Biological detection microfluidic chip based on immune reaction and preparation method thereof

Abstract

The invention belongs to the technical field of biological analysis and detection, in particular relates to a biological detection microfluidic chip based on immune reaction and a preparation method thereof. The chip takes optically transparent polydimethylsiloxane as a material and comprises a sample channel layer, a valve control layer and a substrate layer. The chip contains a sample enrichment and immunoassay module which comprises one or a plurality of nanolitre-size immune chromatographic column micro-analysis rooms, wherein each analysis room is fixed with antibody protein or antigen and is used for realizing rapid field detection of a plurality of antigen samples, such as bacteria, viruses and various toxins. The invention has the characteristics of fast speed, high efficiency, convenient carrying, low cost and easy automation control, and is capable of finishing automatic signal acquisition, remote transmission and signal analysis and suitable for rapid field detection and large-range remote detection of a plurality of antigens with the existence of commercial antibodies.

Description

technical field [0001] The invention belongs to the technical field of biological analysis and detection, in particular to a microfluidic chip used for on-site rapid biological detection and analysis, and provides a preparation method of the chip. Background technique [0002] In the late 1970s and early 1980s, labeling immunoassay methods based on antigen-antibody specific reactions and high sensitivity were developed rapidly. The principle of radioimmunoassay was applied, and non-radioactive tracers such as enzymes, fluorescent dyes, and luminescent agents were used instead Antigens or antibodies are labeled with radioactive elements, and some new labeling immunoassay techniques have emerged. According to the difference between the labeled probe and the corresponding detection method, it can be divided into enzyme-linked immunosorbent assay, fluorescent immunoassay, chemiluminescence immunoassay, electrochemical immunoassay, colloidal gold-labeled immunoassay, etc. These ...

AI Technical Summary

Problems solved by technology

[0004] However, ELISA routine immunoassay usually takes several hours or even more than a day, the operation process is complicated, and a large amount of expensive immunological reagents are consumed

Moreover, the detection equipment is relatively large, and it is often accompanied by many shortcomings such as non-specific adsorption, which makes it difficult to meet the requirements of on-site inspection.

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