Nano graphene oxide protein complex, preparation method and applications thereof

Abstract

The invention discloses a nano graphene oxide protein complex, a preparation method of the nano graphene oxide protein complex and applications of the nano graphene oxide protein complex. The complex is of a complex structure that protein is absorbed on the surface of the nano graphene oxide. The nano graphene oxide protein complex is prepared as follows: conducting fluorescence labeling on protein drugs, modifying the nano graphene oxide protein by polyethylene glycol (PEG), and combining and gathering the protein drugs on the nano graphene oxide through a physical adsorption mode. The nano graphene oxide protein complex designed by the invention has extremely high loading efficiency and stability; in an in-vitro physiological environment, the protein physically absorbed on the surface of the graphene oxide can be released gradually, the enzyme digestion and the hydrolysis to the protein, caused by the protease can be avoided effectively, and simultaneously, in the cells, the protein can be effectively delivered into the cells from the surface of the graphene oxide. According to the invention, the effects of regulating and controlling the physiological activities of the cells can be improved, the powerful guaranteeing can be provided for the medical application under the physiological conditions; and according to the preparation method provided by the invention, the method is simple and convenient, the cost is low and the volume production is facilitated.

Description

technical field [0001] The invention relates to a nano-graphene oxide protein compound and its preparation method and application, in particular to the application of nano-graphene oxide as a protein carrier in delivering protein into cells to regulate cell biological activity. Background technique [0002] Because proteins can regulate apoptosis and intracellular signal transduction, and affect the process of cell metabolism, they can be used as a new type of molecular pharmacological target, such as the treatment of tumors and other diseases through protein drugs. Different from chemical drugs, although protein drugs have good biological activity and low toxicity, they are easily degraded by proteases existing in the organism, and cannot be effectively delivered to cells or diseased tissues, resulting in low bioavailability and greatly reducing the quality of protein. The efficacy of drugs limits their application in the field of biomedicine. [0003] Graphene oxide, as a...

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Problems solved by technology

However, the stability of the above-mentioned graphene oxide in saline solutions such as phosphate buffer or cell culture medium is still poor

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