Method for determining naringin and denatonium benzoate serving as bitterants in finger painting inkpad for children by ultra-high performance liquid chromatography

An ultra-high performance liquid phase and naringin technology, applied in the detection field, can solve problems such as the complex matrix of children's finger painting ink pads, children's physical and mental health hazards, and sensitivity that cannot meet the requirements.

Active Publication Date: 2019-11-12
上海海关机电产品检测技术中心
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Problems solved by technology

[0005] The matrix of children’s finger painting ink pads is complex. In order to avoid interference, it is necessary to add an extraction solvent with a high dilution factor for extraction. The standard recommended concentration of denatonium benzoate is 4 mg/kg, which is a challenge for the sensitivity of the instrument and method. The sensitivity of the instrument and assay method of above-mentioned denatonium benzoate can not meet the requirement
[0006] Children's finger painting inkpad is one of the finger painting pigments. Children use their fingers to dip

Method used

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  • Method for determining naringin and denatonium benzoate serving as bitterants in finger painting inkpad for children by ultra-high performance liquid chromatography
  • Method for determining naringin and denatonium benzoate serving as bitterants in finger painting inkpad for children by ultra-high performance liquid chromatography
  • Method for determining naringin and denatonium benzoate serving as bitterants in finger painting inkpad for children by ultra-high performance liquid chromatography

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Embodiment

[0037] An ultra-high performance liquid chromatography method for determining the bitter agents naringin and denatonium benzoate in children's fingerprint inkpad, including the following steps:

[0038] (1) Preparation of test solution: accurately weigh the sample to be tested in a conical flask with stopper, add methanol -0.02 mol / LKH 2 PO 4 Extraction solvent, the volume of the extraction solvent is 50 times the mass of the sample, ultrasonic extraction, take the clear liquid and filter it with a filter membrane to obtain the filtrate, which is used as the test solution for ultra-high performance liquid chromatography analysis;

[0039] (2) Standard solution preparation: mix standard solutions of naringin and denatonium benzoate with different concentration gradients;

[0040] (3) Ultra-high performance liquid chromatography is used for determination, and the chromatographic conditions are:

[0041] Chromatographic column: C18;

[0042] Column temperature: 30-40℃;

[0043] Mobile phase...

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Abstract

The invention discloses a method for determining naringin and denatonium benzoate serving as bitterants in finger painting inkpad for children by ultra-high performance liquid chromatography. According to the method, two bitterants in a sample of the finger painting inkpad of children are extracted in an ultrasonic extracting mode by taking methyl alcohol-0.02 mol/L KH2PO4 (30:70, v/v) as a solvent; and the two bitterants are quantified through an external standard method and are analyzed through the UPLC. The linear ranges of the naringin and the denatonium benzoate are both 0.1 to 5 mg/L (R2is greater than or equal to 0.99), and the detection limits (S/N>3) are respectively 0.75 and 1.15 mg/kg. The mark recovery rate ranges of the naringin and the denatonium benzoate in the finger painting inkpad for the children at three concentration levels are 86.7 to 108.4 percent; and the relative standard deviation (RSD, n=6) is less than 5.0 percent.

Description

Technical field [0001] The invention relates to a detection method, in particular to a method for determining the bitter agents naringin and denatonium benzoate in children's finger painting ink pad by ultra-high performance liquid chromatography. Background technique [0002] Aversive agent (Aversive agent), also known as aversive agent, anti-feeding agent, is added to products to make the taste or smell bitter to prevent people, especially children, from inhaling or ingesting toxic and harmful substances, rodents and birds Chemical agents widely used to destroy facilities. Denatonium benzoate, also known as Bitrex, whose structure is composed of benzoic acid groups and denatonium groups, is the most bitter compound currently known and a typical representative of bittering agents. Bitter essence is chemically stable, inexpensive, easy to obtain, and easily soluble in water. In addition, its Median lethal dose (LD50) of mammals is 841 mg / kg body weight, and only a very small amo...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/36G01N30/86
CPCG01N30/02G01N30/06G01N30/36G01N30/8675G01N2030/062G01N2030/027
Inventor 望秀丽卫碧文高欢倪彬彬
Owner 上海海关机电产品检测技术中心
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