Micro-fluidic chip for chemiluminescence detection and detection method thereof

Abstract

A micro-fluidic chip for chemiluminescence detection and a detection method thereof relate to a miniaturized total analysis system. The micro-fluidic chip is provided with a chip which is provided with at least a micro-channel. Each micro-channel is successively composed of a pre-storage liquid micro-channel, a sample micro-channel and a reaction micro-channel. Two ends of the micro-channel are respectively provided with an inlet and an outlet. The detection method comprises the following steps: the micro-channels of the micro-fluidic chip are completely connected and it is ensured that interfaces are enclosed; driving force is applied on an outlet and / or inlet of each micro-channel, and solutions at different parts of the micro-channels successively flow through the reaction micro-channels under the action of driving force; when all the solutions are flowing through the reaction micro-channels, chemiluminescence occurs; and light emitted undergoes signal acquisition by the use of a photomultiplier or CCD camera so as to realize chemiluminescence detection of an object to be measured in a sample. The micro-fluidic chip has a simple structure, is easy to manufacture, is simple to operate and fast to detect, has little external interference but is free of cross contamination. Through parallel multiple micro-channels, parallel detection of multiple samples is realized.

Description

technical field [0001] The invention relates to a micro total analysis system, in particular to a microfluidic chip for chemiluminescence detection and a detection method thereof. Background technique [0002] At present, there are two main trends in the development of in vitro diagnostics: one is high sensitivity and integrated integration, that is, high-sensitivity large-scale fully automatic equipment in large hospitals or institutions to achieve high-precision diagnosis and analysis of diseases; the other One is miniaturization and bedside diagnosis, that is, on-site diagnosis and rapid analysis are realized through small automated equipment (Liu Daozhi. China Medical Device Information. 2014. Status and development trend of China's in vitro diagnostic industry). At present, our country has a large population. Due to the aggravation of the aging phenomenon and the outbreak of various epidemics, people's morbidity has increased sharply. However, relying entirely on large-...

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Problems solved by technology

At present, the instruments and supporting reagents of the chemiluminescence analysis method used in domestic clinical testing mainly rely on imports, which are expensive and consume a lot of reagents, resulting in high testing costs.

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