Nano graphene oxide protein complex, preparation method and applications thereof

A technology of nano-graphite oxide and protein complexes, applied in biochemical equipment and methods, chemical instruments and methods, microorganisms, etc., can solve problems such as poor stability, achieve low cost, avoid enzymatic digestion and hydrolysis, and simple methods

Active Publication Date: 2012-07-25
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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Problems solved by technology

However, the stability of the above-mentioned graphene oxide in sali...

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  • Nano graphene oxide protein complex, preparation method and applications thereof
  • Nano graphene oxide protein complex, preparation method and applications thereof
  • Nano graphene oxide protein complex, preparation method and applications thereof

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Embodiment Construction

[0025] In view of the above-mentioned deficiencies in the prior art and the urgent needs in the actual application of the protein process, the present invention studies and proposes a nano-graphene oxide protein complex for the deficiencies in the prior art, and the nano-graphene oxide protein complex is for protein adsorption. A composite structure on the surface of nano-graphene oxide, wherein the nano-graphene oxide contains carboxylic acid functional groups and polyethylene glycol groups, and the protein contains fluorescein molecules.

[0026] The fluorescein molecule is a fluorescein isothiocyanate molecule or a fluorescein molecule with an isothiocyanate group. In addition, the nanometer graphene oxide protein complex has a projection size of 5-300nm and a thickness of 1-4nm.

[0027] The method for preparing the nano-graphene oxide protein complex proposed by the present invention is to compound the protein labeled with fluorescein and the graphene oxide modified by po...

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Abstract

The invention discloses a nano graphene oxide protein complex, a preparation method of the nano graphene oxide protein complex and applications of the nano graphene oxide protein complex. The complex is of a complex structure that protein is absorbed on the surface of the nano graphene oxide. The nano graphene oxide protein complex is prepared as follows: conducting fluorescence labeling on protein drugs, modifying the nano graphene oxide protein by polyethylene glycol (PEG), and combining and gathering the protein drugs on the nano graphene oxide through a physical adsorption mode. The nano graphene oxide protein complex designed by the invention has extremely high loading efficiency and stability; in an in-vitro physiological environment, the protein physically absorbed on the surface of the graphene oxide can be released gradually, the enzyme digestion and the hydrolysis to the protein, caused by the protease can be avoided effectively, and simultaneously, in the cells, the protein can be effectively delivered into the cells from the surface of the graphene oxide. According to the invention, the effects of regulating and controlling the physiological activities of the cells can be improved, the powerful guaranteeing can be provided for the medical application under the physiological conditions; and according to the preparation method provided by the invention, the method is simple and convenient, the cost is low and the volume production is facilitated.

Description

technical field [0001] The invention relates to a nano-graphene oxide protein compound and its preparation method and application, in particular to the application of nano-graphene oxide as a protein carrier in delivering protein into cells to regulate cell biological activity. Background technique [0002] Because proteins can regulate apoptosis and intracellular signal transduction, and affect the process of cell metabolism, they can be used as a new type of molecular pharmacological target, such as the treatment of tumors and other diseases through protein drugs. Different from chemical drugs, although protein drugs have good biological activity and low toxicity, they are easily degraded by proteases existing in the organism, and cannot be effectively delivered to cells or diseased tissues, resulting in low bioavailability and greatly reducing the quality of protein. The efficacy of drugs limits their application in the field of biomedicine. [0003] Graphene oxide, as a...

Claims

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Application Information

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IPC IPC(8): C07K17/14C12N5/00C12N5/09
Inventor 张智军沈贺
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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