysaccharide in the preparation of drugs and health foods that regulate intestinal barrier function or protect against intestinal damage-related diseases. Its protective effect against a mouse model of intestinal damage induced by sodium dextran sulfate was investigated. Results showed that *Pleurotus oosporum* polysaccharide significantly improved symptoms such as weight loss, loose stools, and colonic atrophy caused by intestinal damage in mice, reduced the disease activity index, inhibited colonic epithelial destruction, inflammatory cell infiltration, and goblet cell necrosis, and enhanced intestinal mucosal barrier function. As a fungus, *Pleurotus oosporum* has high safety and is a potential source of active ingredients against intestinal damage, showing promising development prospects in intestinal barrier protection.

This invention relates to suspended cells, specifically to an anti-suspension cell clumping complex and its preparation method, and its application. The anti-suspension cell clumping complex is characterized by comprising sodium dextran sulfate, sodium heparin, and non-essential amino acids. The complex of this invention can reduce the cell clumping rate after 4 days of large-scale suspended cell culture (7.18%-10.27%). The optimal formulation shows a clumping rate as low as 7.18% (≤10%), significantly superior to sodium dextran sulfate (DSS), sodium heparin, non-essential amino acid groups, and commercially available anti-clumping agents. It effectively solves the problem of cell aggregation and clumping in large-scale, high-density culture of cells adapted to suspension.

This invention relates to a rapid film-forming repair composition, its preparation method, and its application. The rapid film-forming repair composition comprises: a rapid film-forming repair component, a film softener, a pH adjuster, a preservative, and water. The film-forming repair component includes: Suizenji nori polysaccharide, galactoarabinogalactan, and sodium dextran sulfate. The three components, Suizenji nori polysaccharide, galactoarabinogalactan, and sodium dextran sulfate, interact synergistically to promote skin improvement. Specifically, they can accelerate the formation of a film after application of the active ingredients, effectively conditioning and repairing the skin. Furthermore, they can alleviate skin inflammation and redness, and promote tissue regeneration. The rapid film-forming repair composition provided by this invention is highly suitable for use in skincare products, being gentle, non-irritating, and stable.

The invention discloses a double mRNA (messenger Ribonucleic Acid) combined targeted delivery system for treating a lower limb peripheral artery disease (PAD) and application of the double mRNA combined targeted delivery system. The system comprises a microcrystal nanocarbon targeted drug-loading delivery matrix on which messenger ribonucleic acid (mRNA) of an IL-4 gene and / or a VEGF gene is loaded. The matrix comprises matrix microcrystalline nanocarbon, and folic acid (FA), dextran sulfate (DS), polyethyleneimine (PEI) and polyethylene glycol (PEG) which are modified on the surface of the matrix microcrystalline nanocarbon. According to the system, folic acid and dextran sulfate are used for dual targeting of macrophages, so that efficient endocytosis is realized; the mRNA is used for rapidly expressing protein in cytoplasm, so that the risk of genome integration is avoided; through co-delivery of IL-4 and VEGF, functional angiogenesis and tissue repair are synergistically promoted. Preferably, the system can be entrapped in injectable hydrogel to form a gel composite system, so that slow and continuous release of the medicine is realized. The invention further provides a preparation method of the system and application of the system in preparation of drugs for treating ischemic diseases. The system has the advantages of high targeting efficiency, high expression speed, high vascular maturity, lasting effect and the like, and provides a new strategy for PAD treatment.

This invention relates to the field of animal reproductive regulation technology, and discloses a twinning regulator for sika deer males that synchronize estrus and its application method. The regulator is a homogeneous and transparent sol system prepared from trehalose, pituitary follicle-stimulating hormone (FSH), medium- and low molecular weight sodium hyaluronate, low molecular weight dextran sulfate, zinc gluconate, and physiological saline. This invention utilizes sodium hyaluronate of a specific molecular weight and dextran sulfate to construct a polysaccharide sol network, combined with the cross-linking effect of zinc gluconate and the physicochemical stabilizing effect of trehalose, to encapsulate and slowly release FSH. The application method includes implanting and removing a vaginal silicone plug, combined with the injection of pregnant mare serum gonadotropin (PMSG) and the regulator, followed by natural mating. This invention prolongs the half-life of exogenous hormones in the animal, avoids drastic fluctuations in blood drug concentration, and improves the stability of twinning rates in sika deer.

The invention provides a cassiterite collecting agent and a flotation method of sulfur-containing micro-fine particle cassiterite fine silt. The cassiterite collecting agent is formed by mixing benzohydroxamic acid and sodium dextran sulfate. Or the cassiterite collecting agent is a carboxymethyl chitosan-catechol polymer. The combination of a cassiterite collecting agent carboxymethyl chitosan-catechol polymer or benzohydroxamic acid and dextran sulfate sodium has a unique dual-action mechanism of'strong anchoring-bridging flocculation '. Benzohydroxamic acid and dextran sulfate sodium or catechol groups form super-strong coordination with Sn < 4 + > on the cassiterite surface, so that strong anchoring is realized; and a plurality of micro-fine particle cassiterite can be simultaneously adsorbed by a macromolecular long chain to form an easily floated aggregate, so that bridging flocculation is realized. By means of the cassiterite collecting agent, the capturing capacity of 20-micron ultra-fine cassiterite can be remarkably improved. Compared with conventional benzohydroxamic acid, the recovery rate of the micro-fine particle cassiterite is increased by 8-15%, and maximization of resource utilization is achieved.

The present invention aims to provide a composition useful for inhibiting angiogenesis or vascular loss. ru. [Solution] In one embodiment of the present invention, (A) dextran sulfate or a salt thereof, (B) Prepare a composition containing royal jelly extract and (C) bilberry leaf extract. In one embodiment, in the manufacture of an external composition, dextran sulfate or its salt, rh The invention also relates to the use of compositions containing jelly extract and bilberry leaf extract.

The invention belongs to the technical field of molecular biological detection, and discloses a reaction system, a kit and a detection method applied to RNA amplification of a complex whole blood sample. The reaction system comprises a buffer component and an enzyme protection component; the buffer component consists of Tris and Bicine and is used for cooperatively maintaining the stability of the pH value of a reaction system in the RNA amplification process of a complex whole blood sample; and the enzyme protection component is composed of AEBSF and dextran sulfate. Through collaborative innovation of a double-buffer system and an active enzyme protective agent, the integrated challenges that the pH is easy to drift, the template compatibility is poor and the enzyme activity is easy to damage when a complex whole blood sample is faced in the prior art are effectively overcome, and a key technical support is provided for rapid, stable and reliable bedside molecular diagnosis.

The invention relates to the technical field of biochemical detection, and discloses a dry chemical test paper for quantitative determination of human whole blood high density lipoprotein cholesterol content, which comprises a diffusion layer, a blood filter layer, a pre-precipitation layer, a precipitation layer, a supplementary precipitation layer and a reaction layer which are sequentially stacked from top to bottom, the pre-precipitation layer is coated with a first reagent, the first reagent contains a precipitant I, the precipitation layer is coated with a second reagent, the second reagent contains a precipitant II, the supplementary precipitation layer is coated with a third reagent, and the third reagent contains a precipitant III; the precipitant I, the precipitant II and the precipitant III respectively adopt at least one of dextran sulfate, polyethylene glycol 2000, phosphotungstic acid and polyethyleneimine, and the precipitant I, the precipitant II and the precipitant III adopt different formulas. The dry chemical test paper can realize simple, rapid, high-precision and high-accuracy quantitative determination of high-density lipoprotein cholesterol in human whole blood, and solves the technical problems of low detection accuracy and precision of existing dry chemical test paper.

The invention discloses a preparation method of a chromatographic purification medium for efficiently separating subunit viruses. Comprising the following steps: (1) preparing a base frame material; (2) adding dextran sulfate sodium salt into the base frame material; (3) adding a phosphate buffer solution, a phase transfer catalyst and a cross-linking agent; (4) reacting under a staged alkaline pH condition, wherein the pH of the second stage is higher than that of the first stage; and (5) after the reaction is finished, washing with alkali liquor and purified water for multiple times to obtain the chromatography purification medium. The molecular weight of the dextran sulfate sodium salt is 25 to 35 kDa, preferably 30 kDa. The base frame material is agarose microspheres, the agarose microspheres are prepared from agarose with the concentration of 3.0%-3.5%, the particle size of the agarose microspheres is 45-165 microns, the mass ratio of the agarose microspheres to the purified water to the dextran sulfate sodium salt is 5: (1-1.5), the phosphate buffer solution is tripotassium phosphate or sodium phosphate, and the concentration of the phosphate buffer solution is 0.005-0.02 mol / L, preferably 0.01 mol / L.

The invention discloses a preparation method of a ferritin blank serum matrix, which comprises the following steps: defibrating: adjusting the pH value of plasma to 7.5 + / -0.2, adding a calcium chloride solution, stirring and centrifuging to obtain defibrated supernate; degreasing: adding a calcium chloride solution and a dextran sulfate sodium salt solution into the defibrated supernate, stirring, standing, centrifuging, removing sediment, adding a barium chloride solution, stirring, and centrifuging to obtain degreased supernate; immunoadsorption: mixing immunomagnetic beads coated with a mouse anti-human ferritin antibody with the degreased supernatant to carry out immunoadsorption reaction, and carrying out magnetic separation to obtain an adsorbed supernatant; and dialysis: dialyzing the supernatant after adsorption to obtain the ferritin blank serum matrix. According to the method, the clean blank matrix liquid can be obtained on the basis of reserving plasma protein as much as possible.

The application belongs to the technical field of bioactive substance separation, and discloses a magnetic dextran sulfate composite carrier, a preparation method thereof and a collagen extraction method. The preparation of the magnetic dextran sulfate composite carrier comprises the following steps: mixing Fe3O4 nanoparticles with water and performing ultrasonic dispersion treatment to obtain a mixed dispersion system; mixing the mixed dispersion system with dextran sulfate and stirring, and then performing magnetic field separation, water washing and vacuum drying to obtain the magnetic dextran sulfate composite carrier. The magnetic dextran sulfate composite carrier is adopted to realize efficient separation and high-purity extraction of collagen, the carrier can be recycled, the production cost is reduced, and the application is suitable for industrial production.

The application provides an adsorbent for treating cerebral apoplexy, a preparation method thereof and a perfusion device. The adsorbent comprises a carrier and a first functional layer and a second functional layer arranged on the surface of the carrier in sequence; the carrier has epoxy groups; the first functional layer is a grafted layer formed by long-chain linear aliphatic diamines reacting with the epoxy groups on the carrier, one amine group in the long-chain linear aliphatic diamines is connected with the epoxy groups, and the other amine group in the long-chain linear aliphatic diamines is located on the surface of the first functional layer; and the second functional layer is a grafted layer formed by dextran sulfate connecting with the amine groups on the surface of the first functional layer. The application can effectively adsorb blood lipids, fibrinogen and inflammatory factors in blood.

According to the present invention, an in situ hybridization method is provided that is more sensitive and has fewer false positives than conventional methods. [Solution] A method for detecting a gene abnormality in which the structure of a chromosome is abnormal, comprising the steps of: labeling at least one nucleic acid of 10kb to 300kb; performing situ hybridization using the labeled nucleic acid in a buffer containing dextran sulfate with a molecular weight of 3,000 to 70,000 daltons; and detecting the hybridized nucleic acid.

The invention discloses a qPCR (quantitative polymerase chain reaction) freeze-drying reagent for triple detection of influenza A, influenza B and novel coronavirus as well as a preparation method and application of the qPCR freeze-drying reagent. The reagent is prepared by freeze-drying a liquid premixed solution containing an enzyme, a primer probe, dNTPs, Mg, a freeze-drying protective agent and an additive. According to the invention, a formula of a specific additive consisting of betaine and dextran sulfate is adopted, and a conventional freeze-drying protective agent (trehalose, polysucrose and BSA) is cooperated, so that the technical problems of easy enzyme activity inactivation, poor storage stability, non-uniform multi-reaction efficiency after redissolution and the like of a multiple qPCR reagent in the freeze-drying process are effectively solved. The reagent disclosed by the invention can be stably stored and transported at normal temperature, can realize high-sensitivity, high-specificity and high-reproducibility detection of three pathogens only by one-step sample adding operation during use, and is particularly suitable for on-site rapid detection and large-scale screening.

The invention relates to dextran sulfate, or a pharmaceutically acceptable salt thereof, in treatment of sepsis and hypercytokinemia.