43results about How to "Yield advantage" patented technology

The invention relates to a preparation method, a kit and application of hematogenous female autologous germline stem cells, in particular to a technology and a method for producing hematogenous femaleautologous germline stem cells through non-transgenosis by using female autologous blood cells as raw material cells. The preparation method of the hematogenous female autologous germline stem cellscomprises the following steps of: culturing female autologous blood cells in a cell culture solution O1 for 3 days; continuously culturing in a cell culture solution O2 for 6-9 days; continuously culturing in a cell culture solution O3 for 6-9 days; and collecting the hematogenous female autologous germline stem cells. The invention further provides a kit for preparing the hematogenous female autologous germline stem cells. The method and the kit disclosed by the invention have remarkable advantages in the aspects of sampling, production speed, yield and purity of the hematogenous female autologous germline stem cells.

The invention discloses a fermentation production method of sialic acid and belongs to the technical field of bioengineering. The method comprises the following steps: adding a seed liquid into a fermentation tank containing a sterilizing fermentation culture medium; continuously fermenting to produce fermentation liquid containing polysialic acid in a way of replenishing feeding liquid and a hydrogen dioxide solution; then carrying out centrifugal separation, ethanol precipitation and filtration to obtain refined polysialic acid; then, carrying out acidolysis and crystallization on the polysialic acid; and finally, washing and freeze-drying to obtain sialic acid powder. The output of the polysialic acid produced by the method provided by the invention can reach 12.96g / L, the already reported maximum content of the polysialic acid is improved by 87.28%, the hydrolysis rate of polysialic acid is 95%, the content of purified sialic acid reaches 10.01g / L and the purity reaches 94.80%. The method is suitable for industrial promotion and application.

The invention proposes a graphene microchip conductive masterbatch and a preparation method thereof, which is characterized in that graphite powder is mixed with metal tin, the tin is melted under high temperature conditions, the graphite is peeled into graphene microchips by stirring, crushing and shearing, and then mixed with glass The microbeads are quenched and coated, so that graphene is stably and uniformly dispersed in metal tin and coated on glass microspheres. Further, graphene particles stabilized by tin are formed by grinding, dispersed in polymer materials and prepared by a screw extruder. The graphene microchip conductive masterbatch has good dispersibility, and is suitable for the conduction of various conductive plastics, electronic plastics, and photovoltaic plastics.

The invention discloses a seed production technology of cabbage, which adopts the following seed production method: cutting the lateral buds from the nodes, culturing them on the culture medium, and continuously subculturing in the laboratory to obtain the original mother plant; breeding the sterile line: Preparation of sunbeds; preparation of seedling trays; artificial planting of mother plants; maintainer seedlings; planting; pollination; ventilated harvest: remove the row of maintainer plants in the middle of pod formation, increase the row spacing to facilitate ventilation and light transmission, and cut when 70% of the pods turn yellow Make it post-ripening, threshing after drying; male parent breeding: strict bud selection standards, standardized bud peeling and pollination operations, and well-marked pollination; hybrid breeding. The invention provides seed production technology, the yield reaches 65-75kg / mu, the bud rate and bud vigor are as high as more than 85%, and the seed yield is high, laying the foundation for realizing the stable production of cabbage; after planting, whether it is disease resistance, cold resistance Compared with other varieties, it has obvious advantages in terms of physiological shape and output, and is convenient for popularization and planting.

The invention discloses a preparation GS115 / Ac-AMP2 for inhibiting pear-fruit postharvest penicilliosis. The preparation is recombinant yeast GS115 / Ac-AMP2 obtained after Ac-AMP2 antimicrobial peptideis introduced. A construction method for the recombinant yeast GS115 / Ac-AMP2 includes the following steps that optimized Ac-AMP2 genes are constructed onto pPICZalphaA vectors through Xho I and Not Idouble enzyme digestion and T4 ligase, the formed pPICZalphaA / Ac-AMP2 recombinant expression vectors are subjected to linear enzyme digestion, and then are electrically transformed into GS115 competent cells, a single colony PCR is selected in a Zeocin-contained resistant plate, sequencing verification is carried out, and a recombinant pichia yeast GS115 / Ac-AMP2 strain is obtained. The preparation GS115 / Ac-AMP2 can be used for refreshment for inhibiting fruit postharvest diseases.

The invention discloses a preparation GS115 / Ace-AMP1 for inhibiting pear-fruit postharvest penicilliosis. The preparation is recombinant pichia yeast GS115 / Ace-AMP1 obtained after Ace-AMP1 antimicrobial peptide is introduced. A construction method for the recombinant pichia yeast GS115 / Ace-AMP1 includes the following steps that optimized Ace-AMP1 genes are constructed onto pPICZalphaA vectors through Xho I and Not I double enzyme digestion and T4 ligase, the pPICZalphaA / Ace-AMP1 recombinant expression vectors are subjected to linear enzyme digestion, and then are electrically transformed intoGS115 competent cells, a single colony PCR is selected in a Zeocin-contained resistant plate, sequencing verification is carried out, and a recombinant pichia yeast GS115 / Ace-AMP2 strain is obtained.The preparation GS115 / Ace-AMP1 can be used for refreshment for inhibiting fruit postharvest diseases.

The invention provides a method of fast preparing graphene through microwave assisted stirring. Graphite powder is adopted as a raw material, graphite powder and a foamer are mixed, graphite is infiltrated by the foamer and then put into a high mixing machine, high speed stirring is utilized to make graphite in a complete suspension state and a high speed motion state, meanwhile, microwave stripping is adopted to aid so that the foamer among graphite layers can fast react to generate support force, and continuous, fast, environment-friendly and clean preparation of a graphene material can be achieved. According to the method of fast preparing graphene through microwave assisted stirring, the technical effect of continuous, clean and environment-friendly production of graphene is achieved, and industrialized production of graphene dry powder is promoted; further, cutting and stripping are completed through one step in the high mixing machine, graphene is scattered evenly, not only is the layer structure of graphene maintained to the greatest extent, but also the graphene material can be prepared continuously and stably, surface integrity and topological symmetry of graphene are not destroyed, and the quality of graphene finished products is ensured further.

The invention discloses a method for preparing a high-purity and high-dispersion nano magnesium oxide material. The method comprises the following steps: taking magnesium sulfate as a raw material, dispersing the magnesium sulfate with a dispersion emulsifier, magnesium oxide quantum points and ammonia water in a high-pressure homogenizer to form an emulsion, performing high-pressure ejection, and at the same time performing laser sintering at a dispersion state. By adopting the method, the technical defects that a nano material is poor in property and popularization and application of nano magnesium oxide are obstructed as a conventional nano magnesium oxide material is easy to agglomerate and relatively poor in purity are solved, and the technical effect that magnesium oxide quantum points as crystal cores grow rapidly in an isotropic manner to form spherical nano magnesium oxide with high dispersity and high purity is achieved. Furthermore, the scheme disclosed by the invention is simple in preparation process, free of pollution and easy in on-scale industrial production.