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Application of denatonium benzoate in preparing bactericide for preventing and controlling plant diseases caused by phytopathogen

A technology of denatonium benzoate and plant pathogenic bacteria, which is applied in the field of bactericidal active compounds to achieve the effects of high residue, large pollution and fast resistance

Active Publication Date: 2016-07-13
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] At present, there is no report on the inhibitory effect of denatonium benzoate on agricultural pathogenic bacteria at home and abroad

Method used

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  • Application of denatonium benzoate in preparing bactericide for preventing and controlling plant diseases caused by phytopathogen
  • Application of denatonium benzoate in preparing bactericide for preventing and controlling plant diseases caused by phytopathogen
  • Application of denatonium benzoate in preparing bactericide for preventing and controlling plant diseases caused by phytopathogen

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Experimental program
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Effect test

Embodiment 1

[0042] 1. Experimental materials

[0043] Denatonium benzoate was purchased as finished product from Sigma Corporation. Weigh 8.92g of denatonium benzoate in 200ml of ultrapure water to prepare a 0.1M mother solution, filter and sterilize and store at 4°C until use.

[0044] The phytopathogenic bacteria used in this experiment were strains stored at 4°C in the laboratory, and the medium used was potato medium (PDA for short).

[0045] PDA medium formula: potato (peeled) 200g, glucose 20g, agar 15g, tap water 1000ml, natural PH

[0046] Preparation method: wash and peel the potatoes, weigh 200g, cut into small pieces, add water and boil until rotten (boil for 20-30 minutes, it can be pierced by a glass rod), filter it into a beaker with eight layers of gauze, add 15-20g of agar, add 20g of glucose, stir evenly, fully dissolve and then cool down to make up water to 1000ml, after subpackaging, sterilize at 121°C for 20 minutes, cool down and set aside.

[0047] 2. Experimental...

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Abstract

The invention discloses application of denatonium benzoate in preparing a bactericide for preventing and controlling plant diseases caused by phytopathogen.It is proved through indoor toxicity measurement that denatonium benzoate has good inhibitory activity to phytopathogen.Serving as a bactericide, denatonium benzoate has the advantages of being efficient and low in toxicity and meeting the requirement for chemically preventing and controlling plant diseases.At present, bactericide resistance of pathogen is enhanced as a great number of bactericides are used, and the traditional bactericides pollute the environment seriously, are high in residual amount and directly threat food safety of human.Denatonium benzoate is a small molecule compound which is degradable, free of pollution and environmentally friendly, is low in bactericide resistance, safe to non-target organisms, people and livestock and capable of ensuring high quality of agricultural products, fruits and vegetables, meets the requirement for sustainable development and has wide research and market application prospects.

Description

technical field [0001] The invention relates to compounds with fungicidal activity, in particular to the use of denatonium benzoate in the preparation of fungicides for preventing and treating plant diseases caused by plant pathogenic bacteria. Background technique [0002] In the new century, mankind is facing more challenges and crises, among which problems such as population growth, resource shortage, environmental pollution, ecological damage and frequent natural disasters are becoming more and more prominent, which seriously hinder the sustainable development of agriculture, and agricultural production is therefore burdened. Unprecedented pressure. According to the estimates of the Food and Agriculture Organization of the United Nations, the annual loss of grain and cotton production in the world is more than 10% due to diseases. Plant diseases can not only reduce the yield of crops, but also seriously threaten the quality and safety of agricultural products and their ...

Claims

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Application Information

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IPC IPC(8): A01N37/46A01P3/00
CPCA01N37/46
Inventor 梁文星张佳男刘琳周善跃
Owner QINGDAO AGRI UNIV
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