A kind of exosome freeze-dried powder and its preparation method and preparation comprising the exosome freeze-dried powder

A technology of exosomes and freeze-dried powder, applied in prostheses, pharmaceutical formulations, bone/connective tissue cells, etc., can solve the problems of poor filling effect, large immune rejection, short maintenance time, etc., to achieve convenient storage, promote Effects of cell differentiation and regeneration and lowering glass transition temperature

Active Publication Date: 2022-01-11
CHENGDU QINGKE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the above-mentioned deficiencies in the prior art, the present invention provides a freeze-dried exosome powder and its preparation method and a preparation containing the freeze-dried exosome powder, which can effectively solve the problem of existing cosmetic preparations. Poor filling effect, short maintenance time and large immune rejection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A kind of exosome freeze-dried powder, its preparation method comprises the following steps:

[0027] (1) Preparation of platelet-rich plasma: take autologous peripheral blood, centrifuge at 800g / min for 6min, then absorb the middle part, freeze it at -40°C for 3h, take it out and freeze-thaw it at 2000rpm / min Centrifuge for 6 minutes, remove the solid debris at the bottom, and obtain platelet factor-rich plasma, which is set aside;

[0028] (2) Preparation of exosomes: Absorb autologous fat to prepare P3-generation adipose-derived mesenchymal stem cells, culture P3-generation adipose-derived mesenchymal stem cells, and wash the cells with normal saline when the degree of cell confluence reaches 75%. Then the cells were transferred to the serum-free medium and cultured for 20 hours, the cell culture supernatant was collected, centrifuged at 2°C and 800g centrifugal force for 10min, discarded the precipitate, and then carried out ultrasonography under the condition of th...

Embodiment 2

[0033] A kind of exosome freeze-dried powder, its preparation method comprises the following steps:

[0034] (1) Preparation of platelet-rich plasma: take autologous peripheral blood, centrifuge at 2000g / min for 12min, then absorb the middle part, freeze it at -80°C for 8h, take it out and freeze-thaw it at 4000rpm / min Centrifuge for 10 minutes, remove the solid debris at the bottom, and obtain platelet factor-rich plasma, which is set aside;

[0035] (2) Preparation of exosomes: Absorb autologous fat to prepare P6-generation adipose-derived mesenchymal stem cells, culture P6-generation adipose-derived mesenchymal stem cells, and wash the cells with normal saline when the degree of cell confluence reaches 85%. Then the cells were transferred to serum-free medium and cultured for 30 hours, and the cell culture supernatant was collected, centrifuged at 6°C and 1200g centrifugal force for 20 minutes, discarded the precipitate, and then subjected to ultrasonography under the condi...

Embodiment 3

[0040] A kind of exosome freeze-dried powder, its preparation method comprises the following steps:

[0041] (1) Preparation of platelet-rich plasma: take autologous peripheral blood, centrifuge at 1500g / min for 10min, then absorb the middle part, freeze it at -80°C for 6h, take it out and freeze-thaw it at 3000rpm / min Centrifuge for 8 minutes, remove the solid debris at the bottom, and obtain platelet factor-rich plasma, which is set aside;

[0042] (2) Preparation of exosomes: Absorb autologous fat to prepare P4 adipose-derived mesenchymal stem cells, culture P4 adipose-derived mesenchymal stem cells, and wash the cells with normal saline when the degree of cell confluence reaches 80%. Then the cells were transferred to serum-free medium and cultured for 24 hours, and the cell culture supernatant was collected, centrifuged at 4°C and 1000g centrifugal force for 15 minutes, discarded the precipitate, and then subjected to ultrasonography under the condition of a current carry...

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PUM

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Abstract

The invention discloses a freeze-dried exosome powder, a preparation method thereof, and a preparation containing the freeze-dried exosome powder. The preparation method of the freeze-dried exosome powder includes the following steps: preparation of platelet-rich factor plasma: taking Autologous blood, prepared by centrifugation, prepared by exosomes; obtained from autologous fat, prepared adipose-derived mesenchymal stem cells, and cultured the mesenchymal stem cells; prepared by lyophilization: platelet-rich factor plasma, and exosomes The body is mixed, and trehalose is added therein, and mixed to obtain the preparation; freeze-drying: the freeze-dried liquid is subjected to freeze-drying to obtain the preparation. The preparation includes freeze-dried exosome powder, hyaluronic acid and physiological saline, which can effectively solve the problems of poor filling effect, short maintenance time and large immune rejection in existing cosmetic preparations.

Description

technical field [0001] The invention relates to the technical field of freeze-dried preparations, in particular to a freeze-dried exosome powder, a preparation method thereof, and a preparation containing the freeze-dried exosome powder. Background technique [0002] With the growth of age, the face will gradually appear sunken, wrinkled, skin aging, etc., which is a big blow to people who are born with beauty. How to restore their face and skin to a youthful state has also become their pursuit. Aiming at this goal, various facial filling materials and technologies were born, such as hyaluronic acid, umbilical cord blood products, etc., but most of the current filling materials have short maintenance time, poor filling effect or large immune rejection, which leads to patients The problem of poor sensitivity. [0003] Exosomes are nanoscale lipid bilayer vesicles released by exocytosis to the outside of cells, and can exist in various types of extracellular body fluids, such...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0775A61L27/38A61L27/36A61L27/20A61L27/50
CPCA61L27/3834A61L27/3616A61L27/20A61L27/50A61L2400/06C08L5/08
Inventor 高雪华海泉
Owner CHENGDU QINGKE BIOTECH
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