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Limit test method for strychnine in Huatuo Zaizao pills

An inspection method, the technology of strychnine, applied in the field of qualitative detection of drugs, can solve the problems of ineffective control of Huatuo Zaizao pill product quality and lack of quality control methods for Nuxebra nuxe, to ensure effectiveness, safety, and operation Convenience and consistent quality

Inactive Publication Date: 2016-07-06
GUANGZHOU BAIYUNSHAN QIXING PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The quality standard of Huatuo Zaizao Pills is recorded on page 848 of the 2015 edition of the Pharmacopoeia of the People's Republic of China. There are only TLC identifications of Rhizoma Chuanxiong, Evodia Evodia, and Borneolum, and there is a lack of quality control methods for Nuxebra chinensis, which cannot be effectively controlled. The product quality of Hua Tuo Zai Zao Pills

Method used

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  • Limit test method for strychnine in Huatuo Zaizao pills

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for checking the limit of strychnine in Huatuo Zaizao Pills uses thin-layer chromatography to measure the limit of strychnine, comprising the steps of:

[0026] Preparation of the test solution: take Huatuozaizao pills, grind them, weigh 16g of powder, then add 50ml of chloroform, 5ml of ethanol, 6ml of ammonia water, ultrasonically treat for 30-60min; filter, evaporate the filtrate to dryness, add 6% of the residue HNO 3 Heat 20ml of the solution on a water bath to dissolve, adjust the pH value to 11-12 with ammonia water, shake and extract 3 times with chloroform, 20ml each time, combine the extraction solutions, evaporate to dryness, add 1ml of chloroform to the residue to dissolve, As the test solution.

[0027] Reference substance solution preparation: take strychnine reference substance, add chloroform to make a solution containing 2mg per 1ml, as the reference substance solution.

[0028] Limit inspection: draw 12 μl of the test solution and 10 μl of t...

Embodiment 2

[0032] The characteristics of this embodiment are: the difference is that in the preparation step of the test solution: the residue is added with 6% H 2 SO 4 The solution was heated to dissolve. Other specific operation steps are the same as embodiment 1.

Embodiment 3

[0034] The characteristics of this embodiment are: the difference is that in the preparation step of the test solution: use 5% NaHCO 3 Adjust the pH value; in the limit check step: use toluene-acetone-ethanol-concentrated ammonia test solution (volume ratio 5:5:0.6:0.4) as the developing agent. Other specific operation steps are the same as embodiment 1.

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Abstract

The invention discloses a limited inspection method for strychnine in Huatuo reconstituted pills, comprising: 1) taking Huatuo reconstituted pills, weighing the powder, adding extraction solvent, ethanol, ammonia water, ultrasonic treatment; filtering, evaporating the filtrate to dryness, Add acid solution to the residue, heat to dissolve, adjust pH value, shake and extract with organic solvent, evaporate to dryness, add chloroform to dissolve the residue, and use it as the test solution; 2) take strychnine reference substance, add chloroform Make a solution as the reference substance solution; 3) draw the test solution and the reference substance solution, respectively point on the same silica gel G thin-layer plate, develop with a developing agent, take it out, dry it, spray it with a chromogenic agent, and use it for testing. In the chromatogram of the reference substance, there should be no spots at the position corresponding to the chromatogram of the reference substance, or the spots should be smaller than those of the reference substance. The invention can well control the quality of Huatuo Zaizao Pills, ensure the stable, uniform and controllable quality of the pills, and ensure the effectiveness and safety of human medicine.

Description

technical field [0001] The invention relates to a limited inspection method for strychnine in Huatuo Zaizao Pills, and relates to the technical field of qualitative detection of medicines. Background technique [0002] Hua Tuo Zaizao Pill is a compound preparation of traditional Chinese medicine. It is a drug that has been on the market for many years and has definite clinical efficacy. The prescription of Hua Tuo Zaizao Pills consists of Rhizoma Chuanxiong, Evodia Evodia, Borneol, and Nuxychnium. Its function cures mainly for promoting blood circulation and removing blood stasis, resolving phlegm and dredging collaterals, promoting qi circulation and relieving pain. It is used for the recovery period and sequelae of apoplexy caused by phlegm and blood stasis blocking collaterals. Symptoms include hemiplegia, spasm and numbness, crooked mouth and eyes, and slurred speech. [0003] Traditional Chinese medicine Fuzheng Guben first recommends Shenqi, while Hua Tuo Zaizao Pil...

Claims

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Application Information

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IPC IPC(8): G01N30/90
CPCG01N30/90
Inventor 伏宝香彭中芳锶景希彭丹婷
Owner GUANGZHOU BAIYUNSHAN QIXING PHARMA
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