Determination reagent of total bile acid

Abstract

The invention relates to a determination reagent of a total bile acid. The reagent comprises two components, wherein the reagent 1 comprises a phosphate buffer, Thio-NAD (Nicotinamide Adenine Dinucleotide), Emulgen B-66 CTAB (Cetyltrimethyl Ammonium Bromide), NaCl, 2-hydroxypyridine-N-oxide; the reagent 2 comprises a glycinate buffer, dextran sulfate, EGTA (Ethylene Glycol Tetraacetic Acid), 3 alpha-HSD (Hydrogen Sterol Dehydrogenase), Emulgen B-66, NADH (Nicotinamide-adenine Dinucleotide Acid), and 2-hydroxypyridine-N-oxide. As the combination of the cation surfactant and the nonionic surfactant Emulgen B-66 is added, the interference of bilirubin can be effectively eliminated; the selected stabilizer is added in the product, so that the reagent can be stored for one year at 2-8 DEG C without influence on the properties; the substrate concentration and the ion strength can be adjusted to enable the recovery rate of the reagent to be more than 98%. The determination reagent disclosed by the invention has the advantages of high accuracy in determined results, strong anti-interference performance, good stability and the like.

Description

technical field [0001] The invention relates to an in vitro diagnostic reagent, in particular to a reagent for measuring total bile acids. Background technique [0002] Serum total bile acids can reflect the three aspects of liver cell secretion, synthesis, and damage at the same time. Experiments have shown that the positive rate of total bile acids for liver cirrhosis and liver cancer is as high as 95%. The popularity of total bile acids detection has made various acute and chronic hepatitis, The early diagnosis of liver cirrhosis and even liver cancer patients has a more effective basis, and the clinical diagnosis is of great significance. [0003] As early as the 1970s, the Norwegian Comyn company successfully produced the first generation of enzymatic bile acid reagents using 3α-hydroxysteroid dehydrogenase (3α-HSD), making the determination of bile acids truly enter the clinical field. Subsequently, the method for the determination of total bile acids developed rapidl...

AI Technical Summary

Problems solved by technology

[0004] Most of the total bile acid reagents currently used have poor stability. The validity period of the products under refrigerated conditions is mostly less than 10 months, the thermal stability at 37 degrees is less than 5 days, and the reagents are stable at two within a week (determined value continues to decline); and most brands of total bile acid reagent bilirubin negative interference is obvious, and the recovery rate of the reagent is low, most of the reagent recovery rate is about 80%, and some are even as low as 60%, so that clinical Samples often have negative values

Therefore, there are many limitations in the application of reagents from most manufacturers at present.

Images