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Method for biologically removing protein from sea cucumber polysaccharide

A sea cucumber polysaccharide removal technology, which is applied in the direction of drug combination, digestive system, metabolic diseases, etc., can solve the problems of low processing efficiency, serious loss of polysaccharides, and restrictions on the deep processing and application of sea cucumber polysaccharides

Active Publication Date: 2011-05-04
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The savage method uses organic reagents such as chloroform and amyl alcohol to denature and precipitate proteins. The treatment conditions are mild and can better avoid the degradation of polysaccharides, but the treatment efficiency is low and the loss of polysaccharides is serious.
There are deficiencies in the existing methods, which restrict the deep processing and application of sea cucumber polysaccharides to a certain extent

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Prepare a culture medium containing chondroitin sulfate, which contains 0.2% chondroitin sulfate, 0.01% dipotassium hydrogen phosphate, 0.3% sodium nitrate, 0.05% magnesium sulfate, 0.01% calcium chloride and 0.001% iron phosphate , and the rest is seawater with a pH value of 7.5; the culture medium is sterilized and inoculated with the bacteria Alteromonas macleodii, and cultured with shaking at 25°C for 48 hours. The obtained cell culture solution was filtered through diatomaceous earth to remove cells, and the supernatant of the cell culture solution was collected. The obtained supernatant is subjected to ultrafiltration treatment with an ultrafiltration membrane with a molecular weight cut-off of 50 kDa, and the retentate is freeze-dried to obtain a deproteinized polysaccharide product. After the above-mentioned treatment, the protein content of chondroitin sulfate from Haitian ginseng decreased from 2.09±0.10% to 0.33±0.06%, the monosaccharide composition, sulfate ...

Embodiment 2

[0012] Prepare a culture medium containing Haitian melon fucoidan sulfate, its formula is 0.2% Haitian melon fucoidan sulfate, 0.01% dipotassium hydrogen phosphate, 0.3% sodium nitrate, 0.05% magnesium sulfate, 0.01% chlorine Calcium chloride and 0.001% iron phosphate, and the rest is seawater with a pH value of 7.5; the culture medium is sterilized and inoculated with bacteria Alteromonas macleodii, and cultured at 25°C for 48 hours. The obtained cell culture solution was filtered through diatomaceous earth to remove cells, and the supernatant of the cell culture solution was collected. The obtained supernatant is subjected to ultrafiltration treatment with an ultrafiltration membrane with a molecular weight cut-off of 50 kDa, and the retentate is freeze-dried to obtain a deproteinized polysaccharide product. After the above treatment, the protein content of Haitian melon ginseng fucoidan sulfate decreased from 3.58±0.28% to 0.77±0.03%, the monosaccharide composition, sulfate...

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PUM

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Abstract

The invention relates to a method for biologically removing protein from sea cucumber polysaccharide. The method is characterized by comprising the following steps of: inoculating a microorganism into a sea cucumber polysaccharide-containing liquid culture medium; after culturing, removing bacteria from obtained bacterium culture liquid; collecting supernate of the bacterium culture liquid; performing ultrafiltration on the supernate; and drying obtained trapped fluid so as to obtain a protein-removed product of the sea cucumber polysaccharide. The sea cucumber polysaccharide is the mixture of a sea cucumber polysaccharide crude extract, sea cucumber chondroitin sulfate, sea cucumber fucosan dextran sulfate, sea cucumber chondroitin sulfate and sea cucumber fucosan dextran sulfate. Compared with other conventional protein removing methods, the method has the advantages of effectively removing the protein without influencing the original structure of polysaccharide and keeping the polysaccharide well.

Description

technical field [0001] The invention relates to a method for removing protein from polysaccharides, in particular to a method for biologically removing protein from sea cucumber polysaccharides. Background technique [0002] The sea cucumber polysaccharide is an important component of the sea cucumber body wall, which accounts for 5%-10% (mass percentage, the same below) of the dry weight of the sea cucumber body wall. Sea cucumber polysaccharides include sea cucumber chondroitin sulfate and sea cucumber fucoidan sulfate. Among them, sea cucumber chondroitin sulfate has been proved to have anticoagulant, anti-tumor, immunity-enhancing, blood-lipid-lowering and anti-viral and other physiological activities. Sugar sulfate also has various functions such as protecting neural stem cells and preventing gastric ulcers. Sea cucumber polysaccharides have shown good application prospects in functional food and drug development. [0003] Sea cucumber polysaccharides are linked to pr...

Claims

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Application Information

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IPC IPC(8): C08B37/00C08B37/08C08L5/00C08L5/08A61P7/02A61P35/00A61P37/04A61P3/06A61P31/12A61P25/00A61P1/04
Inventor 常耀光薛长湖薛勇宿玮徐杰李兆杰王玉明唐庆娟
Owner OCEAN UNIV OF CHINA
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