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Microfluidic chip for cotinine quick detection and preparation method thereof

A microfluidic chip and cotinine technology, which is applied in the photoplate process of the patterned surface, biological testing, measuring devices, etc., can solve the problems of difficult to meet the experimental requirements, expensive, large instrument volume, etc.

Inactive Publication Date: 2011-04-27
FUDAN UNIV
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  • Description
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Problems solved by technology

Gas chromatography and other methods have a long cycle, high cost, and high requirements for staff. Due to the large size and high price of the instrument, it is difficult to achieve real-time online monitoring
In many cases, the sensitivity and stability of thin-layer chromatography and other methods are difficult to meet the experimental requirements.
The above traditional detection methods all involve expensive and sophisticated experimental equipment, professional technicians and experimental sites. Therefore, for the rapid detection and analysis of harmful components in smoke components, new technologies are needed to achieve fast, sensitive, and high-throughput. Testing needs
[0005] So far, there is no practical rapid detection system for harmful components in representative flue gas at home and abroad

Method used

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  • Microfluidic chip for cotinine quick detection and preparation method thereof
  • Microfluidic chip for cotinine quick detection and preparation method thereof
  • Microfluidic chip for cotinine quick detection and preparation method thereof

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Embodiment Construction

[0034] Substrate preparation. Put the silicon chip into Piranha solution (98% concentrated sulfuric acid: 30% hydrogen peroxide = 7: 3) and boil for 15 min. Rinse five times with deionized water, blow dry with nitrogen, and bake at 200 °C for 30 min.

[0035] Spray paint. Pour Microchem's SU-8 glue (the same below) on the center of the silicon wafer, and tilt the edge of the silicon wafer so that SU-8 covers most of the silicon wafer. Let it stand for 15 minutes while eliminating the air bubbles generated during the pouring process. Use a spin coater (Spin-Coater KW-4A, Chemat Technology, Inc.) to carry out two progressive spin coatings: 500 rpm spin coating for 25 s, 3000 rpm spin coating for 50 s, so that the glue distribution is relatively uniform, static Leave it on for 10 minutes to alleviate the edge protrusion effect.

[0036] Soft bake. Gradually raise to 95°C at a rate of 5°C / min on a hot plate, during which time the temperature is maintained at 65°C, 95°C, and 6...

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Abstract

The invention belongs to the technical field of biological analysis detection, in particular to a microfluidic chip for cotinine quick detection and a preparation method thereof. In the chip, optical transparent polydimethylsiloxane and the like can be used as materials by adopting a molding method; and the chip mainly comprises a sample reaction microchannel layer, a valve control layer and a substrate layer. The chip comprises a sample enriching and immune analysis module and a signal acquisition module, wherein the sample enriching and immunity analysis module consists of a plurality of immune chromatographic column micro-analysis chambers with nanoliter volumes, and each analysis chamber fixes cotinine antibody protein or antigen to realize quick detection on the cotinine in samples from different sources. The chip has the characteristics of quickness, high efficiency and low cost, is convenient to carry, is easy to control automatically, can complete automatic signal acquisition, remote transmission and signal analysis, and is suitable for quick detection on the ingredient in the air and medical clinical detection.

Description

technical field [0001] The invention belongs to the technical field of biological analysis and detection, in particular to a microfluidic chip for rapid detection of cotinine, and provides a preparation method of the chip. Background technique [0002] Smoking is harmful to health. Many reports at home and abroad have studied the harm of various components in cigarettes to the human body, and it has developed into a public health problem. The governments of various countries have paid full attention to the personal and social problems caused by exposure to smoking and second-hand smoke, and made huge economic investments. The rapid detection of harmful components in smoke is of great importance to the development of tobacco products, human health and environmental protection. practical significance. [0003] Nicotine (1-methyl-2-(3-pyridyl)pyrrolidine, commonly known as nicotine, English name is nicotine, referred to as Nic, molecular formula is C10H14N2). It is one of the...

Claims

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Application Information

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IPC IPC(8): G01N33/545G01N30/56G01N33/52G03F7/00
Inventor 隋国栋刘思秀赵望张金玲刘超
Owner FUDAN UNIV
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