The invention discloses a pulsed steam sterilization process suitable for sterilizing medical equipment and a steam sterilizer for the steam sterilization process. The sterilization process comprises the following steps of vacuumizing, preheating, sterilizing, performing vacuum drying and the like. The steam sterilizer comprises a steam sterilization box, a valve bank, an air filter, a steam generator, a vacuum pump and an automatic control system. Compared with the prior art, the invention has the advantages that: by the pulsed steam sterilization process and the steam sterilizer thereof, a sterilization apparatus can be transported without packaging, the temperature raising and reduction time of the sterilization is shortened, the sterilization time of the apparatus is shortened, the labor intensity of medical care personnel is reduced, and the disinfection and sterilization are more complete and have a better effect.