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Determination kit for lipoprotein (a)

A lipoprotein and kit technology, applied in the field of medical biological detection, can solve the problems of low accuracy and precision, unfavorable clinical promotion and use, short storage time, etc., and achieve the effect of accurate results, accurate and reliable detection results, and simple operation

Active Publication Date: 2019-03-29
浙江夸克生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are few kits for detecting serum lipoprotein (a) known on the market, and there are major defects, such as insufficient sensitivity, low accuracy and precision, poor linearity, short storage time, cumbersome operation, Expensive, etc., are not conducive to clinical promotion and use

Method used

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  • Determination kit for lipoprotein (a)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1: Preparation of goat anti-human lipoprotein (a) antibody latex particles

[0039] (1) Preparation of goat anti-human lipoprotein (a) antibody solution: take goat anti-human lipoprotein (a) antibody and dissolve it in Tris-HCl buffer solution with a pH of 7.2 to prepare a goat anti-human antibody with a concentration of 1.0 mL / mL Lipoprotein (a) antibody solution;

[0040] (2) Preparation of latex microsphere suspension: According to the ratio of mass ratio of 1:2, polymethyl methacrylate latex particles with particle diameters of 60-100nm and 100-200nm were dissolved in Tris-HCl buffer solution, the concentration was 1% (w / v), and then sequentially added polyaspartic acid with a concentration of 18mg / mL and N,N-methylene bisacrylamide at a concentration of 10mg / mL, and incubated at room temperature for 45 minutes at high speed. Centrifuge (12000rpm, 30min), discard the supernatant, wash the latex microspheres twice with the same volume of Tris-HCl buffer t...

Embodiment 2

[0042] Embodiment 2: Preparation of goat anti-human lipoprotein (a) antibody latex particles

[0043] Compared with Example 1, only the ratio of goat anti-human lipoprotein (a) antibody solution and latex microsphere suspension in step (3) is different, and the volume ratio in this example is 1:2.

Embodiment 3

[0044] Embodiment 3: Preparation of goat anti-human lipoprotein (a) antibody latex particles

[0045] Compared with Example 1, only the ratio of goat anti-human lipoprotein (a) antibody solution and latex microsphere suspension in step (3) is different, and the volume ratio in this example is 1:3.

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Abstract

The invention provides a determination kit for lipoprotein (a), and relates to the technical field of medical biological detection. The kit comprises reagents R1 and R2, a calibration product and a quality control product, wherein the reagent R1 comprises trihytdroxy methyl-aminomethane, sodium chloride, polyethylene glycol-6000, biological preservative and surfactant, and the reagent R2 comprisestrihytdroxy methyl-aminomethane, goat anti-human lipoprotein (a) antibody latex particles, dibutyl methyl methylbenzene, glycine, dextran sulfate, biological preservative and surfactant. The kit adopts a latex turbidimetric inhibition immunoassay, latex reagent with a mixed particle size is used, a fully automatic biochemical analyser can be used to simultaneously detect a great quantity of samples, so that linearity is good, detection sensitivity, accuracy and accuracy can be improved, detection time is shortened, operation is simple, economic cost is low, and storage time is long.

Description

technical field [0001] The invention relates to the technical field of medical biological detection, in particular to a lipoprotein (a) assay kit. Background technique [0002] Lipoprotein (a) (lipoprotein (a), Lp (a)) is a special independent plasma lipoprotein, the core part is neutral lipid and apoB-100 molecules, and its periphery is surrounded by hydrophilic apoa, The two are covalently linked by disulfide bonds; apoa is a characteristic glycoprotein component of lipoprotein (a), which is mainly composed of a characteristic structure called Kringle. Kringle is composed of 80-114 amino acid residues, depending on The three internal disulfide bonds are stable, which was discovered by Norwegian geneticist Berg in 1963 when he was studying the genetic variation of low-density lipoprotein. It is mainly secreted into the blood after synthesis in the liver, and plays an extremely important role in the process of atherosclerosis. Lipoprotein(a) is closely related to coronary ...

Claims

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Application Information

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IPC IPC(8): G01N33/92G01N33/543G01N33/544G01N33/531
Inventor 陈青松余法建
Owner 浙江夸克生物科技有限公司
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