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Graesiella sp. WBG-1 as well as isolation and screening method and application thereof

A technology of oil balls and algae, applied in the fields of microalgae resources, energy and environmental protection, which can solve the problems of high energy consumption, easy pollution, and poor environmental adaptability, and achieve high separation efficiency, wide application range, and pertinence strong effect

Active Publication Date: 2016-05-04
WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Nevertheless, there is no successful case of commercial production of microalgal biomass fuel so far (Steenberghen and López, 2008, Journal of Cleaner Production 16 (5): 577-590; Stephens, Rossetal., 2010, Trends in Plant Science; Wijffels and Barbosa, 2010, Science 329 (5993) :796-799), the bottleneck problem that causes this present situation has three aspects: 1, the high cost of microalgae biodiesel has seriously hindered the commercial production of microalgae biofuel (Torzillo, Pushparajetal., 2003, Biotechnology and Bioprocess Engineering 8 (6 ):338-348), 2. The genetic regulation and physiological regulation basis of microalgae oil synthesis and accumulation are not deep, and the engineering technology foundation of microalgae large-scale cultivation is weak, etc. (WijffelsandBarbosa, 2010, Science329(5993): 796- 799; Li Jian, Zhang Xuecheng, etc., 2012, Science Bulletin 1(1):23-31); 3. Lack of high-quality algal species that are easy to cultivate, grow fast, have high environmental tolerance, and are suitable for large-scale cultivation (Beal, Smith et al. ,2011, Bioenergyresearch4(1):36-60; Liu, Chen et al., 2011, Progress in Natural Science: Materials International21(4):269-276; Li Tao, Li Aifen et al., 2011, China Biotechnology Journal 31(004):98-105; Li, Pribyletal., 2013, Biotechnology and Bioengineering 110(1):97-107; Guccione, Biondi et al., 2014, Biotechnology for Biofuels 7(1):84)
Searching for high-quality algae species from abundant natural microalgae resources is expected to solve the problems of low yield, easy pollution, high harvesting energy consumption, and poor environmental adaptability encountered in the current large-scale cultivation of microalgae. An efficient approach to material production costs (Stephens, Ross et al., 2010, Trends in Plant Science; Wijffels and Barbosa, 2010, Science329(5993): 796-799)

Method used

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  • Graesiella sp. WBG-1 as well as isolation and screening method and application thereof
  • Graesiella sp. WBG-1 as well as isolation and screening method and application thereof
  • Graesiella sp. WBG-1 as well as isolation and screening method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1: the separation and screening method of Oleococcus Graesiellasp.WBG-1, its steps are:

[0043] A method for separation and screening of Oleococcus Graesiellasp.WBG-1, the steps of which are:

[0044] 1. Water sample collection: A plankton collector made of 400-mesh gauze tulle draws the word "∞" below the water surface to enrich algae cells. The collected samples are placed in sterile bottles, and a small amount of concentrated BG-11 is added for cultivation. Base, and then stand for 3 days under low light, and then carry out the separation of algal species. The water samples in this example were collected from Yongsheng County, Yunnan Province.

[0045] 2. Separation and purification:

[0046] (1) Search and separation of target algae cells: Find the algae cells to be separated (target algae cells) under a 10X or 20X microscope, then bring the micropipette (0.1mm inner diameter) close to the target cell and lift it up quickly. The siphon action rapidly ...

Embodiment 2

[0060] Example 2: Expanded cultivation of Oleococcus Graesiellasp.WBG-1 and its use in the production of microalgae oil (biodiesel feedstock).

[0061] A kind of application of oleococcus Graesiellasp.WBG-1 in microalgae biodiesel raw material, its steps are:

[0062] 1. Reactor preparation: first wipe the inner surface of the incubator with 75% ethanol three times, then inject 40 liters of culture water that has been boiled and sterilized and cooled to room temperature, and put the boiled and sterilized vent tube and sand core gas diffuser into the incubator In the process, turn on the gas source and start ventilating (air flow rate 3L / min), and the gas to be ventilated is air / carbon dioxide mixed gas (CO 2 / air=1 / 99, V / V) and sterilized by filtration through a 0.22 μm pore size filter.

[0063] 2. Medium preparation: According to the improved BG11 medium formula 1, add the medium components one by one, stir thoroughly after each component is added, and then add the next com...

Embodiment 3

[0069]Example 3: Expanded culture of Oleococcus Graesiellasp.WBG-1 and its use in the production of microalgae protein.

[0070] A kind of application of Oleococcus Graesiellasp.WBG-1 in microalgae protein production, its steps are:

[0071] 1. Reactor preparation: first wipe the inner surface of the incubator with 75% ethanol three times, then inject 40 liters of culture water that has been boiled and sterilized and cooled to room temperature, and put the boiled and sterilized vent tube and sand core gas diffuser into the incubator In the process, turn on the gas source and start to ventilate (air flow rate 3L / min), and the gas to be ventilated is air / carbon dioxide mixture (CO 2 / air=1 / 99, V / V) and sterilized by filtration through a 0.22 μm pore size filter.

[0072] 2. Medium preparation: According to the improved BG11 medium formula 2, add the medium components one by one, stir thoroughly after each component is added, and then add the next component to prevent precipitat...

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Abstract

The invention discloses Graesiella sp. WBG-1 as well as an isolation and screening method and an application thereof. The algal strain grows fast, is high in adaptability to pH change, and has the characteristics of large cells, proneness to sedimentation, resistance to protozoa swallow and the like, which are suitable for cultivation in large scale. The Graesiella sp. WBG-1 accumulates a large amount of grease in cells under the low-nitrogen cultivation condition, so that the Graesiella sp. WBG-1 can be used as raw materials of microalgae biodiesel; a large amount of protein is accumulated in the cells of the Graesiella sp. WBG-1 under the high-nitrogen cultivation condition, the Graesiella sp. WBG-1 can be applied to production of microalgae protein accordingly; besides, the Graesiella sp. WBG-1 can fix exogenous carbon dioxide efficiently under the higher pH condition, thereby being capable of being applied to biological fixation of flue gas carbon dioxide.

Description

technical field [0001] The present invention relates to the fields of microalgae resources, energy and environmental protection, more specifically to a strain of Oleococcus Graesiellasp. The use of coccus Graesiellasp.WBG-1 in microalgae biodiesel feedstock, microalgae protein production, and microalgae fixation of exogenous carbon dioxide. Background technique [0002] Microalgae are widely distributed in nature and can produce a variety of metabolites. Separation and screening of fine algae species, development of new uses of microalgae and new production processes are the main tasks of microalgae biotechnology research. In the 1960s, Japan realized the large-scale cultivation and production of Chlorella (Tsukada and Kawahara, 1977, Biological Solar Energy Conversion 363-365). So far, Spirulina (Shimamatsu, 2003, Hydrobiologia512: 39-44), Dunaliella (Oren, 2005, AquaticBiosystems 1 (1): 2-15), Haematococcus (Boussiba and Vonshak, 1991, Plant & Cell Physiology 32 (7) :10...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12P7/64C12R1/89
CPCC12N1/12C12P7/6463C12N1/125C12R2001/89Y02E50/10
Inventor 李夜光温小斌耿亚洪张丹王中杰丁奕
Owner WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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