Biological detection microfluidic chip based on immune reaction and preparation method thereof

A microfluidic chip and immune reaction technology, applied in biological testing, chemical instruments and methods, material inspection products, etc., can solve the problems of consumption of immune reagents, non-specific adsorption, large detection equipment, etc.

Inactive Publication Date: 2010-11-17
FUDAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, ELISA routine immunoassay usually takes several hours or even more than a day, the operation process is complicated, and a large amount of expensive immunological reagents are consumed
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Method used

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  • Biological detection microfluidic chip based on immune reaction and preparation method thereof
  • Biological detection microfluidic chip based on immune reaction and preparation method thereof
  • Biological detection microfluidic chip based on immune reaction and preparation method thereof

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Embodiment Construction

[0031] 1. Substrate preparation. Put the silicon chip into Piranha solution (98% concentrated sulfuric acid: 30% hydrogen peroxide = 7: 3) and boil for 15 min. Rinse five times with deionized water, blow dry with nitrogen, and bake at 200 °C for 30 min.

[0032] 2. Shake the paint. Pour Microchem's SU-8 glue (the same below) on the center of the silicon wafer, hold the edge of the silicon wafer with your hand to tilt it and rotate it slowly, so that SU-8 covers most of the silicon wafer. Let it stand for 15 minutes to make SU-8 preliminarily flattened and eliminate the air bubbles generated during the pouring process. Use a spin coater (Spin-Coater KW-4A, Chemat Technology, Inc.) to carry out two progressive spin coatings: 500 rpm spin coating for 15 s, 3000 rpm spin coating for 30 s, so that the glue distribution is relatively uniform, static Leave it on for 10 minutes to alleviate the edge protrusion effect.

[0033]3. Soft baking. The purpose of soft baking is to volat...

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Abstract

The invention belongs to the technical field of biological analysis and detection, in particular relates to a biological detection microfluidic chip based on immune reaction and a preparation method thereof. The chip takes optically transparent polydimethylsiloxane as a material and comprises a sample channel layer, a valve control layer and a substrate layer. The chip contains a sample enrichment and immunoassay module which comprises one or a plurality of nanolitre-size immune chromatographic column micro-analysis rooms, wherein each analysis room is fixed with antibody protein or antigen and is used for realizing rapid field detection of a plurality of antigen samples, such as bacteria, viruses and various toxins. The invention has the characteristics of fast speed, high efficiency, convenient carrying, low cost and easy automation control, and is capable of finishing automatic signal acquisition, remote transmission and signal analysis and suitable for rapid field detection and large-range remote detection of a plurality of antigens with the existence of commercial antibodies.

Description

technical field [0001] The invention belongs to the technical field of biological analysis and detection, in particular to a microfluidic chip used for on-site rapid biological detection and analysis, and provides a preparation method of the chip. Background technique [0002] In the late 1970s and early 1980s, labeling immunoassay methods based on antigen-antibody specific reactions and high sensitivity were developed rapidly. The principle of radioimmunoassay was applied, and non-radioactive tracers such as enzymes, fluorescent dyes, and luminescent agents were used instead Antigens or antibodies are labeled with radioactive elements, and some new labeling immunoassay techniques have emerged. According to the difference between the labeled probe and the corresponding detection method, it can be divided into enzyme-linked immunosorbent assay, fluorescent immunoassay, chemiluminescence immunoassay, electrochemical immunoassay, colloidal gold-labeled immunoassay, etc. These ...

Claims

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Application Information

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IPC IPC(8): B01L3/00G01N33/53
Inventor 隋国栋刘思秀赵望刘超张金玲
Owner FUDAN UNIV
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