Microfluidic microbead array chip and application thereof in virus analysis

A microbead array chip and microfluidic technology, applied in the field of biological application of the micro-total analysis system, can solve the problem of increased operational difficulty and complexity, unsuitable analysis of large-scale samples or high-throughput samples, and limitations of microchips Technology development and application issues, to achieve the effect of simplifying the preparation process and making the preparation simple

Active Publication Date: 2010-05-19
香港城市大学深圳研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Microfluidic chip is an emerging technology developed in the 1990s, but its main disadvantage is that it is not suitable for the analysis of large-scale samples or high-throughput samples.
Although some research groups have adopted the method of combining multiple microfluidic channels in parallel for multi-sample analysis, the increase in channels will inevitably be restricted by chip size and microfabrication technology, and each channel needs to be injected separately, thus Increased operational difficulty and complexity of experiments
These shortcomings have limited the further development and application of microchip technology to a certain extent.

Method used

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  • Microfluidic microbead array chip and application thereof in virus analysis
  • Microfluidic microbead array chip and application thereof in virus analysis
  • Microfluidic microbead array chip and application thereof in virus analysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1, performance analysis of highly sensitive virus recognition based on quantum dot-labeled microfluidic bead array chip:

[0015] 1. Design and synthesis of hepatitis B virus genotype-specific probes: Search the Genbank database, compare the sequences of various types of HBV virus genomes, screen out various types of specific probes from the S genes with large sequence differences, and synthesize various types of probes. Needle and modify the chemical groups at the 5-end or 3-end. Table 1 shows the designed HBV B, C, D and E genotype-specific typing probes and corresponding modification groups.

[0016]

[0017] 2. Biofunctional modification of microbeads: 15 micron polystyrene microspheres are used as the solid phase interface for probe immobilization, and the specific steps are as follows: (1) take 100 microliters of avidin-modified microbeads with a concentration of 1% on In a centrifuge tube, wash twice with 100 microliters of affinity eluent (20mM Tris p...

Embodiment 2

[0020] Example 2, the sensitivity of detecting HBV virus in serum by microfluidic microbead array chip based on quantum dot marker recognition:

[0021] 1. Design and synthesis of hepatitis B virus genotype-specific probes: Search the Genbank database, compare the sequences of various types of HBV virus genomes, screen out various types of specific probes from the S genes with large sequence differences, and synthesize various types of probes. Needle and modify the chemical groups at the 5-end or 3-end. Table 1 shows the designed HBV B, C, D and E genotype-specific typing probes and corresponding modification groups.

[0022] 2. Biofunctional modification of microbeads: 15 micron polystyrene microspheres are used as the solid phase interface for probe immobilization, and the specific steps are as follows: (1) take 100 microliters of avidin-modified microbeads with a concentration of 1% on In a centrifuge tube, wash twice with 100 microliters of affinity eluent (20mM Tris pH 7....

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Abstract

The invention discloses a microfluidic microbead array chip and application thereof in virus analysis, and belongs to the field of biological application of a miniaturized total analysis system. The microfluidic microbead array chip consists of a microbead fixed microstructure array, a microbead transport channel, a reagent transport channel and a microstructure array coverage channel. When high-sensitive virus analysis is performed, virus molecules in a serum sample is prepared into a biotin doped target RNA sequence through PCR and in vitro transcription process, and flows to a testing areaof the chip to react with a functional microbead array; function modified microspheres can specifically identify and capture target sequences; a quantum dot labeled reagent are introduced; surfaces of micro-particles are combined with quantum dots because of specifically capturing; and detection and quantitation are performed by a fluorescence imaging method. The microfluidic microbead array chipprovided by the invention has the advantages of simple process, high detecting sensitivity and quick detection, and provides an effective studying and detecting means for high-sensitive virus analysis.

Description

technical field [0001] The invention relates to the biological application field of a micro total analysis system, in particular to the design of a microfluidic microbead array chip and its application in highly sensitive virus analysis. Background technique [0002] Viruses have always been one of the main killers of human health in the long history of human beings. Therefore, the monitoring and diagnosis of pathogens and their harm to the human body are also global health issues that have attracted much attention. Today, with the rapid development of society and economy, new viruses are coming out of nature and "discovering" human beings. Since 1994 alone, more than 30 viruses have appeared, such as Ebola hemorrhagic fever, hepatitis F and G, Andes virus, Faki virus, Billydo virus, Whitewater Wadi virus, etc., which have made the medical community helpless . In our country, in the few years after entering the 21st century, pathogen contamination incidents broke out frequ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12Q1/70C12Q1/68G01N21/64
Inventor 杨梦苏张何徐涛
Owner 香港城市大学深圳研究院
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