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Exosome freeze-drying powder, preparation method of exosome freeze-drying powder and preparation comprising exosome freeze-drying powder

A technology of exosomes and freeze-dried powder, which is applied in prosthetics, pharmaceutical formulations, medical science, etc., can solve problems such as short maintenance time, poor filling effect, and large immune rejection, and achieve lower glass transition temperature and convenient The effect of storage and prolonging the maintenance time

Active Publication Date: 2019-09-27
CHENGDU QINGKE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the above-mentioned deficiencies in the prior art, the present invention provides a freeze-dried exosome powder and its preparation method and a preparation containing the freeze-dried exosome powder, which can effectively solve the problem of existing cosmetic preparations. Poor filling effect, short maintenance time and large immune rejection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A kind of exosome freeze-dried powder, its preparation method comprises the following steps:

[0027] (1) Preparation of platelet-rich plasma: take autologous peripheral blood, centrifuge at 800g / min for 6min, then absorb the middle part, freeze it at -40°C for 3h, take it out and freeze-thaw it at 2000rpm / min Centrifuge for 6 minutes, remove the solid debris at the bottom, and obtain platelet factor-rich plasma, which is set aside;

[0028] (2) Preparation of exosomes: Absorb autologous fat to prepare P3-generation adipose-derived mesenchymal stem cells, culture P3-generation adipose-derived mesenchymal stem cells, and wash the cells with normal saline when the degree of cell confluence reaches 75%. Then the cells were transferred to the serum-free medium and cultured for 20 hours, the cell culture supernatant was collected, centrifuged at 2°C and 800g centrifugal force for 10min, discarded the precipitate, and then carried out ultrasonography under the condition of th...

Embodiment 2

[0033] A kind of exosome freeze-dried powder, its preparation method comprises the following steps:

[0034] (1) Preparation of platelet-rich plasma: take autologous peripheral blood, centrifuge at 2000g / min for 12min, then absorb the middle part, freeze it at -80°C for 8h, take it out and freeze-thaw it at 4000rpm / min Centrifuge for 10 minutes, remove the solid debris at the bottom, and obtain platelet factor-rich plasma, which is set aside;

[0035] (2) Preparation of exosomes: Absorb autologous fat to prepare P6-generation adipose-derived mesenchymal stem cells, culture P6-generation adipose-derived mesenchymal stem cells, and wash the cells with normal saline when the degree of cell confluence reaches 85%. Then the cells were transferred to serum-free medium and cultured for 30 hours, and the cell culture supernatant was collected, centrifuged at 6°C and 1200g centrifugal force for 20 minutes, discarded the precipitate, and then subjected to ultrasonography under the condi...

Embodiment 3

[0040] A kind of exosome freeze-dried powder, its preparation method comprises the following steps:

[0041] (1) Preparation of platelet-rich plasma: take autologous peripheral blood, centrifuge at 1500g / min for 10min, then absorb the middle part, freeze it at -80°C for 6h, take it out and freeze-thaw it at 3000rpm / min Centrifuge for 8 minutes, remove the solid debris at the bottom, and obtain platelet factor-rich plasma, which is set aside;

[0042] (2) Preparation of exosomes: Absorb autologous fat to prepare P4 adipose-derived mesenchymal stem cells, culture P4 adipose-derived mesenchymal stem cells, and wash the cells with normal saline when the degree of cell confluence reaches 80%. Then the cells were transferred to serum-free medium and cultured for 24 hours, and the cell culture supernatant was collected, centrifuged at 4°C and 1000g centrifugal force for 15 minutes, discarded the precipitate, and then subjected to ultrasonography under the condition of a current carry...

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Abstract

The invention discloses exosome freeze-drying powder, a preparation method of the exosome freeze-drying powder and a preparation comprising the exosome freeze-drying powder. The preparation method of the exosome freeze-drying powder comprises the following steps of: preparing platelet factor-rich plasma: collecting autologous blood for centrifugation, preparing exosome: collecting autologous fat to prepare fat mesenchymal stem cells, and culturing the mesenchymal stem cells, preparing a freeze-drying liquid: mixing the platelet factor-rich plasma and the exosome and adding trehalose for mixing, and performing freeze-drying: performing freeze-drying on the freeze-drying solution. The preparation comprises the exosome freeze-drying powder, hyaluronic acid and saline. The preparation can effectively solve the problems that the existing cosmetic preparation has a poor filling effect, short maintenance time and high immunological rejection response.

Description

technical field [0001] The invention relates to the technical field of freeze-dried preparations, in particular to a freeze-dried exosome powder, a preparation method thereof, and a preparation containing the freeze-dried exosome powder. Background technique [0002] With the growth of age, the face will gradually appear sunken, wrinkled, skin aging, etc., which is a big blow to people who are born with beauty. How to restore their face and skin to a youthful state has also become their pursuit. Aiming at this goal, various facial filling materials and technologies were born, such as hyaluronic acid, umbilical cord blood products, etc., but most of the current filling materials have short maintenance time, poor filling effect or large immune rejection, which leads to patients The problem of poor sensitivity. [0003] Exosomes are nanoscale lipid bilayer vesicles released by exocytosis to the outside of cells, and can exist in various types of extracellular body fluids, such...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/36A61L27/20A61L27/50
CPCA61L27/3834A61L27/3616A61L27/20A61L27/50A61L2400/06C08L5/08
Inventor 高雪华海泉
Owner CHENGDU QINGKE BIOTECH
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