Integrated multifunctional controllable cell control and analysis micro-fluidic chip and application thereof
A microfluidic chip, a multi-functional technology, applied in the field of biomedical applications, can solve the problems of difficult integration of high-throughput analysis, micro-analysis and controllable cell manipulation functions, and achieve a simple structure, simplified operation process, and easy production. Effect
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Embodiment 1
[0020] Example 1: Applying the microfluidic chip of the present invention to cell stress analysis.
[0021] (1) The design of the integrated multifunctional controllable cell manipulation and analysis microfluidic chip of the present invention:
[0022] The designed microfluidic chip can conduct orderly manipulation, controllable packaging and formation of cells in the chip, complete the design and functional verification of the above three modules, and then integrate the flow paths and structural characteristics of the three modules into one chip and connect with the channel. The chip includes a cell ordered manipulation module (asymmetric curved channel), a cell controllable wrapping module (the oil phase channel on both sides and the middle water phase channel required for droplet formation), a cell array formation module and a simple laminar flow based diffusion The principle of concentration gradients forms channels.
[0023] (2) Preparation of microfluidic chip:
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Embodiment 2
[0031] Example 2: The microfluidic chip of the present invention is used for performance analysis of antitumor drugs.
[0032] (1) The design of the integrated multifunctional controllable cell manipulation and analysis microfluidic chip of the present invention:
[0033] The designed microfluidic chip can conduct orderly manipulation, controllable wrapping and formation of cells in the chip, complete the design and functional verification of the above three modules, and then integrate the flow paths and structural characteristics of the three modules into one on-chip and channel-connected. The chip includes a cell ordered manipulation module (asymmetric curved channel), a cell controllable wrapping module (the oil phase channel on both sides and the middle water phase channel required for droplet formation), a cell array formation module and a simple laminar flow based diffusion The principle of concentration gradients forms channels.
[0034] (2) Preparation of microfluidi...
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