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Use of 5-azacytidine in the preparation of a fungicide for preventing and treating plant diseases caused by plant pathogens

A technology for plant pathogenic bacteria and plant diseases, which is applied in the field of fungicides for plant diseases to achieve the effects of high residue, large pollution and fast resistance.

Active Publication Date: 2020-05-26
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] At present, there is no report on the inhibitory effect of 5-azacytidine on agricultural pathogenic bacteria at home and abroad

Method used

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  • Use of 5-azacytidine in the preparation of a fungicide for preventing and treating plant diseases caused by plant pathogens
  • Use of 5-azacytidine in the preparation of a fungicide for preventing and treating plant diseases caused by plant pathogens
  • Use of 5-azacytidine in the preparation of a fungicide for preventing and treating plant diseases caused by plant pathogens

Examples

Experimental program
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Effect test

Embodiment 1

[0041] 1. Experimental materials

[0042] 5-azacytidine was purchased from Sigma as a finished product. Weigh 2g of 5-azacytidine in 50ml of dimethyl sulfoxide to prepare a 0.2M mother solution, filter and sterilize and store at 4°C until use.

[0043] The phytopathogenic bacteria used in this experiment were strains stored at 4°C in the laboratory, and the medium used was potato medium (PDA for short).

[0044] PDA medium formula: potato (peeled) 200g, glucose 20g, agar 15g, tap water 1000ml, natural pH.

[0045] Preparation method: wash and peel the potatoes, weigh 200g, cut into small pieces, add water and boil until rotten (boil for 20-30 minutes, it can be pierced by a glass rod), filter it into a beaker with eight layers of gauze, add 15-20g of agar, add 20g of glucose, stir evenly, fully dissolve and then cool down to make up water to 1000ml, after subpackaging, sterilize at 121°C for 20 minutes, cool down and set aside.

[0046] 2. Experimental method

[0047] usin...

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Abstract

The invention discloses application of 5-azacytidine to preparation of a fungicide for preventing and controlling plant diseases caused by plant pathogenic bacteria. An indoor toxicity test proves that the 5-azacytidine has good inhibition activity on plant pathogenic fungi. The 5-azacytidine is used as the fungicide and has the advantages of high efficiency and low toxicity and is suitable for requirements of chemical prevention and control on the plant diseases. At present, a lot of fungicides are utilized so that the drug resistance of the pathogenic bacteria is enhanced; and a traditionalfungicide has great pollution to the environment and high residues and directly threatens the food safety of human. However, the 5-azacytidine is a degradable, pollution-free and environment-friendlysmall molecule compound, and has poor drug resistance and is safe to non-target organisms and human and livestock; high quality of agricultural products, fruits and vegetables can be ensured, and sustainable development requirements can be met; the 5-azacytidine has a wide research and market application prospect.

Description

technical field [0001] The invention belongs to the technical field of fungicidal active compounds, and specifically relates to the use of 5-azacytidine in the preparation of fungicides for preventing and treating plant diseases caused by plant pathogenic bacteria. Background technique [0002] Since the 21st century, with the advancement of science and technology and the improvement of social productivity, human beings have created unprecedented wealth. At the same time, problems such as population growth, resource shortage, environmental pollution, ecological damage and frequent natural disasters have become increasingly prominent, seriously hindering agricultural production. As a result, agricultural production is under unprecedented pressure. According to the estimates of the Food and Agriculture Organization of the United Nations, the annual loss of grain and cotton production in the world is more than 10% due to diseases. Plant diseases can not only reduce the yield o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/66A01P3/00
CPCA01N43/66
Inventor 梁文星李雯雯王雪胡继承
Owner QINGDAO AGRI UNIV
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