Use of curcumin in the preparation of fungicides for preventing and treating plant diseases caused by plant pathogenic bacteria
A technology for plant pathogenic bacteria and plant diseases, applied in the field of fungicidal active compounds, can solve problems such as reports of curcumin inhibitory effects, and achieve the effects of broad research and market application prospects, large environmental pollution, and high residues
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[0044] 1. Experimental materials
[0045] Curcumin is a finished product purchased from Sigma. Weigh 7.37g of curcumin in 20ml of dimethyl sulfoxide to prepare a 1M mother solution, filter and sterilize and store at 4°C for later use.
[0046] The phytopathogenic bacteria used in this experiment were strains stored at 4°C in the laboratory, and the medium used was potato medium (PDA for short).
[0047]PDA medium formula: potato (peeled) 200g, glucose 20g, agar 15g, tap water 1000ml, natural PH
[0048] Preparation method: wash and peel the potatoes, weigh 200g, cut into small pieces, add water and boil until rotten (boil for 20-30 minutes, it can be pierced by a glass rod), filter it into a beaker with eight layers of gauze, add 15-20g of agar, add 20g of glucose, stir evenly, fully dissolve and then cool down to make up water to 1000ml, after subpackaging, sterilize at 121°C for 20 minutes, cool down and set aside.
[0049] 2. Experimental method
[0050] using the growt...
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