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Low-density lipoprotein adsorbent and preparation method thereof

A low-density lipoprotein and adsorbent technology, applied in chemical instruments and methods, suction devices, other chemical processes, etc., can solve the problems of large volume of low-density lipoprotein adsorption columns and ineffective application of results, and achieve excellent cells. The effect of permeability, excellent clinical application value, and excellent adsorption performance

Active Publication Date: 2018-12-28
WUHAN RUIFA MEDICAL DEVICES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The volume of other adsorption columns on the market is generally 80ml to 350ml. In contrast, the volume of low-density lipoprotein adsorption columns is obviously "extremely large", and it also has a certain risk of use, especially for some patients with less blood volume. patient
Although many domestic scholars have devoted themselves to the study of lipid adsorbents in recent years, the results have not been well applied in practice.

Method used

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  • Low-density lipoprotein adsorbent and preparation method thereof
  • Low-density lipoprotein adsorbent and preparation method thereof
  • Low-density lipoprotein adsorbent and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Select the average pore diameter to be about 13nm, 24nm, 29nm, 41nm, 53nm, 72nm, 104nm, 156nm, 454nm respectively, the agarose gel microspheres with an average particle diameter of about 250 μm, and the dextran sulfate with a molecular weight of 5000, as follows The step is to prepare the adsorbent, and the obtained adsorbent numbers are respectively A1, A2, A3, A4, A5, A6, A7, A8, and A9.

[0027] The preparation method of adsorbent is as follows:

[0028] 1) Drain 10mL of agarose microspheres, transfer them to a conical flask, add 10mL of 1M NaOH solution and 3mL of epichlorohydrin;

[0029] 2) React for 6 hours at 40°C and 150rpm;

[0030] 3) Rinse with a large amount of water, drain and mix with 10mL dextran sulfate buffer solution to adjust the pH to 10-12;

[0031] 4) React at 40°C and 150rpm for 16h;

[0032] 5) Rinse successively with acetic acid buffer solution of pH 4.0, Tris buffer solution of pH 8.0, 1M NaCl solution, and finally with a large amount of wa...

Embodiment 2

[0034] Select dextran sulfate with an average molecular weight of about 1000, 3000, 5000, 8000, 10000, 20000, 40000, 50000, 5000000, respectively, agarose gel microspheres with an average pore diameter of about 70 nm and an average particle diameter of about 250 μm, press The steps described in Example 1 were used to prepare the adsorbents, and the numbers of the obtained adsorbents were respectively B1, B2, B3, B4, B5, B6, B7, B8, and B9.

Embodiment 3

[0036] Choose agarose gel microspheres with an average particle diameter of about 31 μm, 90 μm, 152 μm, 182 μm, 255 μm, 364 μm, 414 μm, and 567 μm, an average pore diameter of 70 μm, and dextran sulfate with a molecular weight of 40,000. Prepare the adsorbent according to the above steps, and the obtained adsorbent numbers are respectively C1, C2, C3, C4, C5, C6, C7, and C8.

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Abstract

The invention discloses a low-density lipoprotein adsorbent and an adsorption column. Agarose gel microspheres of a specific particle size and a specific porosity are adopted as a carrier, are modified by using an epoxy method and are coupled with dextran sulfate ligand molecules of a specific molecular weight. The adsorbent prepared by using the method has a high specific adsorption level and good hemocyte permeability on one hand, on the other hand, has a low non-specific adsorption level, and has excellent clinical application values.

Description

technical field [0001] The invention relates to the field of blood purification of medical instruments, in particular to a low-density lipoprotein adsorbent and a preparation method thereof. Background technique [0002] Blood purification is to lead the patient's blood out of the body and pass it through a purification device to remove some pathogenic substances in it, purify the blood, and achieve the purpose of treating diseases. This process is called blood purification. Blood purification includes: hemodialysis, hemofiltration, hemodiafiltration, plasma exchange, hemoperfusion and immunoadsorption. Blood purification therapy is developed on the basis of hemodialysis. The prototype of hemodialysis has a history of about 100 years, while other blood purification therapies have only appeared for about 30 years. [0003] Hemodialysis, hemofiltration, hemodiafiltration, and plasma exchange are all based on hollow fiber membranes to achieve their therapeutic effects. Hemope...

Claims

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Application Information

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IPC IPC(8): B01J20/24B01J20/28B01J20/30A61M1/36
CPCA61M1/3679B01J20/24B01J20/28021
Inventor 张磊王业富赵艳沈乔李文宇皮云
Owner WUHAN RUIFA MEDICAL DEVICES CO LTD
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