Method for extracting total alkaloids in semen strychni

An extraction method and a technology of total alkaloids, which are applied in the extraction of effective components of traditional Chinese medicine, and in the field of extracting total alkaloids of Nuxychnium from the traditional Chinese medicine Nuxychnium, can solve the problems of low safety of strychnine

Active Publication Date: 2013-04-10
ZHANGZHOU PIEN TZE HUANG PHARM +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported in the literature that strychnine and strychnine are used to gavage mice respectively, and the LD50 of the two is 3.27mg/kg and 233mg/kg respectively, so it can be known that the safety of strychnine is very low, if It c...

Method used

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  • Method for extracting total alkaloids in semen strychni
  • Method for extracting total alkaloids in semen strychni
  • Method for extracting total alkaloids in semen strychni

Examples

Experimental program
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Effect test

Embodiment 1

[0155] Take 1000g of Nuxychnium, crush it into a coarse powder, add 10 times the amount of 1% sulfuric acid, heat and reflux twice, 1.5 hours each time, combine the extracts, filter, add sodium hydroxide to the filtrate to adjust the pH to 6, filter, and the filtrate Adsorb on a D-101 macroporous adsorption resin column with a flow rate of 2Bv / hour, first elute with 2 times the column volume of deionized water, discard the eluent, then elute with 1 times the column volume of 3% hydrochloric acid, discard the eluent Remove the liquid, and finally elute with 4 times the column volume of 50% ethanol, collect the eluate, and concentrate the eluate under reduced pressure at 70°C and -0.09Mpa to a concentrated solution with a relative density of 1.18-1.20 at 50°C , add sodium hydroxide to adjust the pH value to 10, add 1 / 10 of the amount of diatomaceous earth, stir well, evaporate to dryness, add 6 times chloroform to the obtained solid and reflux twice at 85°C for 1 hour each time, ...

Embodiment 2

[0158] Take 1000g of Nuxychnium, crush it into coarse powder, add 5 times the amount of 0.5% sulfuric acid, heat and reflux 3 times, 0.5 hours each time, combine the extracts, filter, add sodium hydroxide to the filtrate to adjust the pH value to 7, filter, and the filtrate Adsorb on a D-101 macroporous adsorption resin column with a flow rate of 3Bv / hour, first elute with 1 times the column volume of deionized water, discard the eluent, then elute with 0.5 times the column volume of 5% hydrochloric acid, discard the eluent Remove the liquid, and finally elute with 2 times the column volume of 95% ethanol, collect the eluate, and concentrate the eluate under reduced pressure at 70°C and -0.09Mpa to a concentrated solution with a relative density of 1.18-1.20 at 50°C , add sodium hydroxide to adjust the pH value to 11, add 1 / 20 of the amount of diatomaceous earth, mix well, evaporate to dryness, add 10 times chloroform to the obtained solid and reflux once at 85°C for 2 hours ea...

Embodiment 3

[0161]Take 1000g of Nuxyche, crush it into a coarse powder, add 12 times the amount of 5% hydrochloric acid, heat and reflux once, for 2 hours each time, combine the extracts, filter, add sodium hydroxide to the filtrate to adjust the pH value to 8, filter, and the filtrate Adsorb on a D-101 macroporous adsorption resin column with a flow rate of 1Bv / hour, first elute with 3 times the column volume of deionized water, discard the eluent, then elute with 0.5 times the column volume of 5% sulfuric acid, discard the eluent Remove the liquid, and finally use 6 times the column volume 80% ETOH·0.5% H 2 SO 4 Elution, collect the eluate, concentrate the eluate under reduced pressure at 70°C and -0.09Mpa to a concentrated solution with a relative density of 1.18-1.20 at 50°C, add sodium hydroxide to adjust the pH value to 8, add Mix 1 / 5 of the amount of diatomaceous earth, evaporate to dryness, add 3 times chloroform to the obtained solid, stir and extract 3 times at 85°C, each time ...

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Abstract

The present invention discloses a method for extracting total alkaloids in semen strychni, and belongs to the technical field of drugs. The method comprises the steps of extracting semen strychni crude powder with acid, filling on a column, separating, and purifying to remove some of strychnine. The prepared semen strychni total alkaloid content is above 50%, wherein the content ratio of alkaloids and strychnine is about 2:1, thereby effectively reducing the toxicity and improving the safety of clinical medication. The extraction method can efficiently separated high purity semen strychni total alkaloids from the traditional Chinese medicine of semen strychni.

Description

technical field [0001] The invention relates to a method for extracting active ingredients of traditional Chinese medicine, in particular to a method for extracting total alkaloids of Nuxychnium chinensis from the traditional Chinese medicine Nuxychnium chinensis, belonging to the technical field of medicines. Background technique [0002] Nuxychia, also known as Pancha, was first recorded in "Benzao Compendium". It is bitter in taste, cold in nature, and highly poisonous. It returns to the liver and spleen meridian. It is clinically used for diseases such as rheumatic obstinate numbness, numbness and paralysis, bruises, carbuncle, sequelae of poliomyelitis, and rheumatoid arthritis; but its highly toxic characteristics seriously affect its clinical application. The agent is good at resolving serious illnesses". The therapeutic dose of Nuxychnami is 0.3-0.6g, and overdose is prone to poisoning. How to use Nuxychnium reasonably and safely has become one of the research hotsp...

Claims

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Application Information

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IPC IPC(8): A61K36/56A61P29/00
Inventor 张保献胡杰
Owner ZHANGZHOU PIEN TZE HUANG PHARM
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