The invention discloses a method for detecting the content of denatonium benzoate in toy materials, which comprises preparing standard stock solutions, intermediate working standard solutions and serial concentration standard solutions respectively, preprocessing samples to be tested, and performing high performance liquid chromatography detection , using acetonitrile-water (30:70, v / v) solution as the extraction solvent, after ultrasonic extraction, constant volume, after filtration, gradient elution is carried out with acetonitrile-water as the mobile phase, and a C-18 column is used as the chromatographic column, With a photodiode array detector, the detection wavelength of denatonium benzoate is 227.4nm, the detection limit is 2.66ng (based on 3 times the signal-to-noise ratio), the average recovery rate is 99.3%, and the method precision is 0.81%-2.36%. This method has low detection limit and high precision, and is suitable for the determination of the bittering agent denatonium benzoate content in this field. It can improve the level of management of liquid or viscous toys in my country, protect the legitimate rights and interests of children and consumers in China, and at the same time promote the development of toys in my country. The smooth export of products is of great significance.