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Method for extracting purified total alkaloid of strychnos nux-vomica and application thereof in pharmacy

A technology of total alkaloids and extraction method, which is applied in the field of extraction and purification of total alkaloids of purifying St. Chin seeds, can solve problems such as high cost, difficulty in realizing large-scale industrial production, and large loss of alkaloid components, and achieves toxicity reduction, resistance Tumor pharmacological action, effect of easy industrial production

Inactive Publication Date: 2010-08-18
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It must be pointed out that although it is possible to completely remove strychnine, the cost is high, and the loss of other alkaloid components is also large, and it is difficult to realize large-scale industrial production. Therefore, removing part of strychnine is more feasible and has application prospects
There is no report about the method of removing part of strychnine from the total alkaloids of Strychnos chinensis

Method used

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  • Method for extracting purified total alkaloid of strychnos nux-vomica and application thereof in pharmacy
  • Method for extracting purified total alkaloid of strychnos nux-vomica and application thereof in pharmacy
  • Method for extracting purified total alkaloid of strychnos nux-vomica and application thereof in pharmacy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: the method for extracting and purifying the total alkaloids of Nuxebra chinensis:

[0030] (1) take Nuxychia, pulverize, sieve, make coarse powder, take by weighing 200g coarse powder;

[0031] (2) Heat and reflux extraction with 70% ethanol, 1000 mL each time, extract for 1 hour, extract 3 times in total, filter, combine the filtrates three times, recover ethanol under reduced pressure, and obtain alcoholic extract;

[0032] (3) Sonicate with 400 mL of 1 mol / L hydrochloric acid, centrifuge at 4000 rpm for 10 min, and take out the supernatant;

[0033] (4) Regulate pH to 12 with sodium hydroxide, use 2000mL dichloromethane to extract and merge in 5 times, remove dichloromethane by vacuum drying, accurately weigh the weight to obtain the total alkaloid powder of St. The content of strychnine accounts for 52% of the total alkaloids of Nuxychnium;

[0034] (5) ultrasonically dissolving the total alkaloid powder of Nuxebra chinensis obtained above in 2000mL ...

Embodiment 2

[0037] Embodiment 2: the method for extracting and purifying the total alkaloids of Nuxebra chinensis:

[0038] (1) take Nuxychia, pulverize, sieve, make coarse powder, take by weighing 200g coarse powder;

[0039] (2) Heat and reflux extraction with 90% ethanol, 2000 mL each time, extract for 2 hours, extract twice in total, filter, combine the filtrates, recover ethanol under reduced pressure, and obtain alcoholic extract;

[0040] (3) ultrasonically dissolve with 0.2mol / L hydrochloric acid 1000mL, and suction filter to obtain the filtrate;

[0041] (4) Use sodium hydroxide to adjust the pH to 14, use 1000mL of dichloromethane to extract and merge three times, recover under reduced pressure to remove the dichloromethane, accurately weigh the weight, and obtain the total alkaloids of Nuxica chinensis, which are determined by HPLC. Dinin content accounts for 46% of the total alkaloids of Strychnis;

[0042] (5) ultrasonically dissolving the total alkaloid powder of Nuxebra c...

Embodiment 3

[0045] Embodiment 3: the method for extracting and purifying the total alkaloids of Nuxebra chinensis:

[0046] (1) take Nuxynum, pulverize, sieve, make coarse powder, take by weighing 100g coarse powder;

[0047] (2) Heat and reflux extraction with 50% ethanol, 1000 mL each time, extract for 1 hour, extract 4 times in total, filter, combine filtrates, recover ethanol under reduced pressure, and obtain alcoholic extract;

[0048] (3) ultrasonically dissolve with 0.5mol / L hydrochloric acid 400mL, and suction filter to obtain the filtrate;

[0049] (4) Regulate the pH to 10 with sodium hydroxide, extract and combine in 4 times with 500mL dichloromethane, reclaim under reduced pressure to remove the dichloromethane, accurately weigh the weight, and obtain the total alkaloids of Nuxica chinensis, which are determined by HPLC The content of dinine accounts for 44% of the total alkaloids of Nuxebra chinensis;

[0050] (5) ultrasonically dissolving the total alkaloid powder of Nuxe...

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Abstract

The invention discloses a method for obtaining purified total alkaloid of strychnos nux-vomica by removing partial strychnine in the total alkaloid of the strychnos nux-vomica, which comprises the following steps: extracting with a certain concentration of ethanol, dissolving the extract with hydrochloric acid ; adjusting the pH value to be alkaline after dissolving; carrying out extracting and pressure reduction on dichloromethane to remove solvent to obtain the total alkaloid of the strychnos nux-vomica; ultrasound dissolving total alkaloid powder of strychnos nux-vomica with ethanol; carrying out pressure reduction and concentration to a certain volume; centrifuging the concentrated solution, taking supernate, refrigerating and standing to remove precipitate; and drying the supernate to obtain the purified total alkaloid of strychnos nux-vomica. The content of strychnine in the total alkaloid of strychnos nux-vomica extracted by the method is remarkably reduced; and in addition, the method has safe extraction process, low cost and is beneficial to industrial scale production. Proved by the evaluation result of a pharmacological test, the effects of analgesia, anti-inflammation, immunity and antitumor activity of the purified total alkaloid of strychnos nux-vomica are remarkably superior to the effects of total alkaloid of strychnos nux-vomica and the toxicity is remarkably reduced.

Description

1. Technical field [0001] The invention relates to a method for extracting and purifying the active ingredients of traditional Chinese medicine, in particular to a method for extracting and purifying the total alkaloids of the purified nuxychonna after removing part of strychnine and its application in pharmacy. 2. Technical Background [0002] Strychnounux-vomica L., also known as Strychnounux-vomica L., was first recorded in "Compendium of Materia Medica Volume Eighteen". Efficacy, traditional Chinese medicine is often used to treat sore throat, swelling poison, rheumatic arthralgia, fractures, facial paralysis, myasthenia gravis and other diseases. "Compendium of Materia Medica" states that it can cure "typhoid fever, sore throat, and eliminate lumps" as a medicine for promoting blood circulation and removing blood stasis. Pills" and other Chinese patent medicines. [0003] The total alkaloids are the most important effective parts in Nuxychnium. The current research sh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/56A61P25/20A61P29/00A61P35/00A61P37/02A61K131/00
Inventor 蔡宝昌陈军陈志鹏
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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