Precise fermentation culture medium for edible fungus liquid strains and application

A technology for fermentation medium and liquid strains, applied in application, cultivation, plant cultivation and other directions, can solve the problems of changes in medium composition, pH and other properties, affecting the stability of strains, complex raw materials in the process, etc. , The effect of inducing effective proliferation and uniform growth rate

Pending Publication Date: 2018-11-23
INST OF AGRI RESOURCES & ENVIRONMENT SHANDONG ACADEMY OF AGRI SCI
View PDF5 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Natural medium is good for the growth of microorganisms, the disadvantage is that the composition is complex and inconsistent, and fungi are prone to spores on natural medium
At present, the medium used for the production of edible fungus liquid strains is mostly made of natural raw materials such as potatoes, bran, corn flour, soybean meal or soybean flour, such as the edible mushrooms disclosed in Chinese patent documents CN 106831037 A, CN 106497798 A, CN 107805096 A, etc. Bacteria liquid culture medium is prepared from raw materials such as potatoes, corn flour, wheat bran, soybean flour, soybean meal, yeast powder, peptone, etc. Although the medium made of such raw materials can ensure the growth of bacteria, the disadvantage is that the process Complex raw materials often require pretreatment, and second, often due to different batch sources of natural raw materials, the composition, pH and other properties of the medium will change, which will affect the stability and uniformity of the strain and further affect the consistency of later cultivation management. Coherence

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Precise fermentation culture medium for edible fungus liquid strains and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A method for producing precise liquid strains of Flammulina velutipes:

[0034] (1) Preparation of Flammulina velutipes parent species: Get the preserved bacterial strain and inoculate it on the enriched PDA plate medium, and cultivate it in the dark at 22°C for 7 days; the formula of the enriched PDA plate medium is: potato 200g / L (boiled juice) , wheat bran 20g / L (boiled juice), plum (boiled juice) 30g / L, glucose 20g / L, yeast extract powder 3g / L, potassium dihydrogen phosphate 1g / L, magnesium sulfate 0.5g / L, agar 20g / L.

[0035] (2) Preparation of Flammulina velutipes shaker bottle seed liquid: take the flat plate covered with mycelium in step (1), draw concentric circles with a diameter of 6cm and 7cm on the back of the flat plate, and use a diameter of 0.5cm to punch two concentric circles Bacteria discs were taken between circles, and inoculated into 500mL culture bottles containing 200mL edible fungus liquid strain precision fermentation medium, 5 slices were inoc...

Embodiment 2

[0040] A method for producing precise liquid strains of Pleurotus eryngii:

[0041] (1) Preparation of the parent species of Pleurotus eryngii: Inoculate the preserved bacterial strain on the enriched PDA plate medium, and culture at 22° C. in the dark for 7 days. The formula of enriched PDA plate medium is: potato 200g / L (boiled juice), wheat bran 20g / L (boiled juice), plum (boiled juice) 20g / L, glucose 20g / L, yeast extract powder 3g / L, potassium dihydrogen phosphate 1g / L, magnesium sulfate 0.5g / L, agar 20g / L.

[0042] (2) Preparation of Pleurotus eryngii shake bottle seed liquid: get the flat plate covered with mycelia in step (1), draw concentric circles with a diameter of 6 cm and 7 cm on the back of the flat plate, and use a hole punch with a diameter of 0.5 cm on both sides Bacteria discs were taken between the two concentric circles, and inoculated into a 500mL culture bottle containing 200mL edible fungus liquid strain precision medium, 10 pieces were inoculated in ea...

Embodiment 3

[0047] A method for producing precise liquid strains of Shijiji mushroom:

[0048] (1) Preparation of the parent species of Pleurotus jiris: the preserved strain was inoculated on the enriched PDA plate medium, and cultured at 22° C. in the dark for 7 days. The formula of enriched PDA plate medium is: potato 200g / L (boiled juice), wheat bran 20g / L (boiled juice), plum (boiled juice) 20g / L, glucose 20g / L, yeast extract powder 3g / L, potassium dihydrogen phosphate 1g / L, magnesium sulfate 0.5g / L, agar 20g / L.

[0049] (2) The preparation of the seed liquid of the shake bottle of jiji mushroom: take the flat plate covered with mycelia in the step (1), draw concentric circles with a diameter of 6 cm and 7 cm on the back of the flat plate, and use a hole punch with a diameter of 0.5 cm on both sides. Bacteria discs were taken between the two concentric circles, and inoculated into a 500mL culture bottle containing 200mL edible fungus liquid strain precision medium, 10 pieces were ino...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a precise fermentation culture medium for edible fungus liquid strains and application. The medium comprises a carbon source with monotonous components, a nitrogen source withmonotonous components, glycerin, KH2PO4, K2HPO4, MgSO4.7H2O, FeSO4, gibberellin, sodium diacetate, VB1, VB2 and GPE. The inventor of the medium discovers for the first time that the precise culture medium can be obtained by blending all kinds of compounds clear in component, and is suitable for growth of edible fungus mycelia, which overcomes the technical prejudice, that edible fungi need to becultured through a culture medium prepared from natural components, of technicians in the field. Since the components of the culture medium are controllable, mycelium growth speed is uniform after theliquid strains of different batches obtained through culturing are inoculated in solid compost, and production management is easily standard and programmable; the new conception is provided for reducing fluctuations in industrialized production of the edible fungi.

Description

technical field [0001] The invention relates to a precision culture medium for edible fungus liquid strains and its application, in particular to a liquid culture medium for industrialized cultivation of edible fungi and its preparation method and application, belonging to the technical field of edible fungi cultivation. Background technique [0002] The industrialized cultivation of edible fungi is the industrial production mode with the most modern agricultural characteristics, and it is the only way for the future development of the edible fungus industry. Special production strains are the basis of factory production and are directly related to the output, quality and benefit of the final product. At present, the industrialized seed production industry in the world has embarked on the road of specialization and industrialized mass production. Liquid strains have become the development trend of the edible fungus seed production industry because of their short culture tim...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/20A01G18/40
CPCA01G18/20A01G18/40
Inventor 黄春燕宫志远杨鹏李瑾韩建东谢红艳姚强
Owner INST OF AGRI RESOURCES & ENVIRONMENT SHANDONG ACADEMY OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap